Tuberculosis and Respiratory Diseases

  • 제76권3호
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  • Pages.120-126
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  • 2014
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  • 1738-3536(pISSN)
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  • 2005-6184(eISSN)
대한결핵및호흡기학회 (The Korean Academy of Tuberculosis and Respiratory Diseases)
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Apigenin and Wogonin Regulate Epidermal Growth Factor Receptor Signaling Pathway Involved in MUC5AC Mucin Gene Expression and Production from Cultured Airway Epithelial Cells

  • Sikder, Md. Asaduzzaman (Department of Pharmacology, Chungnam National University School of Medicine) ;
  • Lee, Hyun Jae (Department of Pharmacology, Chungnam National University School of Medicine) ;
  • Ryu, Jiho (Department of Pharmacology, Chungnam National University School of Medicine) ;
  • Park, Su Hyun (Department of Pharmacology, Chungnam National University School of Medicine) ;
  • Kim, Ju-Ock (Pulmonology Section, Department of Internal Medicine, Chungnam National University Hospital, Chungnam National University School of Medicine) ;
  • Hong, Jang-Hee (Department of Pharmacology, Chungnam National University School of Medicine) ;
  • Seok, Jeong Ho (Department of Pharmacology, Chungnam National University School of Medicine) ;
  • Lee, Choong Jae (Department of Pharmacology, Chungnam National University School of Medicine)
  • 투고 : 2013.11.12
  • 심사 : 2014.01.23
  • 발행 : 2014.03.30
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초록

Background: We investigated whether wogonin and apigenin significantly affect the epidermal growth factor receptor (EGFR) signaling pathway involved in MUC5AC mucin gene expression, and production from cultured airway epithelial cells; this was based on our previous report that apigenin and wogonin suppressed MUC5AC mucin gene expression and production from human airway epithelial cells. Methods: Confluent NCI-H292 cells were pretreated with wogonin or apigenin for 15 minutes or 24 hours and then stimulated with epidermal growth factor (EGF) for 24 hours or the indicated periods. Results: We found that incubation of NCI-H292 cells with wogonin or apigenin inhibited the phosphorylation of EGFR. The downstream signals of EGFR such as phosphorylation of MEK1/2 and ERK1/2 were also inhibited by wogonin or apigenin. Conclusion: The results suggest that wogonin and apigenin inhibits EGFR signaling pathway, which may explain how they inhibit MUC5AC mucin gene expression and production induced by EGF.

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