YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Anti-inflammatory Effect and Inhibition of Melanin Biosynthesis of Clematis mandshurica

위령선 추출물의 항염활성 및 멜라닌 생성 억제효과

  • Kim, Ye Rim (College of Pharmacy, Sookmyung Women's University) ;
  • Hong, Yun Jung (College of Pharmacy, Sookmyung Women's University) ;
  • Yang, Ki Sook (College of Pharmacy, Sookmyung Women's University)
  • 김예림 (숙명여자대학교 약학대학) ;
  • 홍윤정 (숙명여자대학교 약학대학) ;
  • 양기숙 (숙명여자대학교 약학대학)
  • Received : 2013.11.30
  • Accepted : 2014.02.17
  • Published : 2014.02.28
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Abstract

Clematis mandshurica (Ranunculaceae) has traditionally been used as a remedy for antidiuretic, antifungal, rheumatic conditions and alleviate pain. We carried out to evaluate the anti-oxidative effect, anti-inflammatory effect and anti-melanogenic effect of ethanol extract and solvent fractions of Clematis mandshurica. The ethanol extract and the dichloromethane fraction of Clematis mandshurica showed an anti-oxidative effect in DPPH assay, the inhibitory activity of nitric oxide (NO) production in lipopolysaccharide (LPS) activated RAW 264.7 cell, and melanin synthesis and tyrosinase activity of B16F10 melanoma cells. They reduced NO production and melanin content in a dose-dependent manner at concentrations of $2.5{\sim}10{\mu}g/ml$. They also suppressed iNOS and tyrosinase protein and m-RNA expressions dose dependently, assayed by western blot analysis and RT-PCR experiment.

Keywords

References

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