Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Inhibition of SMP30 Gene Expression Influences the Biological Characteristics of Human Hep G2 Cells

  • Zhang, Sheng-Chang (Anatomy Teaching and Research Section, Guangxi Traditional Chinese Medical University) ;
  • Liang, Ming-Kang (Anatomy Teaching and Research Section, Guangxi Traditional Chinese Medical University) ;
  • Huang, Guang-Lin (Anatomy Teaching and Research Section, Guangxi Traditional Chinese Medical University) ;
  • Jiang, Kui (Anatomy Teaching and Research Section, Guangxi Traditional Chinese Medical University) ;
  • Zhou, Su-Fang (Department of Biochemistry and Molecular Biology, Pre-Clinical School Guangxi Medical University) ;
  • Zhao, Shuang (Department of Pathophysiology, Youjiang Medical University for Nationalities)
  • Published : 2014.02.01
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Abstract

Senescence marker protein 30 (SMP30), a hepatocellular carcinoma (HCe) associated antigen had been identified by our research group. To study its mechanisms of regulation and associations with the occurrence and development of HCe, we inhibited expression by RNAi technique, and observed effects on the biological characteristics of Hep G2 cells. In cell viability assays, cell growth in the experimental group (with siRNA transfection) was elevated. In Transwell invasion assays, compared with blank and control groups, numbers of invading cells in the experimental group were significantly increased, whereas in apoptosis assays, the percentage apoptosis demonstrated no differences, but after UV irradiation, that in the experimental group was higher than the other two groups. In a word, SMP30 can inhibit the proliferation and invasion of human hepatoma cells and thus can be regarded as a cancer suppressive factor.

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References

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