Asian Pacific Journal of Cancer Prevention

  • 제15권8호
  • /
  • Pages.3457-3460
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  • 2014
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  • 1513-7368(pISSN)
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  • 2476-762X(eISSN)
아시아태평양암예방학회 (Asian Pacific Journal of Cancer Prevention)
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Molecular Markers for Patients with Thymic Malignancies: not Feasible at Present?

  • Avci, Nilufer (Department of Medical Oncology, Uludag University Faculty of Medicine) ;
  • Cecener, Gulsah (Department of Medical Biology, University of Uludag, Institute of Health Sciences) ;
  • Deligonul, Adem (Department of Medical Oncology, Uludag University Faculty of Medicine) ;
  • Erturk, Elif (Department of Medical Biology, University of Uludag, Institute of Health Sciences) ;
  • Tunca, Berrin (Department of Medical Biology, University of Uludag, Institute of Health Sciences) ;
  • Egeli, Unal (Department of Medical Biology, University of Uludag, Institute of Health Sciences) ;
  • Tezcan, Gulcin (Department of Medical Biology, University of Uludag, Institute of Health Sciences) ;
  • Akyildiz, Elif Ulker (Department of Pathology, Uludag University Faculty of Medicine) ;
  • Bayram, Ahmet Sami (Department of Thoracıc Surgery, Uludag University Faculty of Medicine) ;
  • Gebitekin, Cengiz (Department of Thoracıc Surgery, Uludag University Faculty of Medicine) ;
  • Kurt, Ender (Department of Medical Oncology, Uludag University Faculty of Medicine) ;
  • Evrensel, Turkkan (Department of Medical Oncology, Uludag University Faculty of Medicine)
  • 발행 : 2014.04.30
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초록

Background: Thymomas and thymic carcinomas are rare malignancies and devising clinically effective molecular targeted therapies is a major clinical challenge. The aim of the study was to analyze BLC2 and vascular endothelial growth factor receptor (VEGFR) expression and KRAS and EGFR mutational status and to correlate them with the clinical characteristics of patients with thymomas and thymic carcinomas. Materials and Methods: A total of 62 patients (mean age: $50.4{\pm}13.2$ years) with thymomas and thymic carcinomas were enrolled. The expression of BLC2 and VEGFR in tumor cells and normal tissues was evaluated by RT-PCR. The mutational status of the KRAS and EGFR genes was investigated by PCR with sequence specific primers. Results: The BLC2 and VEGFR expression levels did not differ significantly between tumor and normal tissues. Moreover, there were no clearly pathogenic mutations in KRAS or EGFR genes in any tumor. None of the molecular markers were significantly related to clinical outcomes. Conclusions: Changes in levels of expression of BLC2 and VEGFR do not appear to be involved in thymic tumorigenesis. Moreover, our data suggest that KRAS and EGFR mutations do not play a major role in the pathogenesis of thymomas and thymic carcinomas.

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참고문헌

  1. Cao C, Sun SF, Lu D, et al (2013). Utility of VEGF and sVEGFR-1 in bronchoalveolar lavage fluid for differential diagnosis of primary lung cancer. Asian Pac J Cancer Prev, 14, 2443-6. https://doi.org/10.7314/APJCP.2013.14.4.2443
  2. Christodoulou C, Murray S, Dahabreh J, et al (2008). Response of malignant thymoma to erlotinib. Ann Oncol, 19, 1361-2. https://doi.org/10.1093/annonc/mdn388
  3. Cimpean AM, Raica M, Encica S, et al (2008). Immunohistochemical expression of vascular endothelial growth factor A (VEGF), and its receptors (VEGFR1, 2) in normal and pathologic conditions of the human thymus. Ann Anat, 190, 238-45. https://doi.org/10.1016/j.aanat.2007.05.003
  4. Cuddihy AR, Ge S, Zhu J, et al (2009). VEGF-mediated crosstalk within the neonatal murine thymus. Blood, 113, 2723-31. https://doi.org/10.1182/blood-2008-06-162040
  5. Detterbeck FC, Zeeshan A (2013). Thymoma: current diagnosis and treatment. Chin Med J (Engl), 126, 2186-91.
  6. Girard N (2010). Thymic tumors: relevant molecular data in the clinic. J Thorac Oncol, 5, 291-5. https://doi.org/10.1097/JTO.0b013e3181d39b56
  7. Kelly RJ (2013). Thymoma versus thymic carcinoma: differences in biology impacting treatment. J Natl Compr Canc Netw, 11, 577-83.
  8. Kim MJ, Woo SJ, Yoon CH, et al (2011). Involvement of autophagy in oncogenic K-Ras-induced malignant cell transformation. J Biol Chem, 286, 12924-32. https://doi.org/10.1074/jbc.M110.138958
  9. Kurup A, Burns M, Dropcho S, et al (2005). Phase II study of gefitinib treatment in advanced thymic malignancies. J Clin Oncol, 23, 7068.
  10. Lamarca A, Moreno V, Feliu J (2013). Thymoma and thymic carcinoma in the target therapies era. Cancer Treat Rev, 39, 413-20. https://doi.org/10.1016/j.ctrv.2012.11.005
  11. Li Q, Yin J, Wang X, et al (2013).B-cell Lymphoma 2 rs17757541 C>6 polymorphism was associated with on increased risk of gastric cardiac adenocarcinoma in a Chinese. Asian Pac J Cancer Prev, 14, 4301-6. https://doi.org/10.7314/APJCP.2013.14.7.4301
  12. Masaoka A (2010). Staging system of thymoma. J Thorac Oncol, 5, 304-12. https://doi.org/10.1097/JTO.0b013e3181f20c05
  13. Meister M, Schirmacher P, Dienemann H, et al (2007). Mutational status of the epidermal growth factor receptor (EGFR) gene in thymomas and thymic carcinomas. Cancer Lett, 248, 186-91. https://doi.org/10.1016/j.canlet.2006.07.003
  14. Mikhail M, Mekhail Y, Mekhail T (2012). Thymic neoplasms:a clinical update. Curr Oncol Rep, 14, 350-8. https://doi.org/10.1007/s11912-012-0246-8
  15. Ono M, Kuwano M (2006). Molecular mechanisms of epidermal growth factor receptor (EGFR) activation and response to gefitinib and other EGFR-targeting drugs. Clin Cancer Res, 12, 7242-51. https://doi.org/10.1158/1078-0432.CCR-06-0646
  16. Polo V, Girard N, Besse B (2013). Thymic tumours: an update. Presse Med, 42, 311-6. https://doi.org/10.1016/j.lpm.2013.07.002
  17. Rajan A, Giaccone G (2010). Targeted therapy for advanced thymic tumors. J Thorac Oncol, 5, 361-4. https://doi.org/10.1097/JTO.0b013e3181cee1ea
  18. Roma C, Esposito C, Rachiglio AM, et al (2013). Detection of EGFR mutations by taqman mutation detection assays powered by competitive allele-specific TaqMan PCR technology. Biomed Res Int, 2013, 385087.
  19. Sasaki H, Yukiue H, Kobayashi Y, et al (2001). Elevated serum vascular endothelial growth factor and basic fibroblast growth factor levels in patients with thymic epithelial neoplasms. Surg Today, 31, 1038-40. https://doi.org/10.1007/s005950170021
  20. Schubbert S, Shannon K, Bollag G (2007). Hyperactive Ras in developmental disorders and cancer. Nat Rev Cancer, 7, 295-308. https://doi.org/10.1038/nrc2109
  21. Sentman CL, Shutter JR, Hockenbery D, Kanagawa O, Korsmeyer SJ (1991). bcl-2 inhibits multiple forms of apoptosis but not negative selection in thymocytes. Cell, 67, 879-88. https://doi.org/10.1016/0092-8674(91)90361-2
  22. Strobel P, Hartmann M, Jakob A, et al (2004). Thymic carcinoma with overexpression of mutated KIT and the response to imatinib. N Engl J Med, 350, 2625-6. https://doi.org/10.1056/NEJM200406173502523
  23. Strobel P, Marx A, Zettl A, Muller-Hermelink HK (2005). Thymoma and thymic carcinoma: an update of the WHO Classification 2004. Surg Today, 35, 805-11. https://doi.org/10.1007/s00595-005-3047-y
  24. Venuta F, Rendina EA, Anile M, et al (2012). Thymoma and thymic carcinoma. Gen Thorac Cardiovasc Surg, 60, 1-12.
  25. Wang Y, Kaiser CE, Frett B, Li HY (2013). Targeting mutant KRAS for anticancer therapeutics: a review of novel small molecule modulators. J Med Chem, 56, 5219-30. https://doi.org/10.1021/jm3017706
  26. Yamaguchi H, Soda H, Kitazaki T, et al (2006). Thymic carcinoma with epidermal growth factor receptor gene mutations. Lung Cancer, 52, 261-2. https://doi.org/10.1016/j.lungcan.2006.01.009
  27. Yoh K, Nishiwaki Y, Ishii G, et al (2008). Mutational status of EGFR and KIT in thymoma and thymic carcinoma. Lung Cancer, 62, 316-20. https://doi.org/10.1016/j.lungcan.2008.03.013