Effects of fermented Taraxacum mongolicum by Dongchimi on protection of liver cell and immune activity

포공영(민들레)의 동치미 발효에 의한 간세포 보호 및 면역증진 효과

  • Choi, Bog-Soo (Gradute School Alternative Medicine, Kyonggi Univercity) ;
  • Kim, Hye-Ja (Gradute School Alternative Medicine, Kyonggi Univercity)
  • 최복수 (경기대학교 대체의학대학원) ;
  • 김혜자 (경기대학교 대체의학대학원)
  • Received : 2014.03.17
  • Accepted : 2014.04.17
  • Published : 2014.04.30

Abstract

Objective : This study set out to combine the treatment efficacy of Taraxacum with Dongchimi fermentation and investigate Taraxacum's effects on protection of liver cell and controlling nitric oxide(NO) through experiments, thus checking whether it had values as a physiological active matter. The experimental materials include Taraxacum Dongchimi (TD) and Taraxacum fermented by Dongchimi (TDF). As for methodology, experiments were carried out to compare TD and TDF in components, protection effects for liver cells, anticancer effects on liver cells, and protection effects for brain cells in the aspects of liver function and immunity enhancement. Method : The experimental materials include Taraxacum Dongchimi (TD) and Taraxacum fermented by Dongchimi (TDF). As for methodology, experiments were carried out to compare TD and TDF in components, protection effects for liver cells, anti-cancer effects on liver cells, and protection effects for brain cells in the aspects of liver function and immunity enhancement. Results : As shown in the chromatogram results, each valid component content increased in Taraxacum fermented by Dongchimi (TDF) for each time section. Of them, the valid component content at 36.80 minutes was approximately 2.7 times higher in TDF at 21.8% than in Taraxacum Dongchimi (TD) at 8.28%. TDF generated more excellent protection effects against the toxicity that caused oxidative damage to the liver cell(HepG2) with t-BHP than TD. The survival rate was low in TD of $100{\mu}g/m{\ell}$ and $300{\mu}g/m{\ell}$ and increased to 23.3% in TDF of $100{\mu}g/m{\ell}$. The survival rate was the highest at $300{\mu}M$ with a significant difference of 68.1%(P<0.05). Both TD and TDF showed effects of controlling nitric oxide production according to concentration with TDF recording a higher rate of controlling nitric oxide production than TD. There were significant differences(P<0.05) in the effects of controlling nitric oxide production at 200 ug/ml, 400 ug/ml in both groups. Especially the result TDF of $400{\mu}g/m{\ell}$ was thus similar to those of butein, the positive control group. Conclusion : The result of this studies is that Taraxacum fermented by Dongchimi (TDF) increased the valid component content compared with the simple mixture(TD). The findings clearly show that it is a material with the effects of improving immunity and liver cell protection. If fermentation methods are further developed to use it as a functional material, it will be subject to more opportunities of being used in other functional foods and make a contribution to integrated medicinal food development.

Keywords

References

  1. 심민교. 정화 임상본초학(精華 臨床本草學). 영림사. 2010; pp. 458
  2. Hyeong Woo Park, In Gun Park. Dandelion diffusion in western area of Kyunggi province. J ecology and environment. 1997; 11: 2-4
  3. Hee Don Choi, Yun Joung Koh, Yun Suk Kim, In Wuk Choi, Dong Soo Cha. Changes in Physiochemical and Sensory Characteristics of Dandelion(Taraxacum officinale) Leaves by Roasting Traatment. Korean J. Food Sci. Technol. 2007; 39: 515-520
  4. Seok Jin Kim. Fatty liver and probiotics. J Complement Altern Med Sci. 2012; 2:53-59
  5. 농림축산 식품부 식품 분석표. 농림축산부. 2012; pp. 2-4
  6. 성상권(양구토종 민들레영농조합법인). 민들레 건강법. 더어울림. 2010; pp 34-35, pp 89-99
  7. Kyung Min Kim. Study of biological activities in the fermented dandelion(Taraxacum officinale) extracts with raw sugar content. Department of biology graduate school of Kangwon university. 2012; pp. 2-7
  8. 문기원, 김혜자. Effects of Zymolysis-Diet Therapy on Intestinal Micro Flora and Autoimmune disease. J. Complement. Altern. Med. Sci., 2011; 1:25-40
  9. Seong Won Moon. Effect of Salt Concentration on Dongchimi Fermentation. Department of food and nutrition graduate school of Dankook university. 1995; pp. 5-12
  10. Jae Sik Park. The study for manufacture of quick fermented Dongchimi Department of animal science graduate school of Kangwon university. 2008; pp. 4-11
  11. Kyu Chan Hwang. Bacterial florae ageing fermented vegetables and productivity of vitamin B12. Department of food science graduate school of Kyunghee university. 1983; pp. 5-12
  12. Chung Kyu Oh. Effect of salt on probiotics isolated form Kimchi. Department of graduate school of Kyunghee university. 1986; pp. 5-15
  13. Sook Ja An. The effect of Salt and Food Preservatives on the Growth of Lactic acid bacteria isolated from Kimchi. Koran society of food and cookery science. 1988; 4: 39
  14. Ji Hye Ha, Myoung Hoon Jeong, Yong Chang Seo, Woon Yong Choi, Ji Seon Kim, Haeng Hoon Kim, Ju Hee Ahn, Hyeon Yong Lee. Enhancement of antioxidant activities of bark of Berberies koreana Palibin by lactic acid fermentation. Korean J Medical crop Sci. 2010;18: 421-428
  15. 차성관. 프로바이오틱 미생물과 유산균 식품. 미생 물산업, 한국미생물학회. 2000;26: 13-21
  16. 박병두, 김혜자. 유산균을 이용한 대두 발효 추출물이 면역계 활성에 미치는 영향. 대한예방한의학회지. 2012; 16: 139-153
  17. 강미정. 서영호. 김종배. 신승렬. 김광수. 한국산 서양민들레(Taraxacum officinale)의 화학적 조성. Krean J. Soc. Food Sci. 2000; 16: 2
  18. Kyung Hee Roh, Jin Joo Kim, Young Seon Song. Effect on liver protection of Dandelion extract. Korean society of food science and nutrition. 2007; 10: 1
  19. Choong Moo Park. Molecular mechanisms of anti-inflammation and hepatoprotection by Taraxacum officinale leaves. Department of food science graduate school of Injae university. 2011; pp. 21
  20. Do Youn Im, Kyoung-In Lee. Nitric Oxide Production Inhibitory and Scavenging Activity and Tyrosinase Inhibitory Activity of Extracts from Taraxacum officinale and Taraxacum coreanum. Korean J Medical Crop Sci. 2011; 19: 362-367 https://doi.org/10.7783/KJMCS.2011.19.5.362