Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Ramie Leaf according to Drying Methods on Antioxidant Activity and Growth Inhibitory Effects of Cancer Cells

건조방법에 따른 모시잎의 항산화 활성 및 암세포 증식 억제효과

  • Kim, Ah-Ra (Dept. of Food and Nutrition, Chosun University) ;
  • Kang, Su-Tae (Dept. of Food and Nutrition, Chosun University) ;
  • Jeong, Eun (Dept. of Food and Nutrition, Chosun University) ;
  • Lee, Jae-Joon (Dept. of Food and Nutrition, Chosun University)
  • 김아라 (조선대학교 식품영양학과) ;
  • 강수태 (조선대학교 식품영양학과) ;
  • 정은 (조선대학교 식품영양학과) ;
  • 이재준 (조선대학교 식품영양학과)
  • Received : 2014.01.14
  • Accepted : 2014.03.05
  • Published : 2014.05.31
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Abstract

This study was carried out to discriminate the effects of the ramie leaf according to the drying methods (hot air drying and freeze drying) on antioxidative activity in vitro and antiproliferation in human cancer cells. There were no significant differences in total polyphenol content of ramie leaf ethanol extracts depending on the drying methods, but total flavonoid content was significantly higher in hot air dried ramie leaf (HR) than in freeze dried ramie leaf (FR). The DPPH radical scavenging activity of HR and FR ethanol extracts were found to be 77.74%, and 77.29% in 1000 ppm, respectively. Antioxidative index of HR and FR ethanol extracts measured by Rancimat were lower than those in BHT, BHA, and ascorbic acid, but were higher than that in control. The antiproliferation effect of 80% ethanol extracts of HR and FR on liver cancer cell line (H460), stomach cancer cell line (AGS), and lung cancer cell line (A549) were increased with a dose-dependent manner. The cancer cell growth inhibition activities of HR and FR ethanol extracts at the concentration of $800{\mu}g/mL$ showed greater than 80% on Hep G2 and A549 cell line, and greater than 75% on AGS cell line. These results suggest that HR and FR ethanol extracts possess potential antioxidative effect and antiproliferation in human cancer cells, and those activities of ramie leaf ethanol extracts depending on the drying methods were similar.

본 연구는 모시잎의 건조방법에 따른 항산화 및 암세포 증식억제효과를 비교 분석하였다. 80% 에탄올로 추출한 열풍건조 및 동결건조 모시잎의 총 폴리페놀 함량은 건조방법에 따른 유의적 차이를 보이지 않았다. 반면 총 플라보노이드 함량은 열풍건조 모시잎이 동결건조 모시잎에 비하여 유의하게 높게 나타났다. 1,000 ppm에서 열풍건조 및 동결건조 모시잎의 DPPH 라디칼 소거능은 각각 77.74%와 77.29%의 비슷한 라디칼 소거능을 보였으며, Rancimat으로 측정한 열풍건조 및 동결건조 모시잎의 항산화지수는 각각 1.38과 1.32로 BHT, BHA 및 비타민 C에 비해서는 낮았으나 시료를 미첨가한 음성대조군보다는 높은 항산화력을 보였다. 위암(AGS), 간암(Hep G2) 및 폐암(A549) 세포에 대한 모시잎 에탄올 추출물의 암세포 증식 억제효과를 알아본 결과, 열풍건조 및 동결건조 모시잎 에탄올 추출물의 농도가 증가할수록 암세포의 증식 억제 활성은 농도 의존적으로 증가되었다. 건조방법에 따른 항암활성은 비슷한 경향이었으며 가장 높은 농도인 $800{\mu}g/mL$에서 80% 이상의 간암 및 폐암세포의 증식 억제 활성을 보였고, 위암세포에서는 75%이상의 증식 억제 활성을 보였다. 이상의 결과 열풍건조 및 동결건조 모시잎 에탄올 추출물은 in vitro에서 항산화효과와 암세포의 증식 억제효과를 보였고, 건조방법에 따른 모시잎 에탄올 추출물의 항산화 활성 및 암세포 증식 억제 활성은 비슷한 경향으로 나타났다.

Keywords

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