Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
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Investigation of Bordered Pit Ultrastructure in Tracheid of Korean Red Pine (Pinus densiflora) by Confocal Reflection Microscopy

공초점반사현미경을 이용한 소나무 유연벽공의 초미세구조 연구

  • Kwon, Ohkyung (National Instrumentation Center for Environmental Management, Seoul National University)
  • 권오경 (서울대학교 농생명과학공동기기원)
  • Received : 2014.04.08
  • Accepted : 2014.05.09
  • Published : 2014.05.25
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Abstract

Confocal reflection microscopy (CRM) was utilized to create 3-dimensional images of bordered pits and cell wall in the tracheid of Korean red pine (Pinus densiflora). Ultrastructures of torus, margo, and pit border were clearly observable in the CRM micrograph. Micrograph of cross-field pit revealed the connecting and supporting structure between tracheid and ray parenchyma cell. The CRM micrographs enabled to investigate detailed structures of tracheid cell wall such as S1, S2, S3 layers, transition layers between these layers, and microfibril (MF) orientation in S3 and S2 layers as well as complicated distribution of MF orientation around bordered pits. Not only concentric MF orientation of border thickening in the pit border was observed, but also changes in MF orientation from the cell wall to the border. From the experimental results, the CRM was thought to be a versatile microtechnique to investigate detailed structures of cell wall and bordered pit in the tracheid and cross-field pit between tracheid and ray parenchyma cell.

소나무의 가도관과 유연벽공의 미세구조를 연구하는데 공초점반사현미경법을 이용하여 획득한 3차원 화상을 사용하였다. 가도관 유연벽공의 토러스, 마르고, 벽공연의 미세구조가 명확하게 관찰되었으며, 교분야벽공의 미세구조로 가도관과 방사유세포 사이의 연결구조 및 방사유세포 내의 역학적 지지구조도 관찰할 수 있었다. 가도관 세포벽의 3차원 화상에서는 S1, S2, S3층과 이 층들의 사이에 있는 이행층의 존재도 확인할 수 있었다. 또한 S3층과 S2층의 마이크로 피브릴 배향의 관찰이 가능하였고, 유연벽공 주변의 복잡한 마이크로피브릴 배향 특성도 직접적으로 확인할 수 있었다. 본 연구의 결과 공초점반사현미경법은 소나무 가도관의 세포벽, 유연벽공, 교분야벽공의 미세구조를 연구하는데 유용하게 이용될 수 있는 현미경 기법으로 여겨졌다.

Keywords

References

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