Parasites, Hosts and Diseases

  • 제52권1호
  • /
  • Pages.21-26
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  • 2014
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Expression of Translationally Controlled Tumor Protein (TCTP) Gene of Dirofilaria immitis Guided by Transcriptomic Screening

  • Fu, Yan (Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University) ;
  • Lan, Jingchao (The Sichuan Key Laboratory for Conservation Biology on Endangered Wildlife, Developing Toward a State Key Laboratory for China, Chengdu Research Base of Giant Panda Breeding) ;
  • Wu, Xuhang (Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University) ;
  • Yang, Deying (Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University) ;
  • Zhang, Zhihe (The Sichuan Key Laboratory for Conservation Biology on Endangered Wildlife, Developing Toward a State Key Laboratory for China, Chengdu Research Base of Giant Panda Breeding) ;
  • Nie, Huaming (Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University) ;
  • Hou, Rong (The Sichuan Key Laboratory for Conservation Biology on Endangered Wildlife, Developing Toward a State Key Laboratory for China, Chengdu Research Base of Giant Panda Breeding) ;
  • Zhang, Runhui (Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University) ;
  • Zheng, Wanpeng (Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University) ;
  • Xie, Yue (Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University) ;
  • Yan, Ning (Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University) ;
  • Yang, Zhi (The Sichuan Key Laboratory for Conservation Biology on Endangered Wildlife, Developing Toward a State Key Laboratory for China, Chengdu Research Base of Giant Panda Breeding) ;
  • Wang, Chengdong (The Sichuan Key Laboratory for Conservation Biology on Endangered Wildlife, Developing Toward a State Key Laboratory for China, Chengdu Research Base of Giant Panda Breeding) ;
  • Luo, Li (The Sichuan Key Laboratory for Conservation Biology on Endangered Wildlife, Developing Toward a State Key Laboratory for China, Chengdu Research Base of Giant Panda Breeding) ;
  • Liu, Li (The Sichuan Key Laboratory for Conservation Biology on Endangered Wildlife, Developing Toward a State Key Laboratory for China, Chengdu Research Base of Giant Panda Breeding) ;
  • Gu, Xiaobin (Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University) ;
  • Wang, Shuxian (Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University) ;
  • Peng, Xuerong (Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University) ;
  • Yang, Guangyou (Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University)
  • 투고 : 2013.06.19
  • 심사 : 2013.11.12
  • 발행 : 2014.02.28
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초록

Dirofilaria immitis (heartworm) infections affect domestic dogs, cats, and various wild mammals with increasing incidence in temperate and tropical areas. More sensitive antibody detection methodologies are required to diagnose asymptomatic dirofilariasis with low worm burdens. Applying current transcriptomic technologies would be useful to discover potential diagnostic markers for D. immitis infection. A filarial homologue of the mammalian translationally controlled tumor protein (TCTP) was initially identified by screening the assembled transcriptome of D. immitis (DiTCTP). A BLAST analysis suggested that the DiTCTP gene shared the highest similarity with TCTP from Loa loa at protein level (97%). A histidine-tagged recombinant DiTCTP protein (rDiTCTP) of 40 kDa expressed in Escherichia coli BL21 (DE3) showed immunoreactivity with serum from a dog experimentally infected with heartworms. Localization studies illustrated the ubiquitous presence of rDiTCTP protein in the lateral hypodermal chords, dorsal hypodermal chord, muscle, intestine, and uterus in female adult worms. Further studies on D. immitis-derived TCTP are warranted to assess whether this filarial protein could be used for a diagnostic purpose.

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참고문헌

  1. Abraham D. Biology of Dirofilaria immitis. In Boreham, PFL, Atwell RB eds, Dirofilariasis. Boca Raton. CRC Press. 1988, p 29-46.
  2. Neiffer DL, Klein EC, Calle PP, Linn M, Terrell SP, Walker RL, Todd D, Vice CC, Marks SK. Mortality associated with melarsomine dihydrochloride administration in two North American river otters (Lontra canadensis) and a red panda (Ailurus fulgens fulgens). J Zoo Wildl Med 2002; 33: 242-248. https://doi.org/10.1638/1042-7260(2002)033[0242:MAWMDA]2.0.CO;2
  3. Riley SP, Foley J, Chomel B. Exposure to feline and canine pathogens in bobcats and gray foxes in urban and rural zones of a national park in California. J Wildl Dis 2004; 40: 11-22. https://doi.org/10.7589/0090-3558-40.1.11
  4. Ruiz de Ybanez MR, Martinez-Carrasco C, Martinez JJ, Ortiz JM, Attout T, Bain O. Dirofilaria immitis in an African lion (Panthera leo). Vet Rec 2006; 158: 240-242. https://doi.org/10.1136/vr.158.7.240
  5. Simon F, Morchon R, Gonzalez-Miguel J, Marcos-Atxutegi C, Siles-Lucas M. What is new about animal and human dirofilariosis? Trends Parasitol 2009; 25: 404-409. https://doi.org/10.1016/j.pt.2009.06.003
  6. McCall JW, Genchi C, Kramer LH, Guerrero J, Venco L. Heartworm disease in animals and humans. Adv Parasitol 2008; 66: 193-285. https://doi.org/10.1016/S0065-308X(08)00204-2
  7. Nelson CT, McCall JW, Rubin SB, Buzhardt LF, Dorion DW, Graham W, Longhofer SL, Guerrero J, Robertson-Plouch C, Paul A. 2005 Guidelines for the diagnosis, prevention and management of heartworm (Dirofilaria immitis) infection in dogs. Vet Parasitol 2005; 133: 255-266. https://doi.org/10.1016/j.vetpar.2005.07.008
  8. Böhm H, Benndorf R, Gaestel M, Gross B, Nürnberg P, Kraft R, Otto A, Bielka H. The growth-related protein P23 of the Ehrlich ascites tumor: translational control, cloning and primary structure. Biochem Int 1989; 19: 277.
  9. Chitpatima ST, Makrides S, Bandyopadhyay R, Brawerman G. Nucleotide sequence of a major messenger RNA for a 21 kilodalton polypeptide that is under translational control in mouse tumor cells. Nucleic Acids Res 1988; 16: 2350. https://doi.org/10.1093/nar/16.5.2350
  10. Yenofsky R, Cereghini S, Krowczynska A, Brawerman G. Regulation of mRNA utilization in mouse erythroleukemia cells induced to differentiate by exposure to dimethyl sulfoxide. Mol Cell Biol 1983; 3: 1197-1203. https://doi.org/10.1128/MCB.3.7.1197
  11. Bhisutthibhan J, Pan XQ, Hossler PA, Walker DJ, Yowell CA, Carlton J, Dame JB, Meshnick SR. The Plasmodium falciparum translationally controlled tumor protein homolog and its reaction with the antimalarial drug artemisinin. J Bio Chem 1998; 273: 16192-16198. https://doi.org/10.1074/jbc.273.26.16192
  12. Bini L, Heid H, Liberatori S, Geier G, Pallini V, Zwilling R. Twodimensional gel electrophoresis of Caenorhabditis elegans homogenates and identification of protein spots by microsequencing. Electrophoresis 1997; 18: 557-562. https://doi.org/10.1002/elps.1150180337
  13. Gnanasekar M, Rao KV, Chen L, Narayanan RB, Geetha M, Scott AL, Ramaswamy K, Kaliraj P. Molecular characterization of a calcium binding translationally controlled tumor protein homologue from the filarial parasites Brugia malayi and Wuchereria bancrofti. Mol Biochem Parasitol 2002; 121: 107-118. https://doi.org/10.1016/S0166-6851(02)00027-0
  14. Haghighat NG, Ruben L. Purification of novel calcium binding proteins from Trypanosoma brucei: properties of 22-, 24- and 38-kilodalton proteins. Mol Biochem Parasitol 1992; 51: 99-110. https://doi.org/10.1016/0166-6851(92)90205-X
  15. Walker DJ, Pitsch JL, Peng MM, Robinson BL, Peters W, Bhisutthibhan J, Meshnick SR. Mechanisms of artemisinin resistance in the rodent malaria pathogen Plasmodium yoelii. Antimicrob Agents Chemoth 2000; 44: 344-347. https://doi.org/10.1128/AAC.44.2.344-347.2000
  16. Yan F, Jingchao L, Zhihe Z, Rong H, Xuhang W, Deying Y. Novel Insights into the Transcriptome of Dirofilaria immitis. PLoS One 2012; 7: e41639. https://doi.org/10.1371/journal.pone.0041639
  17. Chen WJ, Niu DS, Zhang XY, Chen ML, Cui H, Wei WJ, Wen BH, Chen XR. Recombinant 56-kilodalton major outer membrane protein antigen of Orientia tsutsugamushi Shanxi and its antigenicity. Infect Immun 2003; 71: 4772-4779. https://doi.org/10.1128/IAI.71.8.4772-4779.2003
  18. Meyvis Y, Houthoofd W, Visser A, Borgonie G, Gevaert K, Vercruysse J, Claerebout E, Geldhof P. Analysis of the translationally controlled tumour protein in the nematodes Ostertagia ostertagi and Caenorhabditis elegans suggests a pivotal role in egg production. Int J Parasitol 2009; 39: 1205-1213. https://doi.org/10.1016/j.ijpara.2009.02.017
  19. MacDonald SM, Bhisutthibhan J, Shapiro TA, Rogerson SJ, Taylor TE, Tembo M, Langdon JM, Meshnick SR. Immune mimicry in malaria: Plasmodium falciparum secretes a functional histaminereleasing factor homolog in vitro and in vivo. Proc Natl Acad Sci USA 2001; 98: 10829-10832. https://doi.org/10.1073/pnas.201191498
  20. Rao KV, Chen L, Gnanasekar M, Ramaswamy K. Cloning and characterization of a calcium-binding, histamine-releasing protein from Schistosoma mansoni. J Biol Chem 2002; 277: 31207-31213. https://doi.org/10.1074/jbc.M204114200

피인용 문헌

  1. Development of Real-time PCR Assays for Detection of Dirofilaria immitis from Infected Dog Blood vol.48, pp.2, 2014, https://doi.org/10.15324/kjcls.2016.48.2.88
  2. Development of TaqMan Quantitative PCR Assays for Duplex Detection of Dirofilaria immitis COI and Dog GAPDH from Infected Dog Blood vol.51, pp.1, 2019, https://doi.org/10.15324/kjcls.2019.51.1.64
  3. Brown Spider (Loxosceles) Venom Toxins as Potential Biotools for the Development of Novel Therapeutics vol.11, pp.6, 2014, https://doi.org/10.3390/toxins11060355