Parasites, Hosts and Diseases

  • 제52권2호
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  • Pages.201-204
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  • 2014
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Metagonimus yokogawai: a 100-kDa Somatic Antigen Commonly Reacting with Other Trematodes

  • Han, Eun-Taek (Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University) ;
  • Yang, Hyun-Jong (Department of Parasitology, College of Medicine, Ewha Womans University) ;
  • Park, Young-Jin (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul National University) ;
  • Park, Jeong-Hyun (Department of Anatomy, School of Medicine, Kangwon National University) ;
  • Chai, Jong-Yil (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul National University)
  • 투고 : 2014.01.19
  • 심사 : 2014.03.12
  • 발행 : 2014.04.30
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초록

This study was undertaken to characterize the properties of a 100 kDa somatic antigen from Metagonimus yokogawai. Monoclonal antibodies (mAbs) were produced against this 100 kDa antigen, and their immunoreactivity was assessed by western blot analysis with patients' sera. The mAbs against the 100 kDa antigen commonly reacted with various kinds of trematode antigens, including intestinal (Gymnophalloides seoi), lung (Paragonimus westermani), and liver flukes (Clonorchis sinensis and Fasciola hepatica). However, this mAb showed no cross-reactions with other helminth parasites, including nematodes and cestodes. To determine the topographic distribution of the 100 kDa antigen in worm sections, indirect immunoperoxidase staining was performed. A strong positive reaction was observed in the tegumental and subtegumental layers of adult M. yokogawai and C. sinensis. The results showed that the 100 kDa somatic protein of M. yokogawai is a common antigen which recognizes a target epitope present over the tegumental layer of different trematode species.

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참고문헌

  1. Chai JY, Huh S, Yu JR, Kook J, Jung KC, Park EC, Sohn WM, Hong ST, Lee SH. An epidemiological study of metagonimiasis along the upper reaches of the Namhan River. Korean J Parasitol 1993; 31: 99-108. https://doi.org/10.3347/kjp.1993.31.2.99
  2. Chai JY, Murrell KD, Lymbery AJ. Fish-borne parasitic zoonoses: status and issues. Int J Parasitol 2005; 35: 1233-1254. https://doi.org/10.1016/j.ijpara.2005.07.013
  3. Chai JY, Shin EH, Lee SH, Rim HJ. Foodborne intestinal flukes in Southeast Asia. Korean J Parasitol 2009; 47(suppl): S69-S102. https://doi.org/10.3347/kjp.2009.47.S.S69
  4. Chai JY, Cho SY, Seo BS. Study on Metagonimus yokogawai (Katsurada, 1912) in Korea. IV. An epidemiological investigation along Tamjin River basin, South Cholla Do, Korea. Korean J Parasitol 1977; 151: 115-120.
  5. Chai JY, Lee SH. Food-borne intestinal trematode infections in the Republic of Korea. Parasitol Int 2002; 51: 129-154. https://doi.org/10.1016/S1383-5769(02)00008-9
  6. Chai JY, Yu JR, Lee SH, Jung HC, Song IS, Cho SY. An egg-negative patient of acute metagonimiasis diagnosed serologically by ELISA. Seoul J Med 1989; 30: 139-142.
  7. Johansen MV, Sithithaworn P, Bergquist R, Utzinger J. Towards improved diagnosis of zoonotic trematode infections in Southeast Asia. Adv Parasitol 2010; 73: 171-260. https://doi.org/10.1016/S0065-308X(10)73007-4
  8. Anuracpreeda P, Wanichanon C, Chawengkirtikul R, Chaithirayanon K, Sobhon P. Fasciola gigantica: immunodiagnosis of fasciolosis by detection of circulating 28.5 kDa tegumental antigen. Exp Parasitol 2009; 123: 334-340. https://doi.org/10.1016/j.exppara.2009.08.014
  9. Liu F, Cui SJ, Hu W, Feng Z, Wang ZQ, Han ZG. Excretory/secretory proteome of the adult developmental stage of human blood fluke, Schistosoma japonicum. Mol Cell Proteomics 2009; 8: 1236-1251. https://doi.org/10.1074/mcp.M800538-MCP200
  10. Gaudier JF, Cabán-Hernández K, Osuna A, Espino AM. Biochemical characterization and differential expression of a 16.5-kilodalton tegument-associated antigen from the liver fluke Fasciola hepatica. Clin Vaccine Immunol 2012; 19: 325-333. https://doi.org/10.1128/CVI.05501-11
  11. Song CY, Dresden MH. Partial purification and characterization of cysteine proteinases from various developmental stages of Paragonimus westermani. Comp Biochem Physiol 1990; 95: 473-476.
  12. Song CY, Dresden MH, Rege AA. Clonorchis sinensis purification and characterization of a cysteine proteinase from adult worms. Comp Biochem Physiol 1990; 97: 825-829.
  13. Li Y, Huang Y, Hu X, Liu X, Ma C, Zhao J, Wu Z, Xu J, Yu X. 41.5-kDa cathepsin L protease from Clonorchis sinensis: expression, characterization, and serological reactivity of one excretory-secretory antigen. Parasitol Res 2012; 111: 673-680. https://doi.org/10.1007/s00436-012-2885-y
  14. Kueakhai P, Changklungmoa N, Chaithirayanon K, Songkoomkrong S, Riengrojpitak S, Sobhon P. Production and characterization of a monoclonal antibody against recombinant saposin-like protein 2 of Fasciola gigantica. Acta Trop 2013; 125: 157-162. https://doi.org/10.1016/j.actatropica.2012.11.001
  15. Coles GC, Rubano D. Antigenicity of a proteolytic enzyme of Fasciola hepatica. J Helminthol 1988; 62: 257-260. https://doi.org/10.1017/S0022149X00011627
  16. Ahn H, Rim HJ, Kim SJ. Antigenic localities in the tissues of Metagonimus yokogawai observed by immunogold labeling method. Korean J Parasitol 1991; 29: 245-257. https://doi.org/10.3347/kjp.1991.29.3.245
  17. Anuracpreeda P, Wanichanon C, Chaithirayanon K, Preyavichyapugdee N, Sobhon P. Distribution of 28.5 kDa antigen in the tegument of adult Fasciola gigantica. Acta Trop 2006; 100: 31-40. https://doi.org/10.1016/j.actatropica.2006.09.006
  18. Mulvenna J, Sripa B, Brindley PJ, Gorman J, Jones MK, Colgrave ML, Jones A, Nawaratna S, Laha T, Suttiprapa S, Smout MJ, Loukas A. The secreted and surface proteomes of the adult stage of the carcinogenic human liver fluke Opisthorchis viverrini. Proteomics 2010; 10: 1063-1078.
  19. Chai JY, Han ET, Park YK, Guk SM, Kim JL, Lee SH. High endemicity of Metagonimus yokogawai infection among residents of Samchok-shi, Kangwon-do, Korea. Korean J Parasitol 2000; 38: 33-36. https://doi.org/10.3347/kjp.2000.38.1.33
  20. Lowry OH, Rosenbrough NJ, Lewis FA, Randall RJ. Protein measurements with folin phenol reagent. J Biol Chem 1951; 193: 265-275.
  21. Hanna RE. Fasciola hepatica: glycocalyx replacement in the juvenile as a possible mechanism for protection against host immunity. Exp Parasitol 1980; 50: 103-114. https://doi.org/10.1016/0014-4894(80)90012-0
  22. Lee SC, Chung YB, Kong Y, Kang SY, Cho SY. Antigenic protein fractions of Metagonimus yokogawai reacting with patient sera. Korean J Parasitol 1993; 31: 43-48. https://doi.org/10.3347/kjp.1993.31.1.43

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