한국수정란이식학회지 (Journal of Embryo Transfer)

  • 제29권1호
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  • Pages.91-99
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  • 2014
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  • 2508-755X(pISSN)
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  • 2288-0178(eISSN)
한국수정란이식학회 (The Korean Society of Embryo Transfer)
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Scanning Electrochemical Microscopy를 이용한 한우 체내 수정란의 호흡률 조사

Respiration Rates of Individual Bovine In Vivo-Produced Embryos Measured with a Novel, Scanning Electrochemical Microscopy

  • 김현 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 복난희 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 김성우 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 도윤정 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 김민규 (충남대학교 동물자원생명과학과) ;
  • 조상래 (농촌진흥청 국립축산과학원 난지축산시험장) ;
  • 성환후 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 김동훈 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 고응규 (농촌진흥청 국립축산과학원 가축유전자원시험장)
  • Kim, Hyun (Animal Genetic Resources Station, National Institute of Animal Science, R.D.A.) ;
  • Bok, Nan-Hee (Animal Genetic Resources Station, National Institute of Animal Science, R.D.A.) ;
  • Kim, Sung-Woo (Animal Genetic Resources Station, National Institute of Animal Science, R.D.A.) ;
  • Do, Yoon-Jung (Animal Genetic Resources Station, National Institute of Animal Science, R.D.A.) ;
  • Kim, Min-Kyu (Department of Animal Science, Graduate School of Chungnam National University) ;
  • Cho, Sang-Rae (Subtropical Genetics Resources Station, National Institute of Animal Science, R.D.A.) ;
  • Seong, Hwan-Hoo (Animal Genetic Resources Station, National Institute of Animal Science, R.D.A.) ;
  • Kim, Dong Hun (Animal Genetic Resources Station, National Institute of Animal Science, R.D.A.) ;
  • Ko, Yeoung-Gyu (Animal Genetic Resources Station, National Institute of Animal Science, R.D.A.)
  • 투고 : 2014.01.07
  • 심사 : 2014.03.25
  • 발행 : 2014.03.31
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초록

Oxygen consumption is a useful parameter for evaluating mammalian embryo quality, since individual bovine embryos was noninvasively quantified by scanning electrochemical microscopy (SECM). Recently, several approaches have been used to measure the oxygen consumption rates of individual embryos, but relationship between oxygen consumption and pregnancy rates of Hanwoo following embryo transfer has not yet been reported. In this study, we measured to investigate the correlation between oxygen consumption rate and pregnancy rates of Hanwoo embryo using a SECM. In addition to, the expression of pluripotent gene and anti-oxidant enzyme was determined using real-time PCR by extracting RNA according to the oxygen consumption of in vivo embryo. First, we found that the oxygen consumption significantly increased in blastocyst-stage embryos (blastocyst) compared to early blastocyst stage embryos, indicating that oxygen consumption reflects the embryo quality (Grade I). Oxygen consumption of blastocyst was measured using a SECM and total cell number of in vitro blastocyst was enumerated by counting cells stained by propidium iodide. The oxygen consumption or GI blastocysts were significantly higher than those of GII blastocysts ($10.2{\times}10^{15}/mols^{-1}$ versus $6.4{\times}10^{15}/mols^{-1}$, p<0.05). Total cell numbers of in vitro blastocysts were 74.8, 90.7 and 110.2 in the oxygen consumption of below 10.0, 10.0~12.0 and over $12.0{\sim}10^{15}/mols^{-1}$, respectively. Pregnant rate in recipient cow was 0, 60 and 80% in the transplantation of embryo with the oxygen consumption of below 10.0, 10.0~12.0 and over $12.0{\times}10^{15}/mols^{-1}$, respectively. GPX1 and SOD1 were significantly increased in over -10.0 group than below 10.0 groups but in catalase gene, there was no significant difference. On the other hand, In OCT-4 and Sox2, pluripotent gene, there was a significant difference (p<0.05) between the below-10.0 ($0.98{\pm}0.1$) and over 10.0 ($1.79{\pm}0.2$). In conclusion, these results suggest that measurement of oxygen consumption maybe help increase the pregnant rate of Hanwoo embryos.

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참고문헌

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