DOI QR코드

DOI QR Code

Toxicity Evaluation of Burkholderia pyrrocinia CAB08106-4 in Cyprinus carpio and Daphnia magna

Burkholderia pyrrocinia CAB08106-4 원제가 잉어 및 물벼룩에 미치는 영향 연구

  • Cho, Jae-Gu (Hoseo Toxicological Research Center (HTRC), Hoseo University) ;
  • Kim, Mee-Seon (Hoseo Toxicological Research Center (HTRC), Hoseo University) ;
  • Choi, Hyun-Jung (Hoseo Toxicological Research Center (HTRC), Hoseo University) ;
  • Kwon, Min (Hoseo Toxicological Research Center (HTRC), Hoseo University) ;
  • Kang, Tae-Gu (Hoseo Toxicological Research Center (HTRC), Hoseo University) ;
  • Chung, Chang-Kook (Agricultural Research Center, Hankook Samgong) ;
  • Kim, Kyun (Hoseo Toxicological Research Center (HTRC), Hoseo University) ;
  • Oh, Seung-Min (Hoseo Toxicological Research Center (HTRC), Hoseo University) ;
  • Park, Cheol-Beom (Hoseo Toxicological Research Center (HTRC), Hoseo University)
  • 조재구 (호서대학교 안전성평가센터) ;
  • 김미선 (호서대학교 안전성평가센터) ;
  • 최현정 (호서대학교 안전성평가센터) ;
  • 권민 (호서대학교 안전성평가센터) ;
  • 강태구 (호서대학교 안전성평가센터) ;
  • 정창국 (한국삼공 연구소) ;
  • 김균 (호서대학교 안전성평가센터) ;
  • 오승민 (호서대학교 안전성평가센터) ;
  • 박철범 (호서대학교 안전성평가센터)
  • Received : 2014.01.08
  • Accepted : 2014.02.28
  • Published : 2014.03.31

Abstract

Bukholderia pyrrocinia CAB08106-4 has an anti-fungal effect on Garlic White Rot caused by Sclereotium cepivorum and Sclereotium sp. It is environmentally friendly microbial product that prevents and controls a variety of phytopathogens including Garlic White Rot caused by Sclereotium cepivorum and Sclereotium sp. The aim of this study was to assess the environmental toxicity using Cyprinus carpio and Daphnia magna. Bukholderia pyrrocinia CAB08106-4 ($1.0{\times}10^9cfu/mL$) was adminatrated to Cyprinus carpio and Daphnia magna according to the toxicity test guideline for peciticide. $LC_{50}$ of Bukholderia pyrrocinia CAB08106-4 is over $6.67{\times}10^4cfu/mL$ in Cyprinus carpio and Daphnia magna and no adverse effect was observed. Based on these results, we concluded that Bukholderia pyrrocinia CAB08106-4 has no toxiciy for Cyprinus carpio and Daphnia magna.

Burkholderia pyrrocinia CAB08106-4은 Sclereotium cepivorum과 Sclereotium sp. 균주에 의해 발생하는 마늘 흑색 썩음균핵병에 대한 항박테리아 효능을 가지고 있다. 이는 Sclereotium cepivorum과 Sclereotium sp.에 의해 발생하는 마늘 흑색썩음 균핵병을 포함한 다양한 식물병원체를 관리하고 예방하기 위하여 개발중인 친환경적인 미생물 제품이다. 본 연구에서는 마늘 흑색 썩음균핵병의 방제재로 개발중인 Bukholderia pyrrocinia CAB08106-4 원제($1.0{\times}10^9cfu/mL$)가 잉어와 물벼룩에 미치는 영향을 평가하였으며, 그 결과 $6.67{\times}10^4cfu/mL$의 농도에서는 독성이 관찰되지 않았다. 따라서 Burkholderia pyrrocinia CAB08106-4 원제는 담수어류 및 담수무척추동물에 영향이 낮을 것으로 기대된다.

Keywords

References

  1. Baudouin, M. F. and P. Scoppa (1974) Acute toxicity of various metals to fresh water zooplankton. Bull. Environ. Contam. Toxicol. 12:745-751. https://doi.org/10.1007/BF01685925
  2. British Crop Protection Council (2014) book shop reference http://www.bcpc.org/shop/The-Manual-of-Biocontrol-Agents-Fourth-edition.html Accessed 01 April 2014.
  3. Guidelines for safety evaluation of microbial pesticides (Draft translation). No.9-5090, Japan's MAFF, 1997.
  4. Han, K. S., B. R. Kim, J. T. Kim, S. S. Hahm, C. K. Chung, Y. G. Nam, S. H. Yu and J. E, Choi (2013) Biological control of white rot in garlic using burkholderia pyrrocinia CAB0106-4. Res. Plant Dis. 19(1):21-24. https://doi.org/10.5423/RPD.2013.19.1.021
  5. Kim, Y. K. (2005) Ecology and control strategy of white rot of garlic and onion. Rural Development Administration Cooperative Research Report, 6-12.
  6. Kwon, M., T. K. Kang, C. K. Chung and C. B. Park. (2013) Acute oral, pulmonary and intravenous toxicity/pathogenicity testing of burkholderia pyrrochinia CAB08106-4 of in rats. Korean J. Pestic. Sci. 17(3):193-199. https://doi.org/10.7585/kjps.2013.17.3.193
  7. Mancebo, A., T. Molier, B. Gonzalez, S. Lugo, L. Riera, M. E. Arteaga, A. M. Bada, Y. Gonzalez, M. Pupo, Y. Hernandez, C. Gonzalez, N. M. Rojas, and G. Rodriguez (2011) Acute oral, pulmonary and intravenous toxicity/pathogenicity testing of a new formulation of Bacilus thuringiensis var israelensis SH-14 in rats. Regul. Toxicol. Pharm. 59:184-190. https://doi.org/10.1016/j.yrtph.2010.10.003
  8. Park, K. S. (2011) Development of Biopesticide and Role of Bacillus spp. KIC News, 14(4):1-11.
  9. Rural Development Administration (2012). Notisfication No. 2012-37. 2012 RDA. Korea.
  10. Siegel, J. P., J. A. Shadduck and J. Szabo (1987) Safety of the entomopathogen Bacillus thuringiensis var. israselensis for mammals. J. Econ. Entomol. 80:717-723. https://doi.org/10.1093/jee/80.4.717
  11. Sprague, J. B. (1969) Measurement of pollutant toxicity to fish. I Bioassay methods for acute toxicity. Water Res. 3:793-821. https://doi.org/10.1016/0043-1354(69)90050-5
  12. Whalon, M. E. and B. A. Wingerd (2003) Bt: mode of action and use. Arch. Insect Biochem. Physiol. 54:200-211. https://doi.org/10.1002/arch.10117
  13. Yu, S. H., Y. S. Kim, H. S. Cho, M. S. Ko, N. Y. Lee, H. M. Lee, M. K. Cho and C. Chang (2005) Development of biofungicide for control of powdery mildews. Rural technology development report. 1-124.