디지털융복합연구 (Journal of Digital Convergence)

  • 제12권3호
  • /
  • Pages.359-366
  • /
  • 2014
  • /
  • 2713-6434(pISSN)
  • /
  • 2713-6442(eISSN)
한국디지털정책학회 (The Society of Digital Policy and Management)
권호 표지
DOI QR코드

DOI QR Code

도라지 추출물의 항균작용 및 항염작용

Antibacterial and anti-inflammatory effects of Platycodon grandiflorum extracts

  • 김정 (수원여자대학교 치위생과)
  • Kim, Jung (Dept. of Dental Hygiene, Suwon Women's University)
  • 투고 : 2014.02.10
  • 심사 : 2014.03.20
  • 발행 : 2014.03.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구에서는 도라지 추출물의 항균활성을 밝히고자 S. mutans와 C. albicans에 대한 영향을 조사하였다. 또한 항염활성을 알아보고자 Raw 264.7 세포에서 추출물 농도에 따른 NO 생성 저해율을 조사하였다. 연구 재료는 국내산 100% 도라지분말을 구입하여 사용하였으며, 실험균주로는 S. mutans KCTC 3065 와 C. albicans KCTC 7965를 사용하여 다음과 같은 결과를 얻었다. 도라지 추출물의 농도가 높을수록 S. mutans의 성장 억제율이 현저히 높아졌다. 도라지 추출물의 농도가 높을수록 C. albicans의 성장 억제율이 현저히 높아졌다. Raw 264.7세포에서 추출물을 10, $20{\mu}g/ml$의 농도로 첨가하였을 때 각각 29.2, 26.1%의 NO 생성 저해율이 나타났다. 이는 도라지가 항균 및 항염물질로서 활용 가능함을 의미한다.

This study investigated the impact on S. mutans와 C. albicans in order to reveal the antimicrobial activity of Platycodon grandiflorum extracts. In addition, it examined NO generation inhibition rate in accordance with the extract concentration from Raw 264.7 cell in order to find out anti-inflammatory activation. As for the study materials, domestically made 100% Platycodon grandiflorum powder was utilized. As for the experimental strain, S. mutans KCTC 3065 and C. albicalns KCTC 7965 were utilized. Consequently, this study obtained the following results. Growth inhibition rate of S. mutans became significantly higher with higher concentration of Platycodon grandiflorum extracts. Growth inhibition rate of C. albicans became significantly higher with higher concentration of Platycodon grandiflorum extracts. NO generation inhibition rate was found to be 29.2% and 26.1% respectively when adding Platycodon grandiflorum extract with the concentration of 10 and $20{\mu}g/ml$ in Raw 264.7 cells. These results mean that Platycodon grandiflorum could be leveraged as antimicrobial and anti-inflammatory substance.

키워드

참고문헌

  1. Hanada S, Slade HD, Biology, immunology, and cariogenicity of Streptococcus mutans, Microbiol Rev, 35, 331-384, 1990.
  2. Choi HD, Koh YJ, Choi IW, Kim YS, Pack YK. Anticariogenic activity and glucosyltransferase inhibitory effects of extracts from pine needle and twing, Korean J Food Sci Technol 39, 336-341, 2007.
  3. Lee YS, Park HJ, You JS, Park HH, Kwon IB, Lee HY, Isolation of an anticariogenic compound from magnoliae bark, Korean J Food Sci Technol 30, 230-236, 1998.
  4. Park CS, Shin YS, Ryu IW, Lee KS, Antimicrobial activity of extracts from aloevera peel against Streptococcus mutans JC-2 (I), Korean J Food Nutr 13, 139-145, 2000.
  5. Nikawa H, Iwanaga H, Kameda M, Hamada T, In vitro evaluation of Candida albicans adherence to soft denture-lining materials. J Prosthet Dent 68(5), 804-808, 1992. https://doi.org/10.1016/0022-3913(92)90206-P
  6. Kim MJ, Shin SW, Lee JY, In vitro study on the adherence and penetration of Candida albicans into denture soft lining materials, Journal of the Korean academy of prosthodontic society 44(4), 466-476, 2006.
  7. Holing JF, Anibal PC, Obando-Pereda GA, Peixoto IA, Furletti VF, Foglio MA, Gonclves RB, Antimicrobial potential of some plant extracts against Candida species, Braz J Biol 70(4), 1065-1068, 2010. https://doi.org/10.1590/S1519-69842010000500022
  8. Santos VR, Pimenta FJ, Aguiar MC, do Carmo MA, Naves MD, Mesquita RA, Oral candidiasis treatment with Brazilian ethanol propolis extract, Phytother Res 19(7), 652-654, 2005. https://doi.org/10.1002/ptr.1715
  9. Choung MG, Sohn EH, Anti-tumor Activity of saponin fraction of Platycodon grandiflorum through immunomodulatory effects associated with NO production in RAW 264.7 cells. Korean J. Plant Res. 24, 557-563, 2011. https://doi.org/10.7732/kjpr.2011.24.5.557
  10. Shon MY, Seo JK, Kim HJ, Sung NJ, Chemical compositions and physiological activities of Doraji (Platycodon grandiflorum), J. Korean Soc. Food Sci. Nutr. 30, 717-720, 2001.
  11. Choi CY, J. Y. Kim, Y.S. Kim, Y. C. Chung, J. K. Seo and H.G. Jeong, 2001. Aqueous extract isolated from Platycodon grandiflorum elicits the release of nitric oxide and tumor necrosis factor-${\alpha}$ from murine macrophages, Int. Immunopharmacol. 1, 1141-1151, 2001. https://doi.org/10.1016/S1567-5769(01)00047-9
  12. Choi HD, Koh YJ, Choi IW, Kim YS, Pack YK, Anticariogenic activity and glucosyltransferase inhibitory effects of extracts from pine needle and twing. Korean JFood Sci Technol 39, 336-341, 2007.
  13. Lee HO, Lee KH, Park NK, Jeong SI, B SH, Han DM, Antibacterial effects of sophora flavescens on Streptococcus mutans, Korean J Food Nutr 13, 536-546, 2000.
  14. Choi SC, Juns JS, Studies of antimicrobial from extracts of impatiens balsamina (I). Korean J Fiber Soc 34, 393-399, 1997.
  15. Kim OM, Ha DJ, Jeong YJ, Antibacterial activity of vinegars on Streptococcus mutans caused dental caries, Korean J Food Preserv 10, 565-569, 2003.
  16. Chandra J, Kuhn DM, Mukherjee PKHoyer LL, McCORMICK T, Ghannoum MA, Biofilm formation by the fungal pathogen Candida albicans: Development, Architecture and Drug Resistance, J. Bacteriol 183(18), 5385-5394, 2001. https://doi.org/10.1128/JB.183.18.5385-5394.2001
  17. Kim SY, Lee EB, Jeong EJ, Anti-inflammatory action of the fractions of platycodi radix, J Korean Soc Food Sci Nutr 22(4), 618-624, 2009.
  18. Choung MG and Sohn EH, Anti-tumor activity of saponin fraction of Platycodon grandiflorum through immunomodulatory effects associated with NO prodution in RAW 264.7 cells, Kor.J. Plant Res. 24(5) 557-563, 2011 https://doi.org/10.7732/kjpr.2011.24.5.557
  19. Jang, J. R., Hwang, S. Y. and Lim, S. Y. Inhibitory effect of extracts of Platycodon grandiflorum (the Ballon Flower) on oxidation and nitric oxide production. Korean J Food Preserv 18, 65-71, 2011. https://doi.org/10.11002/kjfp.2011.18.1.065
  20. Yu-Jin Ko, Hui-Gyeong Seol, Gyeong-Ran Lee, Gye-Im Jeong, Chung-Ho Ryu, Anti-inflammatory Effect and Antioxidative Activities of Ingredients used in Bibimbab, J Life Sci 23(2), 213-221, 2013. https://doi.org/10.5352/JLS.2013.23.2.213