Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Direct ROS Scavenging Activity of CueP from Salmonella enterica serovar Typhimurium

  • Yoon, Bo-Young (College of Pharmacy and Research Institute for Drug Development, Pusan National University) ;
  • Yeom, Ji-Hyun (Department of Life Science, Chung-Ang University) ;
  • Kim, Jin-Sik (College of Pharmacy and Research Institute for Drug Development, Pusan National University) ;
  • Um, Si-Hyeon (College of Pharmacy and Research Institute for Drug Development, Pusan National University) ;
  • Jo, Inseong (College of Pharmacy and Research Institute for Drug Development, Pusan National University) ;
  • Lee, Kangseok (Department of Life Science, Chung-Ang University) ;
  • Kim, Yong-Hak (Department of Microbiology, Catholic University of Daegu, School of Medicine) ;
  • Ha, Nam-Chul (College of Pharmacy and Research Institute for Drug Development, Pusan National University)
  • Received : 2013.08.27
  • Accepted : 2013.12.18
  • Published : 2014.02.28
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Abstract

Salmonella enterica serovar Typhimurium (S. Typhimurium) is an intracellular pathogen that has evolved to survive in the phagosome of macrophages. The periplasmic copper-binding protein CueP was initially known to confer copper resistance to S. Typhimurium. Crystal structure and biochemical studies on CueP revealed a putative copper binding site surrounded by the conserved cysteine and histidine residues. A recent study reported that CueP supplies copper ions to periplasmic Cu,Zn-superoxide dismutase (SodCII) at a low copper concentration and thus enables the sustained SodCII activity in the periplasm. In this study, we investigated the role of CueP in copper resistance at a high copper concentration. We observed that the survival of a cueP-deleted strain of Salmonella in macrophage phagosome was significantly reduced. Subsequent biochemical experiments revealed that CueP specifically mediates the reduction of copper ion using electrons released during the formation of the disulfide bond. We observed that the copper ion-mediated Fenton reaction in the presence of hydrogen peroxide was blocked by CueP. This study provides insight into how CueP confers copper resistance to S. Typhimurium in copper-rich environments such as the phagosome of macrophages.

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References

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