Fisheries and Aquatic Sciences

한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)
권호 표지
DOI QR코드

DOI QR Code

Anti-inflammatory Effect of an Ethanolic Extract of Myagropsis yendoi in Lipopolysaccharide-Stimulated BV-2 Microglia Cells

  • Salih, Sarmad Ali (KOICA-PKNU International Graduate Program of Fisheries Sciences, Pukyong National University) ;
  • Kim, Hyeung-Rak (Department of Food Science and Nutrition, Pukyong National University)
  • 투고 : 2013.11.19
  • 심사 : 2014.01.23
  • 발행 : 2014.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Marine brown algae have been identified as a rich source of structurally diverse bioactive compounds. Whether Myagropsis yendoi ethanolic extracts (MYE) inhibit inflammatory responses was investigated using lipopolysaccharide (LPS)-stimulated microglia BV-2 cells. MYE inhibited LPS-induced nitric oxide (NO) production in a dose-dependent manner and suppressed the expression of inducible nitric oxide synthase in BV-2 cells. MYE also reduced the production of pro-inflammatory cytokines in LPS-stimulated BV-2 cells. LPS-induced nuclear factor-${\kappa}B$ (NF-${\kappa}B$) transcriptional activity and NF-${\kappa}B$ translocation into the nucleus were significantly inhibited by MYE treatment through preventing degradation of the inhibitor ${\kappa}B-{\alpha}$. Moreover, MYE inhibited the phosphorylation of AKT, ERK, JNK, and p38 mitogen-activated protein kinase in LPS-stimulated BV-2 cells. These results indicate that MYE is a potential source of therapeutic or functional agents for neuroinflammatory diseases.

키워드

참고문헌

  1. Bozinovski S, Jones JE, Vlahos R, Hamilton JA and Anderson GP. 2002. Granulocyte/macrophage-colony-stimulating factor (GM-CSF) regulates lung innate immunity to lipopolysaccharide through Akt/Erk activation of NF-${\kappa}B$ and AP-1 in vivo. J Biol Chem 277, 42808-42814. http://dx.doi.org/10.1074/jbc.M207840200.
  2. Brown GC and Neher JJ. 2010. Inflammatory neurodegeneration and mechanisms of microglial killing of neurons. Mol Neurobiol 41, 242-247. http://dx.doi.org/10.1007/s12035-010-8105-9.
  3. Cantley LC. 2002. The phosphoinositide 3-kinase pathway. Science 296, 1655-1657. http://dx.doi.org/10.1126/science.296.5573.1655.
  4. Cunningham C. 2012. Microglia and neurodegeneration: the role of systemic inflammation. Glia 61, 71-90. http://dx.doi.org/10.1002/glia.22350.
  5. Gwon WG, Lee MS, Kim JS, Kim, JI, Lim CW, Kim NG and Kim HR. 2013. Hexane fraction from Sargassum fulvellum inhibits lipopolysaccharide-induced inducible nitric oxide synthase expression in RAW 264.7 cells via NF-${\kappa}B$ pathways. Am J Chin Med 41, 565-584. http://dx.doi.org/10.1142/S0192415X13500407 .
  6. Herlaar E and Brown Z. 1999. p38 MAPK signalling cascades in inflammatory disease. Mol Med Today 5, 439-447. http://dx.doi.org/10.1016/S1357-4310(99)01544-0.
  7. Jana M, Jana A, Liu X, Ghosh S and Pahan K. 2007. Involvement of phosphatidylinositol 3-kinase-mediated up-regulation of $I{\kappa}B{\alpha}$ in anti-inflammatory effect of gemfibrozil in microglia. J Immunol 179, 4142-4152. https://doi.org/10.4049/jimmunol.179.6.4142
  8. Jang BC, Paik JH, Kim SP, Shin DH, Song DK, Park JG, Suh MH, Park JW and Suh SI. 2005. Catalase induced expression of inflammatory mediators via activation of NF-${\kappa}B$, PI3K/AKT, p70S6K, and JNKs in BV2 microglia. Cell Signal 17, 625-633. http://dx.doi.org/10.1016/j.cellsig.2004.10.001.
  9. Janssen-Heininger YMW, Poynter ME and Baeuerle PA. 2000. Recent advances towards understanding redox mechanisms in the activation of nuclear factor kappaB. Free Radic Biol Med 28, 1317-1327. http://dx.doi.org/10.1016/S0891-5849(00)00218-5.
  10. Joung EJ, Lee MS, Choi JW, Kim JS, Shin T, Jung BM, Kim JI and Kim HR. 2012a. Anti-inflammatory effects of phlorofucofuroeckol B-rich ethyl acetate fraction obtained from Myagropsis myagroides on lipopolysaccharide-stimulated RAW 264.7 cells and mouse edema. Int Immunopharmacol 14, 471-480. http://dx.doi.org/10.1016/j.intimp.2012.08.021.
  11. Joung EJ, Lee MS, Choi JW, Kim JS, Shin T, Jung BM, Yoon NY, Lim CW, Kim JI and Kim HR. 2012b. Anti-inflammatory effect of ethanolic extract from Myagropsis myagroides on murine macrophages and mouse ear edema. BMC Complement Altern Med 12, 171. http://dx.doi.org/10.1186/1472-6882-12-171.
  12. Jung WK, Lee DY, Park C, Choi YH, Choi I, Park SG, Seo SK, Lee SW, Yea SS, Ahn SC, Lee CM, Park WS, Ko JH and Choi IW. 2010. Cilostazol is anti-inflammatory in BV2 microglial cells by inactivating nuclear factor-kappaB and inhibiting mitogen-activated protein kinases. Br J Pharmacol 159, 1274-1285. http://dx.doi.org/10.1111/j.1476-5381.2009.00615.x.
  13. Kang GJ, Han SC, Yoon WJ, Koh YS, Hyun JW, Kang HK, Youl Cho J and Yoo ES. 2013. Sargaquinoic acid isolated from Sargassum siliquastrum inhibits lipopolysaccharide-induced nitric oxide production in macrophages via modulation of nuclear factor-${\kappa}B$ and c-Jun N-terminal kinase pathways. Immunopharmacol Immunotoxicol 35, 80-87. http://dx.doi.org/10.3109/08923973.2012.698622.
  14. Kim AR, Lee, MS, Shin TS, Hua H, Jang BC, Choi JS, Byun DS, Utsuki T, Ingram D and Kim HR. 2011. Phlorofucofuroeckol A inhibits the LPS-stimulated iNOS and COX-2 expressions in macrophages via inhibition of NF-${\kappa}B$, Akt, and p38 MAPK. Toxicol in Vitro 25, 1789-1795. http://dx.doi.org/10.1016/j.tiv.2011.09.012.
  15. Kim AR, Lee MS, Choi JW, Utsuki T, Kim JI, Jang BC and Kim HR. 2013a. Phlorofucofuroeckol A suppresses expression of inducible nitric oxide synthase, cyclooxygenase-2, and pro-inflammatory cytokines via inhibition of nuclear factor-${\kappa}B$, c-Jun NH2-terminal kinases, and Akt in microglial cells. Inflammation 36, 259-271. http://dx.doi.org/10.1007/s10753-012-9542-6.
  16. Kim H, Lee HS, Chang KT, Ko TH, Baek KJ and Kwon NS. 1995. Chloromethyl ketones block induction of nitric oxide synthase in murine macrophages by preventing activation of nuclear factor-kappa B. J Immunol 154, 4741-4748.
  17. Kim S, Kim JI, Choi JW, Kim, M, Yoon NY, Choi CG, Choi JS and Kim HR. 2013b. Anti-inflammatory effect of hexane fraction from Myagropsis myagroides ethanolic extract in lipopolysaccharide-stimulated BV-2 microglial cells. J Pharm Pharmacol 65, 895-906. http://dx.doi.org/10.1111/jphp.12049.
  18. Kim SN, Choi HY, Lee W, Park GM, Shin WS and Kim YK. 2008. Sargaquinoic acid and sargahydroquinoic acid from Sargassum yezoense stimulate adipocyte differentiation through $PPAR{\alpha}/{\gamma}$ activation in 3T3-L1 cells. FEBS Lett 582, 3465-3472. http://dx.doi.org/10.1016/j.febslet.2008.09.011.
  19. Lee MS, Kwon MS, Choi JW, Shin T, No HK, Choi JS, Byun DS, Kim JI and Kim HR. 2012. Anti-inflammatory activities of an ethanol extract of Ecklonia stolonifera in lipopolysaccharide-stimulated RAW 264.7 murine macrophage cells. J. Agric Food Chem 60, 9120-9129. http://dx.doi.org/10.1021/jf3022018.
  20. Makarov SS. 2001. NF-kappa B in rheumatoid arthritis: a pivotal regulator of inflammation, hyperplasia, and tissue destruction. Arthritis Res 3, 200-206. https://doi.org/10.1186/ar300
  21. Marks-Konczalik J, Chu SC and Moss J. 1998. Cytokine-mediated transcriptional induction of the human inducible nitric oxide synthase gene requires both activator protein 1 and nuclear factor ${\kappa}B$-binding sites. J Biol Chem 273, 22201-22208. https://doi.org/10.1074/jbc.273.35.22201
  22. Meda L, Cassatella MA, Szendrei GI, Otvos L, Baron P, Villalba M, Ferrari D and Rossi F. 1995. Activation of microglial cells by ${\beta}$-amyloid protein and interferon-${\gamma}$. Nature 374, 647-650. http://dx.doi.org/10.1038/374647a0.
  23. Minghetti L, Walsh DT, Levi G and Perry VH. 1999. In vivo expression of cyclooxygenase-2 in rat brain following intraparenchymal injection of bacterial endotoxin and inflammatory cytokines. J Neuropathol Exp Neurol 58, 1184-1191. http://dx.doi.org/10.1097/00005072-199911000-00008.
  24. Oh YT, Lee JY, Lee J, Kim H, Yoon KS, Choe W and Kang I. 2009. Oleic acid reduces lipopolysaccharide-induced expression of iNOS and COX-2 in BV2 murine microglial cells: possible involvement of reactive oxygen species, p38 MAPK, and IKK/NF-${\kappa}B$ signaling pathways. Neurosci Lett 464, 93-97. http://dx.doi.org/10.1016/j.neulet.2009.08.040.
  25. Packard RRS and Libby P. 2008. Inflammation in atherosclerosis: from vascular biology to biomarker discovery and risk prediction. Clin Chem 54, 24-38. http://dx.doi.org/10.1373/clinchem.2007.097360.
  26. Pahl HL. 1999. Activators and target genes of Rel/NF-${\kappa}B$ transcription factors. Oncogene 18, 6853-6866. http://dx.doi.org/ 10.1038/sj.onc.1203239.
  27. Silva AR, Pinheiro AM, Souza CS, Freitas SRVB, Vasconcellos V, Freire SM, Velozo ES, Tardy M, El-Bacha RS, Costa MFD and Costa SL. 2008. The flavonoid rutin induces astrocyte and microglia activation and regulates TNF-alpha and NO release in primary glial cell cultures. Cell Biol Toxicol 24, 75-86. http://dx.doi.org/10.1007/s10565-007-9017-y.
  28. Skaper SD. 2007. The brain as a target for inflammatory processes and neuroprotective strategies. Ann N Y Acad Sci 1122, 23-34. http://dx.doi.org/10.1196/annals.1403.002.
  29. Teismann P, Tieu K, Cohen O, Choi DK, Wu DC, Marks D, Vila M, Jackson-Lewis V and Przedborski S. 2003. Pathogenic role of glial cells in Parkinson's disease. Mov Disord 18, 121-129. http://dx.doi.org/10.1002/mds.10332.
  30. Xie Q, Whisnant R and Nathan C. 1993. Promoter of the mouse gene encoding calcium-independent nitric oxide synthase confers inducibility by interferon gamma and bacterial lipopolysaccharide. J Exp Med 1776, 1779-1784. http://dx.doi.org/10.1084/jem.177.6.1779.
  31. Yang EJ, Ham YM, Yang KW, Lee NH and Hyun CG. 2013. Sargachromenol from Sargassum micracanthum inhibits the lipopolysaccharide-induced production of inflammatory mediators in RAW 264.7 macrophages. ScientificWorldJournal. Article ID 712303. http://dx.doi.org/10.1155/2013/712303.