Journal of Ginseng Research

  • 제38권2호
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  • Pages.116-122
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  • 2014
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  • 1226-8453(pISSN)
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  • 2093-4947(eISSN)
고려인삼학회 (The Korean Society of Ginseng)
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Re-evaluation of physicochemical and NMR data of triol ginsenosides Re, Rf, Rg2, and 20-gluco-Rf from Panax ginseng roots

  • Cho, Jin-Gyeong (Graduate School of Biotechnology and Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • In, Seo-Ji (Graduate School of Biotechnology and Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Jung, Ye-Jin (Graduate School of Biotechnology and Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Cha, Byeong-Ju (Graduate School of Biotechnology and Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Lee, Dae-Young (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Kim, Yong-Bum (Technology Services Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Yeom, Myeonghun (Amorepacific Corporation Research and Development Center) ;
  • Baek, Nam-In (Graduate School of Biotechnology and Department of Oriental Medicinal Materials and Processing, Kyung Hee University)
  • 투고 : 2013.08.31
  • 심사 : 2013.12.22
  • 발행 : 2014.04.15
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초록

Ginseng roots were extracted with aqueous methanol, and extracts were suspended in water and extracted successively with ethyl acetate and n-butanol. Column chromatography using the n-butanol fraction yielded four purified triol ginseng saponins: the ginsenosides Re, Rf, Rg2, and 20-gluco-Rf. The physicochemical, spectroscopic, and chromatographic characteristics of the ginsenosides were measured and compared with reports from the literature. For spectroscopic analysis, two-dimensional nuclear magnetic resonance (NMR) methods such as $^1H$-$^1H$ correlation spectroscopy, nuclear Overhauser effect spectroscopy, heteronuclear single quantum correlation, and heteronuclear multiple bond connectivity were employed to identify exact peak assignments. Some peak assignments for previously published $^1H$-and $^{13}C$-NMR spectra were found to be inaccurate. This study reports the complete NMR assignment of 20-gluco-Rf for the first time.

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참고문헌

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  2. Simultaneous Quantitative Analysis of Ginsenosides Isolated from the Fruit of Panax ginseng C.A. Meyer and Regulation of HO-1 Expression through EGFR Signaling Has Anti-Inflammatory and Osteogenic Ind vol.26, pp.7, 2014, https://doi.org/10.3390/molecules26072092
  3. Physical, chemical, and biological characterization of ginsenoside F1 incorporated in nanostructured lipid carrier vol.45, pp.8, 2021, https://doi.org/10.1111/jfbc.13860