DOI QR코드

DOI QR Code

A New Record of Euonymus hamiltonianus Wall. in Dokdo: Using Molecular Markers of Nuclear and Plastid DNA

독도의 미기록 식물 참빗살나무: 핵과 엽록체 DNA의 분자마커 이용

  • Received : 2013.11.11
  • Accepted : 2014.03.21
  • Published : 2014.03.31

Abstract

The present scientific scenario shows that, the long-distance dispersal of any species plays a major role in population as well as the flow of individual between populations. However, newly recorded plant at Dokdo was confirmed by using morphological and molecular markers of nrDNA and cpDNA. This scientific study has identified the plant species was as a Euonymus hamiltonianus Wall. (Celastraceae). And after careful studies, the laboratory results shows that the distribution of tree species at Dokdo as an Oceanic Island is a meaningful thing because of the inflow of plant in adverse condition of environment such as a shallow soil layers and steep terrain. So it is necessary to continuous monitor for the inflow of non-indigenous plant into Dokdo.

종의 장거리 산포는 집단의 확산과 집단간 개체의 흐름에 중요한 역할을 한다. 본 연구는 독도에서 발견된 식물을 형태학적 특징과 핵 및 엽록체 DNA의 분자마커를 이용하여 종을 식별한 결과, 참빗살나무 (노박덩굴과)로 확인되었다. 얕은 토양층과 험준한 지형 등 입지적으로 열악한 대양섬인 독도에서 목본의 분포는 의미있는 결과이며, 향후 외부유입종의 지속적인 모니터링이 요구된다.

Keywords

References

  1. Bortiri E, SH Oh, J Jiang, S Baggett, A Granger, C Weeks, M Buckingham, D Potter and DE Parfitt. 2001. Phylogeny and Systematics of Prunus (Rosaceae) as Determined by Sequence Analysis of ITS and the Chloroplast trnL-trnF Spacer DNA. Syst. Bot. 26:797-807.
  2. CBOL Plant Working Group. 2009. A DNA barcode for land plants. Proc. Nat. Acad. Sci. 106:12794-12797. https://doi.org/10.1073/pnas.0905845106
  3. Denslow JS, JC Space and PA Thomas. 2009. Invasive exotic plants in the tropical pacific Islands: Patterns of diversity. Biotropica 41:162-170. https://doi.org/10.1111/j.1744-7429.2008.00469.x
  4. Doyle JJ and JL Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19:11-15.
  5. Dulloo ME, SP Kell and CG Jones. 2002. Impact and control of invasive alien species on small islands. Int. Forest. Rev. 4:277-285. https://doi.org/10.1505/ifor.4.4.277.40525
  6. Felsenstein J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783-791. https://doi.org/10.2307/2408678
  7. Geneious version (version 6.1.6) created by Biomatters. Available from http://www.geneious.com/.
  8. Howe HF and J Smallwood. 1982. Ecology of seed dispersal. Ann. Rev. Ecol. Syst. 13:201-228. https://doi.org/10.1146/annurev.es.13.110182.001221
  9. Huston MA. 2004. Management strategies for plant invasions: manipulating productivity, disturbance, and competition. Diversity Distrib. 10:167-178. https://doi.org/10.1111/j.1366-9516.2004.00083.x
  10. Hyun JO and SK Kwon. 2006. Flora of Dokdo. In Report on the detailed survey of Dokdo ecosystem, pp. 47-59. Ministry of Environment, Seoul. (in Korean)
  11. Kil JH, SH Park and KS Koh. 2006. The distribution of nonnative plants in Ulleung Island. Korean J. Plant Res. 19:237- 242. (in Korean)
  12. Kim HS and JG Oh. 2010. Distribution of naturalized plants in Dadohae National Marine Park. Korean. J. Plant Res. 23: 187-196.
  13. Kim HJ, BC Shin and MY Jeung. 2009. Research trend of Dokdo island plants - At focus of research data from 1947 to 2009. J. Korean Inst. Trad. Landscape Architecture 27:61-78. (in Korean)
  14. Kim JH and YS Kim. 1994a. A taxonomic study of Korean Euonymus L. (Celastraceae) based on the morphology of vegetative characters. Korean J. Plant Taxon. 24:215-230. (in Korean) https://doi.org/10.11110/kjpt.1994.24.4.215
  15. Kim JH and YS Kim. 1994b. A taxonomic study of Korean Euonymus L. (Celastraceae) based on the morphology of reproductive characters. Korean J. Plant Taxon. 24: 231-245. (in Korean) https://doi.org/10.11110/kjpt.1994.24.4.231
  16. Kim YS. 1998. Plant ecosystem of Ulleung and Dokdo islands. A Synthetic Research of Ulleung and Dokdo islands, pp. 621-678. Yeungnam University Institute of Korean Culture, Gyeongsan. (in Korean)
  17. Kim YS, HT Shin and KH Kang. 2000. Vascular Plants and Conservation of Rare and Endangered Plants in Ulnung Island. J. Resour. Dev. 19:13-30. (in Korean)
  18. Kimura MA. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16:111-120. https://doi.org/10.1007/BF01731581
  19. Lee DB and SU Joo. 1958. Reinvestigation of the flora of the Dagelet Island. Collection of dissertations for Liberal Arts and Sciences of Korea University 3:223-296. (in Korean)
  20. Lee DH, SH Cho and JH Pak. 2007. The analysis of vascular plant species composition in Dok-do Island. Korean J. Plant Taxon. 37:545-563. (in Korean) https://doi.org/10.11110/kjpt.2007.37.4.545
  21. Lee IG. 1978. The ecological consideration on the flora of Liancourt Island. Nat. Conserv. 22:20-23. (in Korean)
  22. Lee TB. 1978. Vegetaion of the Island Dok-to. Nat. Conserv. 22:16-19. (in Korean)
  23. Lee TB. 1980. Illustrated Flora of Korea. Hyangmunsa, Seoul. (in Korean)
  24. Lee TB. 2003. Coloured Flora of Korea. Hyangmunsa, Seoul. (in Korean)
  25. Lee WT. 1996. Standard illustrations of Korean Plants. Academy Publishing Co., Ltd, Seoul. (in Korean)
  26. Lee WT and IS Yang. 1981. The flora of Ulreung Is. and Dogdo Island. In A report on the scientific survey of the Ulreung and Dogdo Islands. The Korean Ass. Conserv. Nat. 19:61-93. (in Korean)
  27. Li HT, JB Yang, DZ Li, M Moller and A Shah. 2010. A molecular phylogenetic study of Hemsley (Cucurbitaceae) based on ITS, rpl16, trnH-psbA, and trnL DNA sequences. Plant Syst. Evol. 285:23-32. https://doi.org/10.1007/s00606-009-0252-y
  28. Li WP, FS Yang, T Jivkova and GS Yin. 2012. Phylogenetic relationships and generic delimitation of Eurasian Aster (Asteraceae: Astereae) inferred from ITS, ETS and trnL-F sequence data. Ann. Bot. 109:1341-1357. https://doi.org/10.1093/aob/mcs054
  29. Losos JB and CE Parent. 2010. The speciation-area relationship, pp. 415-438. In The theory of island biogeography revisited, Lolos JB and RE Ricklefs (eds.). Princeton University Press, Princeton and Oxford, USA and England.
  30. Miller SE. 2007. DNA barcoding and the renaissance of taxonomy. Proc. Nat. Acad. Sci. 104:4775-4776. https://doi.org/10.1073/pnas.0700466104
  31. Moller M and QCB Cronk. 1997. Origin and relationships of Saintpaulia (Gesneriaceae) based on ribosomal DNA internal transcribed spacer (ITS) sequences. Am. J. Bot. 84: 956-965. https://doi.org/10.2307/2446286
  32. Mu XY, LC Zhao and ZX Zhang. 2012. Phylogeny of Celastrus L. (Celastraceae) inferred from two nuclear and three plastid markers. J. Plant Res. 125:619-630. https://doi.org/10.1007/s10265-012-0484-8
  33. Nathan R, FM Schrr, O Spiegel, O Steinitz, A Trakhtenbrot and A Tsoar. 2008. Mechanisms of long-distance seed dispersal. Trends Ecol. Evol. 23:638-647. https://doi.org/10.1016/j.tree.2008.08.003
  34. Park SH, JH Shin, YM Lee, JH Lim and JS Moon. 2002. Distributions of naturalized alien plants in Korea. Korea Forest Research Institute & Korea National Arbortum. Ukgo Press, Seoul. (in Korean)
  35. Park SJ, IG Song, SJ Park and DO Lim. 2010. The flora and vegetation of Dokdo Island in Ulleung-gun, Gyeongsangbuk- do. Korean J. Environ. Ecol. 24:264-278. (in Korean)
  36. Park SJ, IG Song, SJ Park and JG Jin. 2011. Flora of Dokdo. In Ecosystem of Dokdo. Daegu Regional Environmental Office, Daegu. (in Korean)
  37. Shin HT and YS Kim. 2002. The Establishment of Conservation Area and Conservation Strategy in Ulleung Island (I)-Flora. Korean J. Environ. Ecol. 16:195-216. (in Korean)
  38. Simmons MP, JJ Cappa, RH Archer, AJ Ford, D Eichstedt and CC Clevinger. 2008. Phylogeny of the Celastreae (Celastraceae) and the relationships of Catha edulis (qat) inferred from morphological characters and nuclear and plastid genes. Mol. Phylogenet. Evol. 48:745-757. https://doi.org/10.1016/j.ympev.2008.04.039
  39. Simmons MP, MJ McKenna, CD Bacon, K Yakobson, JJ Cappa, RH Archer and AJ Ford. 2012. Phylogeny of Celastraceae tribe Euonymeae inferred from morphological characters and nuclear and plastid genes. Mol. Phylogenet. Evol. 62: 9-20. https://doi.org/10.1016/j.ympev.2011.08.022
  40. Song IG and SJ Park. 2012. Distribution and management of non-indigenous plants in Dokdo. Korean J. Plant Taxon. 42: 98-107. (in Korean) https://doi.org/10.11110/kjpt.2012.42.1.098
  41. Sun BY, MR Sul, JA Im, CH Kim and TJ Kim. 2002. Evolution of endemic vascular plants of Ulleungdo and Dokdo in Korea - floristic and cytotaxonomic characteristics of vascular flora of Dokdo. Korean J. Plant Taxon. 32:143-158. (in Korean) https://doi.org/10.11110/kjpt.2002.32.2.143
  42. Swofford DL. 2003. PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4. Sinauer Associates, Sunderland, Massachusetts, USA.
  43. Taberlet P, L Gielly, G Pautou and J Bouvet. 1991. Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol. Biol. 17, 1105-1109. https://doi.org/10.1007/BF00037152
  44. Thompson JD, TJ Gibson, F Plewniak, F Jeanmougin and DD Higgins. 1997. The clustal X windows interface : Flexible strategies for multiple sequence alignment aided by quality analysis tool. Nucleic Acids Res. 25:4876-4882. https://doi.org/10.1093/nar/25.24.4876
  45. Vijayan K and CH Tsou. 2010. DNA barcoding in plants: taxonomy in a new perspective. Current Sci. 99:1530-1541.
  46. White TJ, T Bruns, S Lee and J Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR protocols: A guide to methods and applications, pp. 315-322. Academic Press, San Diego, USA.
  47. Yu M, G Hu, KJ Feeley, J Wu and P Ding. 2012. Richness and composition of plants and birds on land-bridge islands: effects of island attributes and differential responses of species groups. J. Biogeogr. 39:1124-1133. https://doi.org/10.1111/j.1365-2699.2011.02676.x