Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Identification of salt and drought inducible glutathione S-transferase genes of hybrid poplar

  • Kwon, Soon-Ho (College of Life Science and Biotechnology, Korea University) ;
  • Kwon, Hye-Kyoung (Institute of Life Science and Natural Resources, College of Life Science and Biotechnology, Korea University) ;
  • Kim, Wook (College of Life Science and Biotechnology, Korea University) ;
  • Noh, Eun Woon (Division of Forest Biotechnology, Korea Forest Research Institute) ;
  • Kwon, Mi (College of Life Science and Biotechnology, Korea University) ;
  • Choi, Young Im (Division of Forest Biotechnology, Korea Forest Research Institute)
  • Received : 2013.11.25
  • Accepted : 2014.01.29
  • Published : 2014.03.31
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Abstract

Recent genome annotation revealed that Populus trichocarpa contains 81 glutathione S-transferase (GST) genes. GST genes play important and varying roles in plants, including conferring tolerance to various abiotic stresses. Little information is available on the relationship - if any - between drought/salt stresses and GSTs in woody plants. In this study, we screened the PatgGST genes in hybrid poplar (Populus alba ${\times}$ Populus tremula var. glandulosa) that were predicted to confer drought tolerance based on our expression analysis of all members of the poplar GST superfamily following exposure to salt (NaCl) and drought (PEG) stresses, respectively. Exposure to the salt stress resulted in the induction of eight PatgGST genes and down-regulation of one PatgGST gene, and the level of induction/repression was different in leaf and stem tissues. In contrast, 16 PatgGST genes were induced following exposure to the drought (PEG) stress, and two were down-regulated. Taken together, we identified seven PatgGSTs (PatgGSTU15, PatgGSTU18, PatgGSTU22, PatgGSTU27, PatgGSTU46, PatgGSTU51 and PatgGSTU52) as putative drought tolerance genes based on their induction by both salt and drought stresses.

Keywords

References

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