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Enhancement of ${\beta}$-Glucan Content in the Cultivation of Cauliflower Mushroom (Sparassis latifolia) by Elicitation

  • 투고 : 2013.10.01
  • 심사 : 2014.01.06
  • 발행 : 2014.03.31

초록

The effectiveness of three kinds of enzymes (chitinase, ${\beta}$-glucuronidase, and lysing enzyme complex), employed as elicitors to enhance the ${\beta}$-glucan content in the sawdust-based cultivation of cauliflower mushroom (Sparassis latifolia), was examined. The elicitors were applied to the cauliflower mushroom after primordium formation, by spraying the enzyme solutions at three different levels on the sawdust-based medium. Mycelial growth was fully accomplished by the treatments, but the metabolic process during the growth of fruiting bodies was affected. The application of a lysing enzyme resulted in an increase in the ${\beta}$-glucan concentration by up to 31% compared to that of the control. However, the treatment resulted in a decrease in mushroom yield, which necessitated the need to evaluate its economic efficiency. Although we still need to develop a more efficient way for using elicitors to enhance functional metabolites in mushroom cultivation, the results indicate that the elicitation technique can be applied in the cultivation of medicinal/edible mushrooms.

키워드

참고문헌

  1. Radman R, Saez T, Bucke C, Keshavarz T. Elicitation of plants and microbial cell systems. Biotechnol Appl Biochem 2003;37(Pt 1):91-102. https://doi.org/10.1042/BA20020118
  2. Asilonu E, Bucke C, Keshavarz T. Enhancement of chrysogenin production in cultures of Penicillium chrysogenum by uronic acid oligosaccharides. Biotechnol Lett 2000;22:931-6. https://doi.org/10.1023/A:1005694415666
  3. Han JR, Gao PP. Effect of several elicitors on sclerotia biomass and carotenoid yield of Penicillium sp. PT95 during solid-state fermentation of corn meal. World J Microbiol Biotechnol 2002;18:367-71.
  4. Murphy T, Roy I, Harrop A, Dixon K, Keshavarz T. Effect of oligosaccharide elicitors on bacitracin A production and evidence of transcriptional level control. J Biotechnol 2007; 131:397-403. https://doi.org/10.1016/j.jbiotec.2007.07.943
  5. Vanhulle S, Radman R, Parra R, Cui T, Bols CM, Tron T, Sannia G, Keshavarz T. Effect of mannan oligosaccharide elicitor and ferulic acid on enhancement of laccases production in liquid cultures of basidiomycetes. Enzyme Microb Technol 2007;40:1712-8. https://doi.org/10.1016/j.enzmictec.2006.10.002
  6. Zhu LW, Zhong JJ, Tang YJ. Significance of fungal elicitors on the production of ganoderic acid and Ganoderma polysaccharides by the submerged culture of medicinal mushroom Ganoderma lucidum. Process Biochem 2008;43: 1359-70. https://doi.org/10.1016/j.procbio.2008.08.003
  7. Park H, Lee BH, Oh DS, Ka KH, Bak WC, Lee HJ. Cultivation of cauliflower mushroom (Sparassis crispa) using coniferous sawdust-based media with barley flours. J Korean For Energy 2005;24:31-6.
  8. Park H, Ryu SR, Ka KH. Cultivation of Sparassis crispa on several kinds of medium density and particle size of sawdustbased medium made of Larix kaempferi. Mokchae Konghak 2011;39:68-74.
  9. Ryoo R, Sou HD, Ka KH, Park H. Phylogenetic relationships of Korean Sparassis latifolia based on morphological and ITS rDNA characteristics. J Microbiol 2013;51:43-8. https://doi.org/10.1007/s12275-013-2503-4
  10. Ohno N, Miura NN, Nakajima M, Yadomae T. Antitumor 1, 3-$\beta$-glucan from cultured fruit body of Sparassis crispa. Biol Pharm Bull 2000;23:866-72. https://doi.org/10.1248/bpb.23.866
  11. Harada T, Miura N, Adachi Y, Nakajima M, Yadomae T, Ohno N. Effect of SCG, 1,3-$\beta$-D-glucan from Sparassis crispa on the hematopoietic response in cyclophoshamide induced leukopenic mice. Biol Pharm Bull 2002;27:931-9.
  12. Ryu SR, Ka KH, Park H, Bak WC, Lee BH. Cultivation characteristics of Sparassis crispa strains using sawdust medium of Larix kaempferi. Kor J Mycol 2009;37:49-54. https://doi.org/10.4489/KJM.2009.37.1.049
  13. Carlile MJ, Watkinson SC. The fungi. London: Academic Press; 1994.
  14. Yun JH, Kim JH, Hwang YS, Byun SY, Kim DI. Taxol production in Taxus cell cultures: effects of various elicitors. Korean J Biotechnol Bioeng 1995;10:143-8.