Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Screening of Plant Extracts for Nemastatic Activity

선충 억제 활성 식물 추출물의 선발

  • Received : 2014.02.11
  • Accepted : 2014.03.20
  • Published : 2014.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

A total of 2,714 methanol extracts of 1,677 plant species in 178 families were screened for nemastatic activity against Rhabditis sp. by 96-well microplate bioassay. The plant extracts with a concentration of $5,000{\mu}g/ml$ were mixed with aqueous nematode solution containing about 20 Rhabditis sp. and their activity was examined daily for 7 days. Out of 2,714 plant extracts examined in this test, 2,362 (87.0%) showed no negative influence on the nematode activity, while 187 (6.9%) inhibited nematode activity about 50%, 95 (3.5%) inhibited nematode activity over 90%, and 70 (2.6%) rather enhanced nematode activity. Among those showing over 50% nemastatic activity, 25 extracts were randomly selected for further screening with Rhabditis sp. and with juveniles of Meloidogyne incognita. The screening revealed that 11 extracts (44%) were consistently nemastatic. The 282 extracts with nemastatic activity could be of use in future studies on nematicidal plants.

식물 기원의 살선충 물질 탐색 연구의 일환으로, 178과, 1,677종 식물의 부위별 조추출물 2,714종을 이용하여 이들이 선충의 활성에 미치는 영향을 검토하였다. 선충억제 활성 검정은 96-well microplate를 이용하였으며 부식성선충 20마리를 조추출물 $5,000{\mu}g/ml$에 침지하고 7일간 매일 관찰하였다. 시험결과 2,362종(87.0%)의 조추출물은 부식성선충에 전혀 영향을 미치지 않았고, 187종(6.9%)의 조추출물은 부식성선충의 활성을 50% 정도 억제하였고, 95종(3.5%)의 조추출물은 90% 이상 억제를 보였으며, 70종(2.6%)의 조추출물에서는 오히려 부식성선충이 번식되었다. 1차 검정에서 50% 이상 선충억제 활성을 보인 추출물중에서, 25개의 추출물을 임의로 선택하여 2, 3차(부식성선충), 4차(고구마뿌리혹선충 유충) 반복 시험을 수행한 결과, 약 44%의 시료는 지속적인 선충억제 활성을 보였다. 이번 시험에서 선충억제 활성을 나타낸 282개의 식물 조추출물은 앞으로 식물 기원의 살선충 물질 연구시 참고 자료로 활용될 수 있을 것이다.

Keywords

References

  1. Chatterjee, A., Sukul, N. C., Laskar, S. and Ghoshmajumdar, S. 1982. Nematicidal principles from two species of Lamiaceae. J. Nematol. 14: 118-120.
  2. Choi, I. H., Park, J. Y., Shin, S. C. and Park, I. K. 2006. Nematicidal activity of medicinal plant extracts and two cinnamates isolated from Kaempferia galanga L. (Proh Hom) against the pine wood nematode, Bursaphelenchus xylophilus. Nematol. 8: 359-365. https://doi.org/10.1163/156854106778493402
  3. Elbadri, G. A., Lee, D. W., Park, J. C., Yu, H. B. and Choo, H. Y. 2008. Evaluation of various plant extracts for their nematicidal efficacies against juveniles of Meloidogyne incognita. J. Asia-Pacific Entomol. 11: 99-102. https://doi.org/10.1016/j.aspen.2008.04.004
  4. Ferris, H. and Zheng, L. 1999. Plant sources of Chinese herbal remedies. J. Nematol. 31: 241-263.
  5. Germani, G., Reversat, G. and Luc, M. 1983. Effect of Sesbania rostrata on Hirschmanniella oryzae in flooded rice. J. Nematol. 15: 269-271.
  6. Grewal, P. S. 1989. Nematicidal effects of some plant extracts to Aphelenchoides composticola (Nematoda) infesting mushroom, Agaricus bisporus. Revue de Nematol. 12: 317-322.
  7. Heo, J. 1610. Dongeuibogam. CD-ROM. Yeogang Press, 1994. Seoul, Korea.
  8. Huang, K. C. 1993. The Pharmacology of Chinese Herbs. CRC Press, Ann Arbor, USA. 388 p.
  9. Johnson, A. W., Golden, A. M., Auld, D. L. and Sumner, D. R. 1992. Effects of rapeseed and vetch as green manure crops and fallow on nematodes and soil-borne pathogens. J. Nematol. 24: 117-126.
  10. Kim, D. G., Lee, Y. K., Lee, J. K. and Choi, Y. C. 1997. Simple cultivation of a Rhabditis sp. in nutrient broth. RDA. J. Crop Protec. 39: 42-46.
  11. Kim, H. J., Choo, H. Y., Park, C. G., Lee, S. M. and Kim, J. B. 1998. Biological control of northern root-knot nematodes, Meloidogyne hapla with plant extract. Korean J. Appl. Entomol. 37: 199-206.
  12. Kuhajek, J. M., Jeffers, S. N., Slattery, M. and Wedge, D. E. 2003. A rapid microbioassay for discovery of novel fungicides for Phytophthora spp. Phytopathology 93: 46-53. https://doi.org/10.1094/PHYTO.2003.93.1.46
  13. Lim, S. H., Zhu, Y. Z. Kim, M. S., Lee, Y. S., Son, J. S., Park, D. S., Hur, J. H., Kim, H. Y. and Choi, H, J. 2004. Nematicidal activity of Korean native plants against root-knot nematode, Meloidogyne incognita. Korean J. Pesticide Sci. 8: 353-357.
  14. MacGuidwin, A. E. and Layne, T. L. 1995. Response of nematode communities to sudangrass and sorghum-sudangrass hybrids grown as green manure crops. J. Nematol. 27: 609-616.
  15. McGaw, L. J., Van der Merwe, D. and Eloff, J. N. 2007. In vitro anthelmintic, antibacterial and cytotoxic effects of extracts from plants used in South African ethnoveterinary medicine. The Veterinary J. 173 : 366–372. https://doi.org/10.1016/j.tvjl.2005.09.004
  16. Miao, S. D. 1993. Zhon ghua yao hai. (The expanse of Chinese herbal medicines). Haerbin Publisher, Haerbin, China.
  17. McGaw, L. J., Van der Merwe, D. and Eloff, J. N. 2007. In vitro anthelmintic, antibacterial and cytotoxic effects of extracts from plants used in South African ethnoveterinary medicine. The Veterinary J. 173: 366–372. https://doi.org/10.1016/j.tvjl.2005.09.004
  18. Mojtahedi, H., Santo, G. S. and Ingham, R. E. 1993. Suppression of Meloidogyne chitwoodi with sudangrass cultivars as greenmanure. J. Nematol. 25: 303-311.
  19. Moriarty, Y. 1964. The efficacy of chrysoidin, new blue R and phloxine B for determining the viability of beet eelwonn, Heterodera schachtii Schm. Nematologica 10: 644-646. https://doi.org/10.1163/187529264X00295
  20. Oka, Y., Ben-Daniel, B. and Cohen, Y. 2012. Nematicidal activity of the leaf powder and extracts of Myrtus communis against the root-knot nematode Meloidogyne javanica. Plant Pathol. 61: 1012–1020. https://doi.org/10.1111/j.1365-3059.2011.02587.x
  21. Oostenbrink, M. 1960. Population dynamics in relation to croppings, manuring and soil disinfection. In: Nematology, ed. by J. N. Sasser and W. R. Jenkins, Univ. of North Carolina, USA.
  22. Pavaraj, M., Bakavathiappan, G. and Baskaran, S. 2012. Evaluation of some plant extracts for their nematicidal properties against root-knot nematode, Meloidogyne incognita. J. Biopest. 5: 106-110.
  23. Perry, L. M. 1980. Medicinal Plants of East and Southeast Asia. Massachusetts Institute of Technology, Cambridge, USA. 632 pp.
  24. Preiser, F. A. 1962. Study and development of comparative laboratory testing of nematicides. PhD. thesis. McGill University, Montreal, Canada.
  25. Prot., J. C., Soriano, I. R. S., Mantias, D. M. and Savary, S. 1992. Use of green manure crops in control of Hirschmanniella mucronata and H. oryzae in irrigated rice. J. Nematol. 24: 127-132.
  26. Satoshi T., Sawada, J. and Moromizato, Z. 2008. Nematicidal activity of Okinawa Island plants on the root-knot nematode Meloidogyne incognita (Kofoid and White) Chitwood. Plant Soil 303: 207-216. https://doi.org/10.1007/s11104-007-9499-7
  27. Siddiqui, M. A. and Alam, M. M. 1990. Toxicity of different plant parts of Tagetes lucida to plant-parasitic nematodes. Indian J. Nematol. 18: 181-185.
  28. Southey, J. F. 1986. Laboratory Methods for Work with Plant and Soil Nematodes. Her Majesty's Stationery Office, London, UK. 202 pp.
  29. Tarjan, A. C. 1955. Evaluation of various nematodes for use in contact nematicide tests. Proc. Helm. Soc. Wash. 22: 33-37.
  30. Taylor A. L. and Sasser, J. N. 1978. Biology, Identification and Control of Root-knot Nematodes (Meloidogyne Species). North Carolina State Univ., Graphics, USA. 111 pp.
  31. Verma, B. S., Verma, K. K., Sangwan, N. K. and Dhindsa, K. S. 1989. Toxicity of some indigenous plant extracts to root-knot, seedgall, and citrus nematodes. Pesticides(Bombay) 23: 25-57.
  32. Whapham, C. A., Jenkins, T., Blunden, G. and Hankins, S. D. 1994. The role of seaweed extracts, Acophyllum nodosum, in the reduction in fecundity of Meloidogyne javanica. Fund. Appl. Nematol. 17: 181-183.
  33. Wiratno D. T., Van den Bergb, H., Riksend, J. A. G., Rietjensb, I. M. C. M., Djiwantia, S. R., Kammengad, J. E. and Murkb, A. J. 2009. Nematicidal activity of plant extracts against the root-knot nematode, Meloidogyne incognita. The Open Nat. Prod. J. 2: 77-85. https://doi.org/10.2174/1874848100902010077
  34. Zhang, G. and Xia, G. 1996. Color Pictorial Handbook of Toxic Chinese Herbs. Xinhao Bookstore, Tiangzing, China.

Cited by

  1. Control Effect of Coffee Ground Compost and Velvet bean Against Root-Knot Nematode, Meloidogyne incognita in Pumpkin vol.20, pp.1, 2016, https://doi.org/10.7585/kjps.2016.20.1.47