The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Infection of Mycovirus in Imported Lentinula edodes

해외도입 표고버섯의 진균바이러스 감염

  • Lee, Song Hee (Department of Microbiology and Research Institute for Life Science, Gyeongsang National University) ;
  • Kwak, Seo-Young (Department of Microbiology and Research Institute for Life Science, Gyeongsang National University) ;
  • Ko, Han Kyu (Forest Mushroom Research Institute) ;
  • Lee, Hyun-Sook (Department of Microbiology and Research Institute for Life Science, Gyeongsang National University)
  • 이송희 (경상대학교 미생물학과) ;
  • 곽서영 (경상대학교 미생물학과) ;
  • 고한규 (산림조합중앙회 산림버섯연구소) ;
  • 이현숙 (경상대학교 미생물학과)
  • Received : 2014.03.06
  • Accepted : 2014.03.21
  • Published : 2014.03.31
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Abstract

Up to date several mycoviruses including Lentinula edodes Spherical Virus (LeSV) have been reported. As fungal virus was spreaded by infested hypae and spores it could be important to use virus-free spawns to eradicate the mushroom virus disease in the culture farm. We tested the imported spawns of Lentinula edodes by PCR whether LeSV was infested them or not. The primer set targeting the RdRp gene of LeSV was prepared based on partial sequence of the LeSV genome. The RT-PCR analysis showed that 87 among 88 imported spawns of L. edodes were infested by LeSV.

최근 몇몇 버섯의 바이러스가 보고되었으며, Lentinula edodes Spherical Virus (LeSV) 역시 보고되었다. 곰팡이 바이러스는 종균에 의해 전파되기 때문에 농장에서 버섯 바이러스병을 근절하기 위해서는 건전 종균을 사용하는 것이 중요하다. 이에 표고버섯 바이러스인 LeSV의 RdRp 유전자에 특이적인 primer 및 RT-PCR 조건을 이용하여 해외에서 도입한 표고종균을 대상으로 LeSV 감염도를 조사하였다. 분석결과 해외도입 종균의 99%가 LeSV에 감염된 것으로 나타났다.

Keywords

References

  1. MAFRA. Present situation and issues of mushroom industry;2005.
  2. Ridley SP, Sommer SS, Wickner RB. Superkiller mutations in Saccharomyces cerevisiae suppress exclusion of M2 doublestranded RNA by L-A-HN and confer cold sensitivity in the presence of M and L-A-HN. Mol Cell Biol 1984;4:761-770. https://doi.org/10.1128/MCB.4.4.761
  3. Hollings M. Viruses associated with dieback disease of cultivated mushroom. Nature 1962;196:962-965. https://doi.org/10.1038/196962a0
  4. Goodin MM, Schlagnhaufer B, Romaine CP. Encapsida- tion of the La France disease-specific double-stranded RNAs in 36 nm isometirc virus-like particles. Phytopathol 1992;82:285-290. https://doi.org/10.1094/Phyto-82-285
  5. Bruenn FA. A closely related group of RNA-dependent RNA polymerases from double-strand RNA viruses. Nucleic Acids Res 1993;21:5667-5669. https://doi.org/10.1093/nar/21.24.5667
  6. van der Lende TR, Harmsen MC, Wessels JGH. Doublestranded RNA and proteins associated with 34 nm virus particles of the cultivated mushroom Agaricus bisporus. J Gen Virol 1994;75:2533-2536. https://doi.org/10.1099/0022-1317-75-9-2533
  7. Schisler LC, Sinden JW, Sigel EM. Ethiology, symptomatology, and epidemiology of a virus disease of cultivated mushrooms. Phytopathology 1967;57:519-526.
  8. Marino R, Saksena KN, Schuler M, Mayfield JE, Lemke PA. Double-stranded ribonucleic acid in Agaricus bisporus. Appl Environ Microbiol 1976;31:433-438.
  9. Morris TJ, Dodds JA. Isolation and analysis of doublestranded RNA from virus-infected plant and fungal tissue. Phytopathology 1979;69:854-858. https://doi.org/10.1094/Phyto-69-854
  10. Harmsen MC, Tolner B, Karm A, Go SJ, de Haan A, Wessels JGH. Sequence of three dsRNAs associated with La France disease of the cultivated mushroom (Agaricus bisporus). Cuur Genet 1991;20:137-144. https://doi.org/10.1007/BF00312776
  11. van der Vlugt RAA, Schapp PJ, Muller Y, Thurstio CF, Sonnenberg ASM, Visser J, van Griensven LJLD. A codon usage table for Agaricus bisporus. CMR Newsletter 1993;1:50-52.
  12. Harmsen MC, van Griensven LJLD, Wessels JGH. Molecular analysis of Agaricus bisporus double stranded RNA. J Gen Virol 1989;79:1613-1616.
  13. Yu HJ, Lim DB, Lee HS. Characterization of a novel single stranded RNA mycovirus in Pleurotus ostreatus. Virology 2003;15:9-15.
  14. Ro HS, Lee NJ, Lee CW, Lee HS. Isolation of novel mycovirus OMIV in Pleurotus ostreatus and its cetection using a triple antibody sandwich-ELISA. J Virol Methods 2006;138: 24-29. https://doi.org/10.1016/j.jviromet.2006.07.016
  15. Kwon YC, Jeong DW, Gim SI, Ro HS, Lee HS. Curing viruses in Pleurotus ostreatus by growth on a limited nutrient medium containing cAMP and rifamycin. J Virol Methods 2012;185: 156-159. https://doi.org/10.1016/j.jviromet.2012.06.002
  16. Ro HS, Kang EJ, Yu JS, Lee TS, Lee CW, Lee HS. Isolation and characterization of novel mycovirus, PeSV, in Pleurotus eryngii and the development of a diagnostic system for it. Biotechnol Lett 2007;29:129-135.
  17. Ohata C, Taguchi T, Takahashi S, Ohtsuka K, Ayusawa S, Magae Y. Detection of double stranded RNA elements in cultivated Lentinula edodes. Mushroom Sci Biotechnol 2008;16: 155-158.
  18. Magae Y. Molecular characterization of a novel mycovirus in the cultivated mushroom Lentinula edodes. Virol J 2012;9:60-65. https://doi.org/10.1186/1743-422X-9-60
  19. Won HS, Park SJ, Kim DK, Shin MJ, Kim N, Lee SH, Kwon YC, Ko HK, Ro HS, Lee HS. Isolation and characterization of a mycovirus in Lentinula edodes. J Microbiol 2013;51:118-122. https://doi.org/10.1007/s12275-013-2351-2
  20. Korea Forest Service. Statistical yearbook of forestry; 2011.
  21. Kim JM, Yun SH, Song HY, Choi HJ, Ko YH, Oh JM, Kim DH. Biological function of a mycovirus LeV-FMR10339 in the edible mushroom Lentinula edodes FMR10339 strain. 2013 International Meeting of the federation of Korean Microbiologica Societies; October 17-18, Seoul, Korea, 2013. p. 135.