Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
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Genotoxicity (DNA damage) on Blood Cells of Parrot Fish (Oplegnathus fasciatus) Exposed to Acidified Seawater Making of CO2

이산화탄소로 산성화된 해수에 노출된 돌돔(Oplegnathus fasciatus) 혈구세포에 대한 유전독성(DNA 손상)

  • Choi, Tae Seob (Institute of Environmental Protection and Safety, NeoEnBiz Co.) ;
  • Lee, Ji-Hye (Institute of Environmental Protection and Safety, NeoEnBiz Co.) ;
  • Sung, Chan-Gyoung (Institute of Environmental Protection and Safety, NeoEnBiz Co.) ;
  • Lee, Jung-Suk (Institute of Environmental Protection and Safety, NeoEnBiz Co.) ;
  • Park, Young-Gyu (Ocean Circulation and Climate Research Division, Korea Institute of Ocean Science and Technology (KIOST)) ;
  • Kang, Seong-Gil (Offshore CCS research Unit, Korea Research Institute of Ships & Ocean Engineering (KRISO))
  • 최태섭 ((주)네오엔비즈 환경안전연구소) ;
  • 이지혜 ((주)네오엔비즈 환경안전연구소) ;
  • 성찬경 ((주)네오엔비즈 환경안전연구소) ;
  • 이정석 ((주)네오엔비즈 환경안전연구소) ;
  • 박영규 (한국해양과학기술원 해양순환.기후연구부) ;
  • 강성길 (한국해양과학기술원 해양시스템안전연구소)
  • Received : 2014.01.24
  • Accepted : 2014.03.12
  • Published : 2014.03.31
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Abstract

DNA damage such as genotoxicity was identified with comet assay, which blood cell of a marine parrot fish (Oplegnathus fasciatus) was exposed to an acidified seawater, lowered pH gradient making of $CO_2$ gas. The gradient of pH were 8.22, 8.03, 7.81, 7.55 with control as HBSS solution with pH 7.4. DNA tail moment of fish blood cell was $0.548{\pm}0.071$ exposed seawater of pH 8.22 condition, on the other hand, DNA tail moment $1.601{\pm}0.197$ exposed acidified seawater of pH 7.55 lowest condition. The approximate difference with level of DNA damage was 2.9 times between highest and lowest of pH. DNA damage with decreasing pH was significantly increased with DNA tail moment on blood cell of marine fish (ANOVA, p < 0.001). Ocean acidification, especially inducing the leakage of sequestered $CO_2$ in geological structure is a consequence from the burning of fossil fuels, and long term effects on marine habitats and organisms are not fully investigated. The physiological effects on adult fish species are even less known. This result shown that the potential of dissolved $CO_2$ in seawater was revealed to induce the toxic effect on genotoxicity such as DNA breakage.

Keywords

References

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