한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제43권3호
  • /
  • Pages.389-396
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  • 2014
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  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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추출방법에 따른 쇠비름의 항산화, 생리활성 및 Acetylcholinesterase 저해활성

Antioxidant, Physiological Activities, and Acetylcholinesterase Inhibitory Activity of Portulaca oleracea Extracts with Different Extraction Methods

  • 권유리 (대구가톨릭대학교 식품공학전공) ;
  • 조성묵 (대구가톨릭대학교 식품공학전공) ;
  • 황승필 (대구가톨릭대학교 식품공학전공) ;
  • 권기만 (대구가톨릭대학교 식품공학전공) ;
  • 김재원 (대구가톨릭대학교 식품공학전공) ;
  • 윤광섭 (대구가톨릭대학교 식품공학전공)
  • Kwon, Yu-Ri (Dept. of Food Science and Technology, Catholic University of Daegu) ;
  • Cho, Sung-Mook (Dept. of Food Science and Technology, Catholic University of Daegu) ;
  • Hwang, Seung-Pil (Dept. of Food Science and Technology, Catholic University of Daegu) ;
  • Kwon, Gi-Man (Dept. of Food Science and Technology, Catholic University of Daegu) ;
  • Kim, Jae-Won (Dept. of Food Science and Technology, Catholic University of Daegu) ;
  • Youn, Kwang-Sup (Dept. of Food Science and Technology, Catholic University of Daegu)
  • 투고 : 2013.11.08
  • 심사 : 2013.12.04
  • 발행 : 2014.03.31
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초록

쇠비름의 활용 및 생리활성을 증가시킬 수 있는 적정 추출방법을 알아보고자 환류냉각, 가압가열 및 저온고압 추출법을 이용하여 추출한 쇠비름 70% 에탄올 추출물의 생리활성을 비교하였다. 추출수율은 환류냉각추출, 가압가열추출, 저온고압추출 순으로 높은 수율을 나타내었다. 색도에서는 저온고압추출에서 명도와 황색도가 높았고, 채도의 경우 가압가열 및 저온고압추출에서 높았다. 폴리페놀 및 프로안토시아니딘 함량은 저온고압추출이 환류냉각 및 가압가열추출에 비해 높은 함량을 나타내었으며, 플라보노이드 함량은 가압가열추출에서 높은 함량을 나타내었다. 항산화 활성은 저온고압추출이 환류냉각 및 가압가열 추출에 비해 유의적으로 높은 활성을 나타낸 반면 철 이온에 대한 제거효과의 경우 환류냉각추출 및 가압가열추출에서 높은 활성을 나타내었다. ${\alpha}$-Glucosidase 및 XO 저해활성은 저온고압추출에서 가장 높은 활성을 나타내었다. 한편 AChE 저해활성에서는 저온고압추출, 가압가열추출, 환류냉각추출 순으로 높은 활성을 나타내었고, TLC bioassay를 통하여 살펴본 결과 특정 compound들에 의해 AChE 저해 활성이 나타났으며 퇴행성 질환의 하나인 치매의 예방 가능성을 확인하였다. 이러한 결과를 종합해 볼 때 저온고압추출물이 소재 활용가치가 높을 것으로 사료되며 천연항산화제 및 기능성 증진을 위한 소재로 이용 가능할 것으로 판단된다.

The physiological properties of 70% ethanol extracts from Portulaca oleracea with different extraction methods (reflux extraction, RE; autoclave extraction, AE; low temperature high pressure extraction, LTPE) were investigated. The freeze-dried powder yields of RE, AE, and LTPE were 33.78%, 30.80%, and 11.05%, respectively. The color values of L and b were higher in LTPE, and the chroma values were higher in AE and LTPE compared to RE. The total polyphenolics and proanthocyanidin contents in LTPE were significantly higher than in other extracts. The amount of substances related to flavonoids contents was highest in RE (4.30 mg/g), followed by AE (4.06 mg/g), and LTPE (4.00 mg/g). DPPH radical scavenging ability with a concentration of 500 mg% (w/v) were in the following order; LTPE (88.87%)> RE (83.84%)> AE (80.67%). Further, the reducing power, ABTS radical scavenging ability, and nitrite scavenging activity was observed in the same tendency as seen with the DPPH radical scavenging ability. However, the ferrous ion chelating activity of RE (85.45%) and AE (83.88%) was significantly higher than that of LTPE (75.60%). ${\alpha}$-Glucosidase inhibitory activities of RE and LTPE with a concentration of 100 mg% were significantly higher than AE. Xanthine oxidase, and acetylcholinesterase inhibitory activities of LTPE were higher than the other extracts. These results suggest that the extracts from Portulaca oleracea have the potential to act as functional materials, and components of Portulaca oleracea could be effective in the prevention of Alzheimer's disease, and may be used to develop various functional food products.

키워드

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