The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Bcl-2 Knockdown Accelerates T Cell Receptor-Triggered Activation-Induced Cell Death in Jurkat T Cells

  • Lee, Yun-Jung (Laboratory of Host Defense Modulation, College of Pharmacy, Chung-Ang University) ;
  • Won, Tae Joon (Laboratory of Host Defense Modulation, College of Pharmacy, Chung-Ang University) ;
  • Hyung, Kyeong Eun (Laboratory of Host Defense Modulation, College of Pharmacy, Chung-Ang University) ;
  • Lee, Mi Ji (Laboratory of Host Defense Modulation, College of Pharmacy, Chung-Ang University) ;
  • Moon, Young-Hye (Laboratory of Host Defense Modulation, College of Pharmacy, Chung-Ang University) ;
  • Lee, Ik Hee (Laboratory of Host Defense Modulation, College of Pharmacy, Chung-Ang University) ;
  • Go, Byung Sung (Laboratory of Host Defense Modulation, College of Pharmacy, Chung-Ang University) ;
  • Hwang, Kwang Woo (Laboratory of Host Defense Modulation, College of Pharmacy, Chung-Ang University)
  • Received : 2013.11.18
  • Accepted : 2014.01.16
  • Published : 2014.02.28
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Abstract

Cell death and survival are tightly controlled through the highly coordinated activation/inhibition of diverse signal transduction pathways to insure normal development and physiology. Imbalance between cell death and survival often leads to autoimmune diseases and cancer. Death receptors sense extracellular signals to induce caspase-mediated apoptosis. Acting upstream of CED-3 family proteases, such as caspase-3, Bcl-2 prevents apoptosis. Using short hairpin RNAs (shRNAs), we suppressed Bcl-2 expression in Jurkat T cells, and this increased TCR-triggered AICD and enhanced TNFR gene expression. Also, knockdown of Bcl-2 in Jurkat T cells suppressed the gene expression of FLIP, TNF receptor-associated factors 3 (TRAF3) and TRAF4. Furthermore, suppressed Bcl-2 expression increased caspase-3 and diminished nuclear factor kappa B (NF-${\kappa}B$) translocation.

Keywords

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