Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Prevalence of antimicrobial resistance and integrons in extended-spectrum β-lactamases producing Escherichia coli isolated from Nakdong and Gumho river

낙동강과 금호강에서 분리된 광범위 베타 락탐 분해효소 생성 Escherichia coli 내 항균제 내성 및 integrons의 분포

  • Cho, Jae-Keun (Metropolitan Health & Environmental Research Institute) ;
  • Kim, Hwan-Deuk (Metropolitan Health & Environmental Research Institute) ;
  • Kwon, Soon-Hyo (Metropolitan Health & Environmental Research Institute) ;
  • Kim, Jin-Hyun (Gyeongbuk Veterinary Service Laboratory) ;
  • Jang, Sung-Il (Metropolitan Health & Environmental Research Institute) ;
  • Park, Choi-Kyu (College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Ki-Seuk (College of Veterinary Medicine, Kyungpook National University)
  • 조재근 (대구광역시보건환경연구원) ;
  • 김환득 (대구광역시보건환경연구원) ;
  • 권순효 (대구광역시보건환경연구원) ;
  • 김진현 (경상북도가축위생시험소) ;
  • 장성일 (대구광역시보건환경연구원) ;
  • 박최규 (경북대학교 수의과대학) ;
  • 김기석 (경북대학교 수의과대학)
  • Received : 2014.01.02
  • Accepted : 2014.03.04
  • Published : 2014.03.30
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Abstract

This study was conducted to investigate the antimicrobial resistance, presence of ${\beta}$-lactamase genes and integrons in 83 ESBL-producing Escherichia coli isolated from Nakdong river and Geumho river in Daegu. Among the ${\beta}$-lactam antimicrobials, all isolates were resistant to ampicillin, cephalothin, cefamandole and cefotaxime, followed by piperacillin (98.8%), ampicillin/sulbactam (86.7%), aztreonam (60.2%) and cefepime (59.0%), whereas resistance to piperacillin/tazobacram, ticarcillin/clavulanic acid and cefoxitin was less than 30%. Many of the ESBL-producing Escherichia coli were also resistant to non-${\beta}$-lactams antimicrobials such as nalidixic acid (83.1%), sulfonamides (72.3%), ciprofloxacin (62.7%) and gentamicin (38.6%). All isolates showed resistance to seven or more antimicrobial agents. The most frequently detected gene was $bla_{TEM+CTX-M}$ (49.4%), followed by $bla_{CTX-M}$ (27.7%), $bla_{TEM}$ (6.0%) and $bla_{OXA}$ (1.2%). But $bla_{SHV}$ was not found. Class 1 integrons were found in 61.4% (51 isolates) of isolates, however, class 2 and 3 integrons were not detected. The results showed water from Nakdong river and Geumho river is contaminated with ESBL-producing E. coli isolates. These results suggest the need for further investigation of antibiotic resistant bacteria to prevent public health impacts in the water environment.

Keywords

References

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