Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Bactericidal Efficacy of a Fumigation Disinfectant with Ortho-phenylphenol as an Active Ingredient Against Pseudomonas Aeruginosa and Enterococcus Hirae

Ortho-phenylphenol을 주성분을 하는 훈증소독제의 Pseudomonas aeruginosa와 Enterococcus hirae에 대한 살균효과

  • Cha, Chun-Nam (Engineering Research Institute and Department of Industrial Systems Engineering, Gyeongsang National University) ;
  • Park, Eun-Kee (Department of Medical Humanities and Social Medicine, College of Medicine, Kosin University) ;
  • Kim, Yongpal (Elkahmco Bio Co., Ltd.) ;
  • Yu, Eun-Ah (Tongyeong National Quarantine Station, Ministry of Health & Welfare) ;
  • Yoo, Chang-Yeol (Department of Computer Information, Gyeongnam Provincial Namhae College) ;
  • Hong, Il-Hwa (Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Suk (Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Hu-Jang (Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University)
  • 차춘남 (경상대학교 공과대학 공학연구원) ;
  • 박은기 (고신대학교 의과대학) ;
  • 김용팔 (엘캄코 바이오(주)) ;
  • 유은아 (보건복지부 통영검역소) ;
  • 유창열 (경남도립남해대학 인터넷정보학과) ;
  • 홍일화 (경상대학교 수의과대학 생명과학연구원) ;
  • 김석 (경상대학교 수의과대학 생명과학연구원) ;
  • 이후장 (경상대학교 수의과대학 생명과학연구원)
  • Received : 2013.11.13
  • Accepted : 2014.02.14
  • Published : 2014.03.30
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Abstract

This test was performed to evaluate the bactericidal efficacy of a fumigation disinfectant containing 20% ortho-phenylphenol against Pseudomonas aeruginosa (P. aeruginosa) and Enterococcus hirae (E. hirae). In preliminary tests, P. aeruginosa and E. hirae working culture suspension number (N value) were $2.8{\times}10^8$ and $4.0{\times}10^8CFU/mL$, respectively. And all the colony numbers on the carriers exposed to the fumigant (n1, n2, n3) were higher than 0.5N1 (the number of bacterial test suspentions by pour plate method), 0.5N2 (the number of bacterial test suspentions by filter membrane method) and 0.5N1, respectively. In addition, the mean number of P. aeruginosa and E. hirae recovered on the control-carriers (T value) was $2.8{\times}10^8$ and $3.4{\times}10^6CFU/mL$, respectively. In the bactericidal effect of the fumigant, the reduction number of $2.8{\times}10^8$ (d value) was 6.46 and 5.19 logCFU/mL, respectively. According to the French standard for the fumigant, the d value for the effective bactericidal fumigant should be over than 5 logCFU/mL. With the results from this study, the fumigation disinfectant containing 20% ortho-phenylphenol has an effective bactericidal activity, then the fumigant can be applied to disinfect food materials and kitchen appliances contaminated with the pathogenic bacteria.

본 연구는 P. aeruginosa와 E. hirae을 대상으로 ortho-phenylphenol 20%를 함유한 훈증소독제, Fumagari $OPP^{(R)}$의 살균효과를 평가하기 위해 수행되었다. 예비 시험에서, P. aeruginosa와 E. hirae의 현탁액 균수는 각각 $2.8{\times}10^8$와 $3.6{\times}10^8CFU/mL$이었으며, 모든 훈증소독제에 노출시킨 담체의 균수는 모두 평판배지법과 여과법으로 배양한 시험균주 현탁액의 균수의 50%보다 많았다. 또한, 대조 담체로부터 회복된 P. aeruginosa와 E. hirae 균수는 각각 $2.9{\times}10^6$와 $2.7{\times}10^6CFU/mL$이었다. 훈증소독제의 살균효과 시험에서는, 훈증소독제를 처리한 담체의 P. aeruginosa와 E. hirae의 감소 균수는 각각 6.46와 5.19 logCFU/mL로 나타났다. 이상의 결과로부터, 훈증소독제, Fumagari $OPP^{(R)}$는 P. aeruginosa와 E. hirae에 대해 효과적인 살균력을 갖는 것으로 확인되었으며, 병원성 세균에 오염된 식품재료 및 주방용품의 소독에 적용할 수 있을 것으로 사료된다.

Keywords

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