Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Isolation and Identification of DHA-Rich Marine Microorganism

Docosahexaenoic acid (DHA)를 다량 함유하는 해양미생물의 분리 및 동정

  • Jeong, U-Cheol (Department of Marine Biology and Aquaculture/Institute of Marine Industry, Gyeongsang National University) ;
  • Choi, Byeong-Dae (Department of Seafood Science and Technology, Gyeongsang National University) ;
  • Choi, Jong-Duck (Department of Seafood Science and Technology, Gyeongsang National University) ;
  • Kang, Seok-Joong (Department of Marine Biology and Aquaculture/Institute of Marine Industry, Gyeongsang National University)
  • 정우철 (경상대학교 해양생명과학과/해양산업연구소) ;
  • 최병대 (경상대학교 해양식품공학과) ;
  • 최종덕 (경상대학교 해양식품공학과) ;
  • 강석중 (경상대학교 해양생명과학과/해양산업연구소)
  • Received : 2013.02.06
  • Accepted : 2013.10.08
  • Published : 2014.02.28
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Abstract

Docosahexaenoic acid (DHA, 22:6n-3) and ecosapentaenoic acid (20:5n-3) have attracted increasing attention since the first epidemiological report on the importance of n-3 essential fatty acids. It is thought that DHA has important functions in brain and retinal tissues. Thraustochytrids, a group of marine protists, are capable of heterotrophic growth, and are potential omega-3 producers for industrial use, especially the members of the Schizochytrium and Thraustochytrium genera. The aims of this work were to isolate, identify and screen thraustochytrids from 17 different locations. Twenty-three isolates were screened for biomass, total fatty acid (TFA) and DHA content. Analysis of the fatty acid methyl esters revealed four distinct clusters biomass ranged from $8.68-9.36gL^{-1}$, and lipid and DHA contents ranged from $3.11-4.10gL^{-1}$ and $1.05-1.93gL^{-1}$ biomass, respectively. B-12 isolates were screened for biomass ($9.36gL^{-1}$), TFA ($4.10gL^{-1}$) and DHA (47.01%, w/w) content. C-6 isolates were also screened for biomass ($8.92gL^{-1}$), TFA ($3.30gL^{-1}$) and DHA (49.41%, w/w) content. The 18S rRNA gene sequencing results identified Schizochytrium mangrovei as B-12 and Crypthecodium cohnii as C-6.

Keywords

References

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