Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
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Expression of Nuclear Factor Erythroid 2 Protein in Malignant Cutaneous Tumors

  • Choi, Chang Yong (Department of Plastic and Reconstructive Surgery, Soonchunhyang Gumi Hospital, Soonchunhyang University College of Medicine) ;
  • Kim, Jin Young (Department of Plastic and Reconstructive Surgery, Soonchunhyang Gumi Hospital, Soonchunhyang University College of Medicine) ;
  • Wee, Seo Yeong (Department of Plastic and Reconstructive Surgery, Soonchunhyang Gumi Hospital, Soonchunhyang University College of Medicine) ;
  • Lee, Jang Hyun (Department of Plastic and Reconstructive Surgery, Hanyang University Guri Hospital, Hanyang University College of Medicine) ;
  • Nam, Doo Hyun (Department of Plastic and Reconstructive Surgery, Soonchunhyang University Cheonan Hospital) ;
  • Kim, Chul Han (Department of Plastic and Reconstructive Surgery, Soonchunhyang University College of Medicine) ;
  • Cho, Moon Kyun (Molecular Cancer Research, Soonchunhyang University College of Medicine) ;
  • Lee, Yoon Jin (Molecular Cancer Research, Soonchunhyang University College of Medicine) ;
  • Nam, Hae Seon (Molecular Cancer Research, Soonchunhyang University College of Medicine) ;
  • Lee, Sang Han (Molecular Cancer Research, Soonchunhyang University College of Medicine) ;
  • Ch, Sung Woo (Molecular Cancer Research, Soonchunhyang University College of Medicine)
  • Received : 2014.01.03
  • Accepted : 2014.02.12
  • Published : 2014.11.15
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Abstract

Background Reactive oxygen species (ROS) damages cell molecules, and modifies cell signaling. The nuclear factor E2-related factor (Nrf2) is a critical transcription regulator, which protects cells against oxidative damage. Nrf2 expression is increased in a large number of cancers. However, little information has been reported regarding the expression of Nrf2 in skin cancers. Hence, we explored the expression of Nrf2 protein in skin cancers. Methods The Nrf2 protein expression in 24 specimens, including 6 malignant melanomas (MM), 6 squamous cell carcinomas (SCC), 6 basal cell carcinomas (BCC), and 6 normal skin tissues, was evaluated by western blotting. Immunohistochemical staining was performed. The expression of Kelch-like ECH-associated protein 1 (Keap1), the key regulator of Nrf2, was also analyzed by western blotting. Results Small interfering RNA transfection to the melanoma cell line G361 confirmed that an approximately 66 kDa band was the true Nrf2 band. The western blot revealed that the Nrf2 protein was definitely expressed in normal skin tissues, but the Nrf2 expression was decreased in MM, SCC, and BCC. Immunohistochemical examination showed that expression of Nrf2 was decreased in all skin cancer tissues compared to the normal skin tissues. Keap1 was not expressed in all malignant skin tumors and normal skin tissues by western blot. Conclusions ROS was increased in various types of cancers which proteins were highly expressed or underexpressed. This study demonstrated that the expression of Nrf2 protein was down-regulated in human malignant skin tumors. We suggest that decreased expression of Nrf2 is related to skin cancers.

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References

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