Journal of Marine Bioscience and Biotechnology (한국해양바이오학회지)

The Korean Society for Marine Biotechnology (한국해양바이오학회)
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Investigation of Microalgal Growth, Tetraselmis sp. KCTC12432BP by Supplying Bicarbonate on the Ocean Cultivation

해양배양기 내 중탄산염 공급에 따른 Tetraselmis sp. KCTC12432BP 증식에 관한 연구

  • Cho, Yonghee (Department of Biological Engineering, Inha University) ;
  • Shin, Dong-Woo (Department of Biological Engineering, Inha University) ;
  • Lee, Sangmin (Department of Biological Engineering, Inha University) ;
  • Jeon, Hyonam (Department of Biological Engineering, Inha University) ;
  • Ryu, Young-Jin (Department of Biological Engineering, Inha University) ;
  • Lee, Jong-Chan (Department of Biological Engineering, Inha University) ;
  • Lim, Sang-Min (Department of Biological Engineering, Inha University) ;
  • Lee, Choul-Gyun (Department of Biological Engineering, Inha University)
  • Received : 2014.12.30
  • Accepted : 2015.01.14
  • Published : 2014.12.31
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Abstract

The ocean provide great benefits for microalgal mass cultures with maintaining stable temperature due to high specific heat, mixing by wave energy, and providing large area for large-scale microalgae cultures. In this study, we cultivated a marine green microalga, Tetraselmis sp. KCTC12432BP, using marine photobioreactors on the ocean for investigating the effect of $NaHCO_3$ concentration on the biomass productivities and evaluating the potential of ocean microalgae culture. The culture medium consist of three fold concentrated f/2-Si with 4 g/L of $NaHCO_3$, which is dissolved in natural seawater. After 11 days of cultivation, the cultures reached stationary phase at biomass concentration of 1.6 g/L. At that time, $NaHCO_3$ concentration of 0, 2, and 4 g/L were fed to the cultures. The daily productivities of 0.11, 0.19, 0.30 g/L/day were attained with feeding rate of 0, 2, and 4 g/L $NaHCO_3$, respectively. Biomass productivity of Tetraselmis sp. KCTC12432BP was a function of the $NaHCO_3$ feeding rate as expected. This research shows that the microalgae can grow with $NaHCO_3$ as carbon source in marine photobioreactors on the ocean while exploiting various benefits of ocean cultivation.

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