Journal of Marine Bioscience and Biotechnology (한국해양바이오학회지)

The Korean Society for Marine Biotechnology (한국해양바이오학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Antimicrobial Activity of the Glutaraldehyde Cross-linked Glucosamine

글루코사민-글루타르알데히드 가교결합체의 항균 효과

  • Lee, Choon Geun (Department of Biomedical Science, College of Bio-nano, Gachon University) ;
  • Hwang, You Jin (Department of Life Science, College of Bio-nano, Gachon University) ;
  • Park, Jae Kweon (Department of Life Science, College of Bio-nano, Gachon University)
  • 이춘근 (가천대학교 바이오나노학과 의생명과학전공) ;
  • 황유진 (가천대학교 바이오나노대학 생명과학과) ;
  • 박제권 (가천대학교 바이오나노대학 생명과학과)
  • Received : 2014.12.05
  • Accepted : 2014.12.15
  • Published : 2014.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was investigated the antimicrobial activity of glutaraldehyde cross-linked glucosamine. Glutaraldehyde was used as a cross-linker which specifically combines an amine-group of molecules. To optimize the mixing ratio of glutaraldehyde and glucosamine, mixing ratio was set up 1:1, 2:1, 3:1 and 0.5:1 in molarity, respectively. The optimum mixing ratio of glucosamine and glutaraldehyde was found to be 3:1 using thin layer chromatography based on the production of complex. Glucosamine-glutaraldehyde cross-linked complex (Ggcc) revealed significant antimicrobial activity toward PWG than F1, both microbial strains were isolated from porcine semen as antibiotics resistance bacteria (ARB). These results clearly demonstrate that Ggcc has potential bactericidal activity toward ARB in porcine semen.

Keywords

References

  1. Anderson, J. W., Nicolosi, R. J. and Borzelleca, J. F. 2005. Glucosamine effects in humans: a review of effects on glucose metabolism, side effects, safety considerations and efficacy. Food Chem. Toxicol. 43, 187-201. https://doi.org/10.1016/j.fct.2004.11.006
  2. Bissett, D. L. 2006. Glucosamine: an ingredient with skin and other benefits. J. Cosmet. Dermatol. 5(4), 309-315. https://doi.org/10.1111/j.1473-2165.2006.00277.x
  3. Campos, E., Coimbra, P. and Gil, M. H. 2013. An improved method for preparing glutaraldehyde cross-linked chitosan-poly(vinyl alcohol) microparticles. Polym. Bull. 70, 549-561. https://doi.org/10.1007/s00289-012-0853-4
  4. Donzelli, B. G., Ostroff, G. and Harman, G. E. 2003. Enhanced enzymatic hydrolysis of langostino shell chitin with mixtures of enzymes from bacterial and fungal sources. Carbohydr. Res. 338, 1823-1833. https://doi.org/10.1016/S0008-6215(03)00269-6
  5. Elzoghby, A. O., Samy, W. M. and Elgindy, N. A. 2012. Albumin-based nanoparticles as potential controlled releas e drug delivery systems. J. Control. Release. 157, 168-182. https://doi.org/10.1016/j.jconrel.2011.07.031
  6. Gough, J. E., Scotchford, C. A. and Downes, S. 2002. Cytotoxicity of glutaraldehyde crosslinked collagen/poly (vinyl alcohol) films is by the mechanism of apoptosis. J. Biomed. Mater. Res. 61(1), 121-130.
  7. Jayakrishnan, A. and Jameela, S. R. 1996. Glutaraldehyde as a fixative in bioprostheses and drug delivery matrices. Biomaterials. 17, 471-484. https://doi.org/10.1016/0142-9612(96)82721-9
  8. Lee, Y. H. 2003. Study on multi-functional chitosan in cosmetics. Kor. J. Aesthet. Cosmetol. 1(3), 119-126.
  9. Rahman, M. H., Hjeljord, L. G., Aam, B. B., Sørlie, M. and Tronsmo A. 2014. Antifungal effect of chito-oligosac charides with different degrees of polymerization. Eur. J. Plant. Pathol. Published Online.
  10. Reginster, J. Y., Deroisy, R., Rovati, L. C., Lee, R. L., Lejeune, E., Bruyere, O., Glacovelli, G., Henrotin, Y., Dacre, J. E. and Gossett, C. 2001. Long-term effects of glucosamine sulphate on osteoarthritis progression: a randomised, placebo-controlled clinical trial. Lancet. 357, 251-256. https://doi.org/10.1016/S0140-6736(00)03610-2
  11. Richy, F. and Henrotin, Y. 2003. Structural and symptomatic efficacy of glucosamine and chondroitin in knee osteoarthritis: a comprehensive meta-analysis. Arch. Intern. Med. 163, 1514-1522. https://doi.org/10.1001/archinte.163.13.1514
  12. Sun, H. W., Feigal, R. J. and Messer H. H. 1990. Cytotoxicity of glutaraldehyde and formaldehyde in relation to time of exposure and concentration. Pediatr. Dent. 12(5), 303-307.
  13. Wang, T., Turhan, M. and Gunasekaran, S. 2004. Selected properties of pH-sensitive, biodegradable chitosan-poly(vinyl alcohol) hydrogel. Polym. Int. 53, 911-918. https://doi.org/10.1002/pi.1461