Journal of Sericultural and Entomological Science (한국잠사곤충학회지)

Korean Society of Sericultural Science (한국잠사학회)
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Antioxidant and antityrosinase activity of fermented silkworm hemolymph

발효 누에 숙잠 혈림프의 항산화 및 항티로시나제 활성

  • Mun, Ji-Young (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Jo, You-Young (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Kweon, HaeYong (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Lee, Kwang-Gill (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Yeo, Joo-Hong (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Lee, Heui-Sam (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
  • 문지영 (농촌진흥청 국립농업과학원 잠사양봉소재과) ;
  • 조유영 (농촌진흥청 국립농업과학원 잠사양봉소재과) ;
  • 권해용 (농촌진흥청 국립농업과학원 잠사양봉소재과) ;
  • 이광길 (농촌진흥청 국립농업과학원 잠사양봉소재과) ;
  • 여주홍 (농촌진흥청 국립농업과학원 잠사양봉소재과) ;
  • 이희삼 (농촌진흥청 국립농업과학원 잠사양봉소재과)
  • Received : 2014.10.03
  • Accepted : 2014.11.14
  • Published : 2014.10.31
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Abstract

In this study, functionality of products of silkworm hemolymph fermented by Bacillus species was studied such as cell viability, antioxidant effect, and inhibitory effect on tyrosinase activities. A matured silkworm hemolymph was degraded by fermentation with Bacillus subtilis 10854 and Bacillus amyloliquefaciens M27. Especially, proteins of matured silkworm hemolymph were degraded to 3,000 Da by fermentation with B. amyloliquefaciens M27. Cell viability for MTT assay was higher than PBS in hemolymph and fermented hemolymph. A DPPH free radical scavenging activity of fermented hemolymph was as higher as Vitamin C and dependent on sample concentrations. Thus, these results suggest that degraded hemolymph fermented by B. amyloliquefaciens M27 may have antioxidant properties as a material for cosmetics.

본 연구는 누에 숙잠 혈림프를 발효를 통하여 화장품으로서의 기능성과 피부 침투력을 증진시기고자 수행되었다. 누에 숙잠 혈림프를 대량 정제하여 이를 이용하여 2종의 Bacillus균을 이용하여 4주간 발효하였으며, 이들 발효산물의 분자량 및 세포 독성, 항산화, 티로시나아제 활성 억제 효과를 확인하였다. 1. 누에 숙잠 혈림프 단백질의 분자량이 커서(70 kDa) 정제물의 용해도가 낮다는 문제가 제기되어 단백질의 저분자화를 위해 바실러스속 균주 2종(B. subtilis 10854, B. amyloliquefaciens M27)으로 4주간 발효시켰다. 그 결과, 혈림프 발효액의 단백질은 현저하게 분자량이 저하되었으며, 바실러스속 균주 중 B. amyloliquefaciens M27이 단백질 분해 능력이 더 뛰어난 것으로 나타났다. 2. 누에 숙잠 혈림프 정제물을 바실러스속 균주 2종(B. subtilis 10854, B. amyloliquefaciens M27)으로 발효하여 발효액의 세포독성을 멜라닌세포의 세포 생존율을 측정하여 확인한 결과, 발효액의 $12.5{\sim}50{\mu}g/mL$ 농도 범위에서 세포 독성이 나타나지 않았고 세포 생존율이 증가하는 결과를 보였다. 3. 누에 숙잠 혈림프, 혈림프 발효물의 DPPH 자유라디칼 소거 활성을 정한 결과, 농도 의존적으로 항산화력이 증가하였고 혈림프 발효액은 양성대조군인 비타민 C와 유사한 활성 정도를 나타내었다. 분자량 저하 효과가 더 우수했던 Bacillus amyloliquefaciens M27균주를 이용하여 4주간 발효시키며 발효기간별 DPPH 자유라디칼 소거 활성을 측정한 결과, 2주 이상 발효시켰을 때 약 5% 이상 자유라디칼 소거 활성 능력이 증가함을 나타내었다. 4. 티로시나아제 활성 억제효과는 대조군에 비해 혈림프와 혈림프 발효액 처리군 모두에서 나타났으며, amyloliquefaciens M27를 이용한 발효액의 경우 미백효과가 더욱 뚜렷하였다.

Keywords

References

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