Journal of Sericultural and Entomological Science (한국잠사곤충학회지)

Korean Society of Sericultural Science (한국잠사학회)
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Antioxidant activities of black soldier fly, Hermetia illucens

아메리카동애등에(Hermetia illucens) 추출물의 항산화 활성

  • Park, Kwanho (National Academy of Agricultural Science, RDA) ;
  • Choi, Jiyoung (National Academy of Agricultural Science, RDA) ;
  • Nam, Sunghee (National Academy of Agricultural Science, RDA) ;
  • Kim, Sunghyun (National Academy of Agricultural Science, RDA) ;
  • Kwak, Kyuwon (National Academy of Agricultural Science, RDA) ;
  • Lee, Seokhyun (National Academy of Agricultural Science, RDA) ;
  • Nho, Sikab (College of Agriculture and Life science, Kyungpook National University)
  • 박관호 (농촌진흥청 국립농업과학원) ;
  • 최지영 (농촌진흥청 국립농업과학원) ;
  • 남성희 (농촌진흥청 국립농업과학원) ;
  • 김성현 (농촌진흥청 국립농업과학원) ;
  • 곽규원 (농촌진흥청 국립농업과학원) ;
  • 이석현 (농촌진흥청 국립농업과학원) ;
  • 노시갑 (경북대학교 농업생명과학대학)
  • Received : 2014.09.29
  • Accepted : 2014.11.05
  • Published : 2014.10.31
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Abstract

The purpose of this study was to determine the possibility of black soldier fly (Hermetia illucens) as a animal feedstuff. Insect resources have been widely recognized that it exhibits its own biological activity by whole body or its metabolic intermediates. To accomplish this, its general and biological activities were measured. The present study was conducted to compare antioxidant activity of black soldier fly larva, pupa and grasshopper by examining radical scavenging activity using DPPH (2,2 diphenyl 1-picryl hydrazyl). DPPH radical scavenging activity was conducted on the EtOH, MeOH, H2O fractions of black soldier fly larvae. Results showed that black soldier fly pupa on the H2O fraction contained the highest DPPH radical scavenging activity among the samples. FRAP assay are more suitable methods to evaluate antioxidant activity of black soldier fly extracts. Among the samples, the FRAP value showed higher antioxidant activity in the extracts from black soldier fly pupa extract on H2O fraction. These results suggest that black soldier fly larva and pupa extract has antioxidant activity and its fractions can be utilized to develop functional feedstuff.

최근 곤충자원을 이용하여 식약용 및 사료로 활용하기 위한 많은 연구가 진행되고 있으며 미래의 자원으로 발전할 수 있는 가능성이 충분하다고 본다. 특히, 대량으로 생산이 용이하고 유충과 번데기의 조단백질 및 무기물 함량이 우수한 아메리카동애등에는 양계 및 양어사료의 원료로써 사용 가능성이 높게 평가되고 있다. 본 연구는 아메리카동애등에의 유충 및 번데기의 항산화 활성을 비교 평가하였다. 아메리카동애등에 번데기의 물 추출물의 경우 DPPH 라디칼 소거능이 $147.8{\pm}1.6%$로 양성 대조군으로 사용된 Vitamin C(5 mg/mL)의 $109.0{\pm}0.15%$와 메뚜기의 $109.5{\pm}28%$ 보다 높은 활성을 나타내었다. FRAP을 통한 환원력 측정에서도 DPPH 결과와 마찬가지로 아메리카동애등에 번데기의 물 추출물에서 가장 높은 환원력을 나타내었다. 위의 결과로 보아, 아메리카동애등에 유충 및 번데기의 추출물은 라디칼 소거능 및 산화환원작용을 통하여 항산화 활성을 가지는 것으로 사료되며, 특히 번데기의 추출물은 천연 항산화 소재로서의 활용가능성이 높은 것으로 기대되었다. 추후 추출물의 산업적인 활용가능성을 보다 명확히 확인하기 위하여 보다 많은 연구가 필요할 것으로 본다.

Keywords

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