Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Trichomonas vaginalis Metalloproteinase Induces mTOR Cleavage of SiHa Cells

  • Quan, Juan-Hua (Department of Gastroenterology, The Affiliated Hospital of Guangdong Medical College) ;
  • Choi, In-Wook (Department of Infection Biology, Chungnam National University School of Medicine) ;
  • Yang, Jung-Bo (Department of Obstetrics and Gynecology, Chungnam National University Hospital) ;
  • Zhou, Wei (Department of Infection Biology, Chungnam National University School of Medicine) ;
  • Cha, Guang-Ho (Department of Infection Biology, Chungnam National University School of Medicine) ;
  • Zhou, Yu (Department of Gastroenterology, The Affiliated Hospital of Guangdong Medical College) ;
  • Ryu, Jae-Sook (Department of Environmental Biology and Medical Parasitology, Hanyang University College of Medicine) ;
  • Lee, Young-Ha (Department of Infection Biology, Chungnam National University School of Medicine)
  • Received : 2014.04.08
  • Accepted : 2014.09.29
  • Published : 2014.12.31
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Abstract

Trichomonas vaginalis secretes a number of proteases which are suspected to be the cause of pathogenesis; however, little is understood how they manipulate host cells. The mammalian target of rapamycin (mTOR) regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, and transcription. We detected various types of metalloproteinases including GP63 protein from T. vaginalis trophozoites, and T. vaginalis GP63 metalloproteinase was confirmed by sequencing and western blot. When SiHa cells were stimulated with live T. vaginalis, T. vaginalis excretory-secretory products (ESP) or T. vaginalis lysate, live T. vaginalis and T. vaginalis ESP induced the mTOR cleavage in both time-and parasite load-dependent manner, but T. vaginalis lysate did not. Pretreatment of T. vaginalis with a metalloproteinase inhibitor, 1,10-phenanthroline, completely disappeared the mTOR cleavage in SiHa cells. Collectively, T. vaginalis metallopeptidase induces host cell mTOR cleavage, which may be related to survival of the parasite.

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References

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