Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Effects of TRPV1 in formalin-induced nociceptive behavior in the orofacial area of rats

흰쥐의 구강악안면 영역에서 포르말린 통증행위반응에 TRPV1 채널의 영향

  • Received : 2013.11.25
  • Accepted : 2014.01.09
  • Published : 2014.01.31
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Abstract

The present study investigated the inflammatory hypersensitivity following activation of the transient receptor potential vanilloid receptor-1(TRPV1) in rats. Experiments were carried out on male Sprague-Dawley rats weighing 220-260g. Following an subcutaneous injection of 5% formalin in the orofacial area, nociceptive scratching behavior was recorded for 9 successive 5-min intervals in rats. The subcutaneous injection of $25{\mu}L$ of 5% formalin produced noxious scratching behavior. Injection of capsaicin, TRPV1 agonist, alone into the vibrissa pad did not produced nociceptive behavior. After subcutaneous injection of capsaicin(0$0.1{\mu}g$, $1{\mu}g/10{\mu}L$) in the formalin-treated rats, nociceptive scratching behavior was recorded for 9 successive 5-min internals. Injection of capsaicin into the vibrissa pad significantly increased the number of scratches at 1 hours after injection. Noxious behavioral responses induced by subcutaneous capsaicin injection were significantly potentiated in formalin-treated rats. Pre or post-treatment with iodo-resinaferatoxin(IRTX), TRPV1 antagonist, significantly attenuated increased nociceptive behavior. These findings suggest that activation of the TRPV1 enhanced formalin-induced inflammatory pain in the orofacial area of rats.

본 연구는 TRPV1 채널의 활성화가 염증성 통증반응 조절에 관여하는지에 관해 조사하였다. 수컷의 Sprague-Dawley계 흰 쥐 (220-260g)을 사용하였으며, 통증행위반응은 약물 주입 후 총 45분간 안면부를 긁거나 문지르는 행위를 지표로 간주하였다. 5% formalin($25{\mu}L$)을 단독투여 한 그룹에서는 유의한 통증행위반응을 관찰할 수 있었으나, capsaicin($0.1{\mu}g$, $1{\mu}g/10{\mu}L$)의 단독 투여한 그룹에서는 통증행위반응의 변화가 나타나지 않았다. 실험동물의 안면부에 TRPV1 채널의 효현제인 capsaicin 을 1시간 전에 주입한 후 동일 부위로 5% formalin 을 투여하게 되면 유의하게 증가된 통증행위반응을 관찰할 수 있었다. 증가된 통증행위반응은 TRPV1의 억제제인 I-RTX의 전처리에 의해 효과적으로 경감되었다(p<0.05). 이상의 결과들은 5% formalin으로 유발된 안면부 염증성 통증행위반응에 TRPV1의 활성화가 통증전도에 영향을 미칠 수 있음을 제시한다.

Keywords

References

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