DOI QR코드

DOI QR Code

곤충병원성 곰팡이 Metarhizium anisopliae FT83의 파밤나방 생육단계별 살충활성

Insecticidal Activity of Metarhizium anisopliae FT83 against the Different Stages of Beet Armyworm, Spodoptera exigua

  • 한지희 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 김정준 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 이상엽 (농촌진흥청 국립농업과학원 농업미생물과)
  • Han, Ji Hee (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Jeong Jun (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Lee, SangYeob (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA)
  • 투고 : 2014.11.12
  • 심사 : 2014.12.09
  • 발행 : 2014.12.31

초록

난방제 해충인 파밤나방의 친환경적 방제를 위하여 토양에서 곤충병원성 곰팡이를 수집한 다음, 파밤나방 유충에 대해 우수한 방제효과가 있는 곰팡이 Metarhizium anisopliae FT83을 분리하여 파밤나방의 생육단계별 살충율을 검정하였다. M. anisopliae FT83의 파밤나방 1령~3령 유충에 대한 살충율은 $1{\times}10^8conidia/ml$ 수준에서 100%이었고 반수치사시간은 0.5일, 2.6일, 2.5일이었으며 4령과 5령에 대한 살충율은 각각 $83.3{\pm}6.2%$, $86.0{\pm}5.7%$였으며 반수치사시간은 4.2일, 3.6일이었다. 번데기와 알에 대한 살충율도 100%로 파밤나방의 전 생육단계에 대해 높은 살충활성을 나타내었다.

The beet armyworm, Spodoptera exigua is pest which is difficult to control. For eco-friendly beet armyworm managements, we isolated entomopathogenic fungi from soil samples by insect-bait method using Tenebrio molitor and conducted bioassay to larvae of beet armyworm. The result of bioassay, a selected strain Metarhizium anisopliae FT83 caused 100% mortality against first ~ third instar larva of S. exigua at $1{\times}10^8conidia/ml$ and medial lethal time ($LT_{50}$) were 0.5 days, 2.6 days and 2.5 days respectively. Mortality against fourth and fifth larvae were $83.3{\pm}6.2%$ and $86.0{\pm}5.7%$ and medial lethal time ($LT_{50}$) were 4.2 days and 3.6 days respectively. Mortality against pupae and eggs of S. exigua were 100%. M. anisopliae FT83 showed high virulence at all developmental stages of S. exigua.

키워드

참고문헌

  1. Han, J. H., H. Y. Kim, H.T. Leem, J. J. Kim and S. Y. Lee (2013) Characteristics and virulence assay of entomopathogenic fungus Metarhizium anisopliae for the microbial control of Spodoptera exigua. Korean J. Pestic. Sci. 17: 454-459. https://doi.org/10.7585/kjps.2013.17.4.454
  2. Jin, N. Y., S. Y. Jung, C. Park, S. K. Paek, M. J. Seo and Y. Y. Man (2009) The synergy effects of mixed treatment with tannic acid and Bacillus thuringiensis subsp. kurstaki KB100 against Spodoptera exigua. Korean J. Appl. Entomol. 48(4):519-526. https://doi.org/10.5656/KSAE.2009.48.4.519
  3. Kang, E. J., M. G. Kang, M. J. Seo, S. N. Park, C. U. Kim and Y. M. Yu(2008) Toxicological effects of some insecticides against welsh onion beet armyworm (Spodoptera exigua). Korean J. Appl. Entomol. 47:155-162 https://doi.org/10.5656/KSAE.2008.47.2.155
  4. Marcos, R. F. and P. W. Stephen (2007) Mycoinsecticides and mycoacaricides : A comprehensive list with worldwide coverage and international classification of formulation types. Biol. Control. 43:237-256. https://doi.org/10.1016/j.biocontrol.2007.08.001
  5. Meyling, N. V. (2007) Methods for isolation of entomopathogenic fungi from the soil environment. Manual for isolation of soil borne entomopathogenic fungi. pp2-18
  6. Moulton, J. K. (1999) Studies of resistance of beet armyworm (Spodoptera exigua) to spinosad in field populations from the southern USA and southeast Asia. University of Arizona College of Agriculture 1999 Vegetable Report.
  7. Park, J. D., H. G. Goh, J. H. Lee, W. J. Kee and K. J. Kim (1991) Flight activity characteristics of beet armyworm, Spodoptera exigua (Lepidoptera : Noctuidae) in southern region of Korea. Korean. J. Appl. Entomol. 30:124-129
  8. Tabashnik, B. E. and Y. Carriere (2004) Bt transgenic crops do not have favorable effects on resistant insects. J. Insect Sci. 4:1-4.
  9. Asi, M. R., M. H. Bashir, M. Afzal, K. Zia and M. Akram (2013) Potential of entomopathogenic fungi for biocontrol of Spodoptera litura fabricius (lepidoptera : noctuidae). J. Anim. Plant Sci. 23:913-918.
  10. Petlamul, W. and P. Prasertsan (2012) Evaluation of strains of Metarhizium anisopliae and Beauveria bassiana against Spodoptera litura on the basis of their virulence, germination rate, conidia production, radial growth and enzyme activity. Kor. J. Mycobiol. 40:111-116. https://doi.org/10.5941/MYCO.2012.40.2.111
  11. Anand, R., B. Prasad and B. N. Tiwary (2009) Relative susceptibility of Spodoptera litura pupae to selected entomopathogenic fungi. BioControl 54:85-92. https://doi.org/10.1007/s10526-008-9157-x
  12. Wraight, S. P., T. M. Butt, S. Galaini-Wraight, L. L. Allee and R. S. Soper (1990) Germination and infection processes of the entomophthoralean fungus Erynia radicans on the potato leafhopper, Empoasca fabae. J. Invert. Pathol. 56: 157-174. https://doi.org/10.1016/0022-2011(90)90098-Q
  13. Hajek, A. E. and R. J. St. Leger (1994) Interaction between fungal pathogens and insect hosts. Ann. Rev. Entomol. 39: 293-322. https://doi.org/10.1146/annurev.en.39.010194.001453

피인용 문헌

  1. 흰점박이꽃무지 사육 환경에 따른 곤충 병원성 곰팡이 Metarhizium anisopliae의 병원성 vol.53, pp.1, 2014, https://doi.org/10.11614/ksl.2020.53.1.073
  2. Insecticidal Effect of Entomopathogenic fungus, Isaria fumosorosea FG340 to Thrips palmi vol.24, pp.4, 2014, https://doi.org/10.7585/kjps.2020.24.4.374