KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Isolation and Identification of Anthocyanins and Determination of Antioxidative Activity in Rice-hull of a new Cultivar, 'Jungmo1020'

'중모1020' 왕겨의 안토시아닌 동정 및 항산화 효과 검정

  • 나지은 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 박지영 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 서우덕 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 송유천 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 조준현 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 이종희 (농촌진흥청 연구정책국) ;
  • 심은영 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 남민희 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 정일민 (건국대학교 응용생물과학과) ;
  • 한상익 (농촌진흥청 국립식량과학원 기능성작물부)
  • Received : 2014.06.20
  • Accepted : 2014.09.01
  • Published : 2014.12.31
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Abstract

Anthocyanins are water-soluble plant pigments that give rise to the red, purple, or blue colors observed in many crops. Especially purple pigment of colored rice represent biological activities has been an increased interest, due to the functional meal as a staple food. These health benefits have been attributed to antioxidant property of anthocyanin. However, there have been little genetic source for development of new and various colored rice variety. A recently developed new variety, 'Jungmo1020', showed an unique characteristic of blue color of rice grain hull. We identified the pigment petunidin-3-O-glucoside (Pt-3-G) as a major compound with a value of $176.3{\pm}2.3mg/100g$ (68.3%) from 80% MeOH with 1% HCl extracts by UPLC/MS/MS. The content of Pt-3-G in the extracts using a solvent of 100% MeOH with 1% HCl was the highest with the values of $183.8{\pm}2.5mg/100g$. In addition, these extracts showed excellent antioxidatative activities by DPPH and ABTs assay.

새로 개발된 유색미 품종인 중모1020의 왕겨를 이용한 색소의 조성과 함량을 분석하고 생리활성을 검정한 결과, 중모1020의 왕겨로부터 최초로 페튜니딘 색소를 다량함유하고 있음을 Mass와 UPLC등의 기기분석을 통해 동정하였다. 중모1020의 색소 조성 중 C-3-G 함량은 29.6%인데 비해 Pt-3-G의 함량이 68.3%를 차지하여 C-3-G 함량이 82.3%인 기존의 벼 색소의 조성과는 완전히 다름을 보였다. 추출용매 조건에 따른 분석 결과, 조추출물 수율은 100% 메탄올에 1.0% HCl을 이용하였을 경우 수율이 11.4%로서 가장 높음을 보였고, 다음이 80% 메탄올에 1.0% HCl용매로 추출한 경우였다. 안토시아닌 색소 추출량 역시 1.0% HCl을 함유한 100% 메탄올과 80% 메탄올에서 각각 $275.0{\pm}4.1$, $258.0{\pm}1.8mg/100g$으로 가장 많았다. 항산화 활성 비교에서는 전체 안토시아닌 색소함량이 많고 폴리페놀 추출율이 높은 1.0% HCl을 함유한 80% 메탄올 용매 추출물에서 가장 항산화 활성이 높음을 보였다.

Keywords

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