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Total sugar contents of edible and medicinal mushrooms comparative analysis by the extraction method

추출방법별 식용 및 약용버섯류의 총 당함량 비교분석

  • Cho, Jae-Han (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Park, Hye-Sung (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Han, Jae-Gu (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Lee, Gang-Hyo (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Sung, Gi-Ho (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Jhune, Chang-Sung (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA)
  • 조재한 (국립원예특작과학원 버섯과) ;
  • 박혜성 (국립원예특작과학원 버섯과) ;
  • 한재구 (국립원예특작과학원 버섯과) ;
  • 이강효 (국립원예특작과학원 버섯과) ;
  • 성기호 (국립원예특작과학원 버섯과) ;
  • 전창성 (국립원예특작과학원 버섯과)
  • Received : 2014.11.05
  • Accepted : 2014.12.22
  • Published : 2014.12.31

Abstract

This study was to comparative analysis that different methods of extraction about total sugar content which the major functional components of edible and medicinal mushrooms. Through the phenol-sulfuric acid method was measured that Hot water extraction, acid extraction, and alkaline extraction. Drawing the standard curve to obtain the concentration of the glucose solution and the sample by applying the measured absorbance on the standard curve. Total sugar contents was measured after extraction with different solvents.(Hot water, 70% EtOH, 80% MeOH). The level of total sugar in Cordyceps militaris is highest in the hot water extraction with 658.4(${\mu}g/g$) and Coriolus versicolor in 649.7(${\mu}g/g$) and 679.0(${\mu}g/g$) was found as the highest in the acid extraction and the alkaline extraction. C. militaris and ASI 7025 showed the highest level in the hot water extraction. Finally, C. versicolor, Sparassis crispa showed that higher than the other mushrooms at the 1hr, 2hr microwave extraction. It was varied total sugar content according to the different extraction solvent and the extraction method.

이번 연구에서는 식용 및 약용버섯 자실체를 가지고 버섯의 주요 기능성 성분인 총 당의 함량을 추출방법을 달리하여 비교분석하였다. 열수추출, 산추출, 알칼리 추출을 통해 총 당 측정법으로 미량의 시료에 적합한 페놀-황산법으로 측정하였다. 표준 곡선은 glucose solution을 사용하여 작성하고 측정한 흡광도를 표준곡선에 적용하여 시료의 농도를 얻었다. 열수추출에서는 동충하초가 658.4(${\mu}g/g$)로 가장 높았고, 산추출과 알칼리 추출에서는 운지버섯이 각각 649.7(${\mu}g/g$)와 679.0(${\mu}g/g$)로 가장 높음을 알 수 있었다. 추출방법별로 열수추출과 마이크로웨이브 추출로 총 당 함량을 정량한 결과 열수 추출에서는 동충하초, ASI 7025가 높았고, 1 hr 마이크로웨이브 추출 및 2 hr 마이크로웨이브 추출에서는 운지버섯, 꽃송이가 높게 나타났다. 이러한 결과를 통해 각기 다른 추출용매와 추출방법에 따라서 추출되는 총 당 함량이 다름을 알 수 있었다.

Keywords

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