Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Phylegenetic analysis of Hericium species based on ITS rDNA sequences

ITS 분석을 이용한 노루궁뎅이버섯 수집균주의 계통분류

  • Moon, JiWon (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Develeopment Administration) ;
  • Lee, Chan-Jung (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Develeopment Administration) ;
  • Cheong, Jong-Chun (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Develeopment Administration) ;
  • Suh, Jang-Sun (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Develeopment Administration) ;
  • Kong, Won-Sik (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Develeopment Administration)
  • 문지원 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) ;
  • 이찬중 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) ;
  • 정종천 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) ;
  • 서장선 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) ;
  • 공원식 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과)
  • Received : 2014.11.10
  • Accepted : 2014.11.17
  • Published : 2014.12.31
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Abstract

The aim of this study was to analyze the genetic diversity of Hericium species based on their rDNA ITS sequences. Hericium species were collected from various regions and the size of the ITS rRNA gene regions from different Hericium species varied from 450 to 500 bp. A phylogenetic trees based on the ITS region revealed that Hericium species could be classified into 4 different groups, H. erinaceus, H. coralloide, H. alpestre, H. americanum. Among them, ASI 48015 and ASI 48016 was identified as Sprassis and Lentinula genus, respectively, based on blast searches using their rDNA ITS sequences.

수집한 46개의 균주를 배양하여 rDNA의 ITS 염기서열 분석으로 유전적 계통분류 한 결과, Heiricum속이 아닌 균주가 2균주가 있었음을 확인하였다. 본 결과를 바탕으로 효능이 있는 것으로 밝혀진 H.erinaceus와 가까운 유연관계를 지니는 종들을 위주로 계통별로 균사 생장실험, 자실체 발생조사 등을 연구할 예정이다. 또한 H.erinaceus은 아니지만, 생리활성효능이 뛰어난 종의 유무를 테스트하여 기존의 품종보다 효능 면에서 뛰어난 균주를 선발 교잡할 예정이다. 이와 같은 연구를 통해 우수품종을 선발하여 최적 환경조건 테스트까지 거친다면 노루궁뎅이 버섯 소비시장 구축에 도움이 되리라 생각된다.

Keywords

References

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