Journal of Plant Biotechnology

  • 제41권4호
  • /
  • Pages.201-205
  • /
  • 2014
  • /
  • 1229-2818(pISSN)
  • /
  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

RAW264.7 대식세포에서 풀솜대 추출물의 nitric oxide 및 prostaglandin E2생성 저해효과

Inhibitory effects of extracts from Smilacina japonica on lipopolysaccharide induced nitric oxide and prostaglandin E2 production in RAW264.7 macrophages

  • 남정환 (농촌진흥청, 국립식량과학원, 고령지농업연구센터) ;
  • 서종택 (농촌진흥청, 국립식량과학원, 고령지농업연구센터) ;
  • 김율호 (농촌진흥청, 국립식량과학원, 고령지농업연구센터) ;
  • 김기덕 (농촌진흥청, 국립식량과학원, 고령지농업연구센터) ;
  • 유동림 (농촌진흥청, 국립식량과학원, 고령지농업연구센터) ;
  • 이종남 (농촌진흥청, 국립식량과학원, 고령지농업연구센터) ;
  • 홍수영 (농촌진흥청, 국립식량과학원, 고령지농업연구센터) ;
  • 김수정 (농촌진흥청, 국립식량과학원, 고령지농업연구센터) ;
  • 손황배 (농촌진흥청, 국립식량과학원, 고령지농업연구센터) ;
  • 김현삼 (농촌진흥청, 국립식량과학원, 고령지농업연구센터) ;
  • 김보성 (농촌진흥청, 국립식량과학원, 고령지농업연구센터) ;
  • 이경태 (경희대학교, 약학대학, 약학과) ;
  • 박희준 (상지대학교, 보건대학, 제약공학과)
  • Nam, Jung-Hwan (Highland Agricultre Research Center, National Institute of Crop Science, RDA) ;
  • Seo, Jong-Taek (Highland Agricultre Research Center, National Institute of Crop Science, RDA) ;
  • Kim, Yul-Ho (Highland Agricultre Research Center, National Institute of Crop Science, RDA) ;
  • Kim, Ki-Deog (Highland Agricultre Research Center, National Institute of Crop Science, RDA) ;
  • Yoo, Dong-Lim (Highland Agricultre Research Center, National Institute of Crop Science, RDA) ;
  • Lee, Jong-Nam (Highland Agricultre Research Center, National Institute of Crop Science, RDA) ;
  • Hong, Su-Young (Highland Agricultre Research Center, National Institute of Crop Science, RDA) ;
  • Kim, Su-Jeong (Highland Agricultre Research Center, National Institute of Crop Science, RDA) ;
  • Sohn, Hwang-Bae (Highland Agricultre Research Center, National Institute of Crop Science, RDA) ;
  • Kim, Hyun-Sam (Highland Agricultre Research Center, National Institute of Crop Science, RDA) ;
  • Kim, Bo-Sung (Highland Agricultre Research Center, National Institute of Crop Science, RDA) ;
  • Lee, Kyung-Tea (Dept. of pharmaceutical, College of Pharmacy, Kyung-Hee University) ;
  • Park, Hee-Jhun (Dept. of Pharmaceutical Engineering, College of Health Sciences, Sang-ji University)
  • 투고 : 2014.09.22
  • 심사 : 2014.10.20
  • 발행 : 2014.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구에서는 풀솜대(Smilacina japonica)의 전초를 이용하여 세포독성 및 항염증 활성 효과를 평가하였다. 대식세포인 RAW264.7 cell에서 염증 매개 물질인 lipopo-lysacchride (LPS)로 염증을 유발시켜 nitric oxide (NO)와 prostaglandin $E_2$ ($PGE_2$) 같은 염증 유발인자들의 억제효과를 확인하였다. 풀솜대 chloroform 분획물의 염증 유발인자 억제 시 $IC_{80}$ value를 측정하였을 때 nitric oxide 및 prostaglandin $E_2$ 생성을 농도의존적으로 현저하게 저해하는 농도는 각각 53.3과 $32.5{\mu}g/ml$이었다. 따라서 본 연구 결과는 풀솜대의 chloroform과 같은 비극성용매 분획물들이 유의성 있는 항염증 효과를 나타내었으며, 이러한 효능은 예방의학적 가능성을 충분히 가지고 있기에 염증성질환의 예방을 위한 건강 기능성식품의 개발 가능성을 제시할 수 있을 것으로 기대된다. 또한 염증과 관련된 사이토카인 및 단백질 발현 메커니즘에 대한 추가적인 연구가 필요할 것으로 판단된다.

Smilacina japonica is a localized common rhizomatous flowering plant, This plant is often used in Korean traditional systems of medicine as a remedy for migrain, diplegia, physical impurity, blood circulation, abscess and contusion. Generally drugs that are used for arthritis have antinociceptive and anti-inflammatory properties. However, validity of the anti-inflammatory activity has not been scientifically investigated so far. Therefore, the aim of this study was to investigate the anti-inflammatory potential of S. japonica using the ethanolic extract and its subfractions. To evaluate the anti-inflammatory effects, we examined the inflammatory mediators such as nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) on RAW 264.7 macrophages. Our results indicated that hexane fraction significantly inhibited the LPS induced NO and $PGE_2$ production in the cells. The hexane fractions inhibitory activity for NO tests with $IC_{50}$ values showed in $53.3{\mu}g/ml$ and $PGE_2$ tests with $IC_{50}$ values showed at $32.5{\mu}g/ml$. Theseis result suggest a potential role of hexane fraction from S. japonica as source of anti-inflammatory agent.

키워드

참고문헌

  1. Bak MJ, Jeong JH, Kang HS, Jin KS, Seon OK, Jeong WS(2009) Cedrela sinensis leaves suppress oxidative stress and expressions of iNOS and COX-2 via MAPK signaling pathways in RAW 264.7 cells J Food Sci Nutr 14:269-276 https://doi.org/10.3746/jfn.2009.14.4.269
  2. Cho JH, Choi GH, Park IJ, Baik SO, Kim HH, Kim CS(2014) Development of Functional food materials from Acanthopanax senticosus fermented mushroom mycelia. J Korean Soc Food Sci Nutr 43:411-418 https://doi.org/10.3746/jkfn.2014.43.3.411
  3. Cheon YP, Mohammad LM, Park CH, Hong JH, Lee GD, Song JC, Kim KS(2009) Bulnesia sarmienti aqueous extract inhibits inflammation in LPS-stimulated RAW264.7cells. J Life Sci 19:479-485 https://doi.org/10.5352/JLS.2009.19.4.479
  4. Feldmann M, Brennan FM, Maini RN(1996) Role of cytokines in Rheumatoid arthritis. Annu Rev Immunol 14:397-440 https://doi.org/10.1146/annurev.immunol.14.1.397
  5. Hilliquin P, Borderie D, Hernvann A, Menkes CJ, Ekindjian OG(1997) Nitric oxide as S-nitrosoproteins in Rheumatoidarthritis. Arthritis Rheum 40:1512-1517 https://doi.org/10.1002/art.1780400820
  6. Hwang IG, Kim HY, Shin SL, Lee CH, Lee JS, Jang KI, Jeong HS(2010) Biological activities of Coreopsistinctoria Nutt. Flower extracts. Kor J Hort Sci 28:857-863
  7. Iontcheva I, Amar S, Zawawi KH, Kantarci A, VanDyke TE(2004) Role for moesin in lipopolysaccharide stimulated signal transduction. Infect Immun 72:2312-2320 https://doi.org/10.1128/IAI.72.4.2312-2320.2004
  8. Karin M, Ben-Neriah Y(2000) Phosphorylation meetsubiquitination: The control of NF-B activity. Annu Rev Immunol 18:621-663 https://doi.org/10.1146/annurev.immunol.18.1.621
  9. Kim KA, Han JS, Cheon KS, Park YH, Kang JS, Yoo KO(2014) Floristic study of Mt. Dosol and its adjacent areas. Korean J Pl Taxon 44:59-76 https://doi.org/10.11110/kjpt.2014.44.1.59
  10. Kim YS, Lee SJ, Hwang JW, Kim EH, Park PJ, Jeong JH(2012) Anti-inflammatory effects of extracts from Ligustrum ovalifolium H. leaves on RAW264.7 macrophages. J Korean Soc Food Sci Nutr 4:1205-1210 https://doi.org/10.3746/jkfn.2012.41.9.1205
  11. Lee BG, Kim JH, Ham SG, Lee CE(2014) Study on biological activities of extracts for cosmeceutical development from Lagerstroemia indica L. Branch. Korean J Plant Res 27:29-34 https://doi.org/10.7732/kjpr.2014.27.1.029
  12. Nava E, Palmer RM, Moncada S(1992) The role of metric oxide in endotoxic shock: effects of NG-monomethyl-L-arginine. J Cardiovasc Pharmacol 12:132-134
  13. Nishida T, Yabe Y, Fu HY, Hayashi Y, Asahi K, Eguchi H, Tsuji S, Tsujii M, Hayas-hi N, Kawano S(2007) Geranylgeranylacetone induces cyclooxygenase-2 expression in cultured rat gastric epithelial cells through NF-$\kappa{B}$. Dig Dis Sci 52:1890-1896 https://doi.org/10.1007/s10620-006-9661-8
  14. Park HW, Baek NI, Kim SH, Kwon BM, Chung IS, Park MH, Kim SH, Kim DK(2004) Phytochemical components from the whole plants of Arabis glabra(L.) Bernh. Kor J Pharmacogn 35:320-323
  15. Uttara B, Singh AV, Zamboni P, Mahajan RT(2009) Oxidative stress and neurodednergeat ive iseases : a review of upstream and downstream antioxidant therapeutic options. Curr Neuropharmacol 7:65-74 https://doi.org/10.2174/157015909787602823
  16. Yoo KO, Lee WT, Oh YJ(1999) External morphology and vegetation of Megaleranthis saniculifolia populations in four different habitats. Korean J Plant Res 12:312-323
  17. Yun CH, Shin JS, Park HJ, Park JH, Lee KT(2010) Inhibition of LPS-induced iNOS, COX-2 expression and cytokines production by fupenjic acid in macrophage Cells. Kor J Pharmacogn 41:14-20

피인용 문헌

  1. Inhibitory effect ofAralia elataethanol extract against skin damage in UVB-exposed human keratinocytes and human dermal fibroblasts vol.49, pp.6, 2016, https://doi.org/10.4163/jnh.2016.49.6.429