Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Inhibitory effects of extracts from Smilacina japonica on lipopolysaccharide induced nitric oxide and prostaglandin E2 production in RAW264.7 macrophages

RAW264.7 대식세포에서 풀솜대 추출물의 nitric oxide 및 prostaglandin E2생성 저해효과

  • Nam, Jung-Hwan (Highland Agricultre Research Center, National Institute of Crop Science, RDA) ;
  • Seo, Jong-Taek (Highland Agricultre Research Center, National Institute of Crop Science, RDA) ;
  • Kim, Yul-Ho (Highland Agricultre Research Center, National Institute of Crop Science, RDA) ;
  • Kim, Ki-Deog (Highland Agricultre Research Center, National Institute of Crop Science, RDA) ;
  • Yoo, Dong-Lim (Highland Agricultre Research Center, National Institute of Crop Science, RDA) ;
  • Lee, Jong-Nam (Highland Agricultre Research Center, National Institute of Crop Science, RDA) ;
  • Hong, Su-Young (Highland Agricultre Research Center, National Institute of Crop Science, RDA) ;
  • Kim, Su-Jeong (Highland Agricultre Research Center, National Institute of Crop Science, RDA) ;
  • Sohn, Hwang-Bae (Highland Agricultre Research Center, National Institute of Crop Science, RDA) ;
  • Kim, Hyun-Sam (Highland Agricultre Research Center, National Institute of Crop Science, RDA) ;
  • Kim, Bo-Sung (Highland Agricultre Research Center, National Institute of Crop Science, RDA) ;
  • Lee, Kyung-Tea (Dept. of pharmaceutical, College of Pharmacy, Kyung-Hee University) ;
  • Park, Hee-Jhun (Dept. of Pharmaceutical Engineering, College of Health Sciences, Sang-ji University)
  • 남정환 (농촌진흥청, 국립식량과학원, 고령지농업연구센터) ;
  • 서종택 (농촌진흥청, 국립식량과학원, 고령지농업연구센터) ;
  • 김율호 (농촌진흥청, 국립식량과학원, 고령지농업연구센터) ;
  • 김기덕 (농촌진흥청, 국립식량과학원, 고령지농업연구센터) ;
  • 유동림 (농촌진흥청, 국립식량과학원, 고령지농업연구센터) ;
  • 이종남 (농촌진흥청, 국립식량과학원, 고령지농업연구센터) ;
  • 홍수영 (농촌진흥청, 국립식량과학원, 고령지농업연구센터) ;
  • 김수정 (농촌진흥청, 국립식량과학원, 고령지농업연구센터) ;
  • 손황배 (농촌진흥청, 국립식량과학원, 고령지농업연구센터) ;
  • 김현삼 (농촌진흥청, 국립식량과학원, 고령지농업연구센터) ;
  • 김보성 (농촌진흥청, 국립식량과학원, 고령지농업연구센터) ;
  • 이경태 (경희대학교, 약학대학, 약학과) ;
  • 박희준 (상지대학교, 보건대학, 제약공학과)
  • Received : 2014.09.22
  • Accepted : 2014.10.20
  • Published : 2014.12.31
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Abstract

Smilacina japonica is a localized common rhizomatous flowering plant, This plant is often used in Korean traditional systems of medicine as a remedy for migrain, diplegia, physical impurity, blood circulation, abscess and contusion. Generally drugs that are used for arthritis have antinociceptive and anti-inflammatory properties. However, validity of the anti-inflammatory activity has not been scientifically investigated so far. Therefore, the aim of this study was to investigate the anti-inflammatory potential of S. japonica using the ethanolic extract and its subfractions. To evaluate the anti-inflammatory effects, we examined the inflammatory mediators such as nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) on RAW 264.7 macrophages. Our results indicated that hexane fraction significantly inhibited the LPS induced NO and $PGE_2$ production in the cells. The hexane fractions inhibitory activity for NO tests with $IC_{50}$ values showed in $53.3{\mu}g/ml$ and $PGE_2$ tests with $IC_{50}$ values showed at $32.5{\mu}g/ml$. Theseis result suggest a potential role of hexane fraction from S. japonica as source of anti-inflammatory agent.

본 연구에서는 풀솜대(Smilacina japonica)의 전초를 이용하여 세포독성 및 항염증 활성 효과를 평가하였다. 대식세포인 RAW264.7 cell에서 염증 매개 물질인 lipopo-lysacchride (LPS)로 염증을 유발시켜 nitric oxide (NO)와 prostaglandin $E_2$ ($PGE_2$) 같은 염증 유발인자들의 억제효과를 확인하였다. 풀솜대 chloroform 분획물의 염증 유발인자 억제 시 $IC_{80}$ value를 측정하였을 때 nitric oxide 및 prostaglandin $E_2$ 생성을 농도의존적으로 현저하게 저해하는 농도는 각각 53.3과 $32.5{\mu}g/ml$이었다. 따라서 본 연구 결과는 풀솜대의 chloroform과 같은 비극성용매 분획물들이 유의성 있는 항염증 효과를 나타내었으며, 이러한 효능은 예방의학적 가능성을 충분히 가지고 있기에 염증성질환의 예방을 위한 건강 기능성식품의 개발 가능성을 제시할 수 있을 것으로 기대된다. 또한 염증과 관련된 사이토카인 및 단백질 발현 메커니즘에 대한 추가적인 연구가 필요할 것으로 판단된다.

Keywords

References

  1. Bak MJ, Jeong JH, Kang HS, Jin KS, Seon OK, Jeong WS(2009) Cedrela sinensis leaves suppress oxidative stress and expressions of iNOS and COX-2 via MAPK signaling pathways in RAW 264.7 cells J Food Sci Nutr 14:269-276 https://doi.org/10.3746/jfn.2009.14.4.269
  2. Cho JH, Choi GH, Park IJ, Baik SO, Kim HH, Kim CS(2014) Development of Functional food materials from Acanthopanax senticosus fermented mushroom mycelia. J Korean Soc Food Sci Nutr 43:411-418 https://doi.org/10.3746/jkfn.2014.43.3.411
  3. Cheon YP, Mohammad LM, Park CH, Hong JH, Lee GD, Song JC, Kim KS(2009) Bulnesia sarmienti aqueous extract inhibits inflammation in LPS-stimulated RAW264.7cells. J Life Sci 19:479-485 https://doi.org/10.5352/JLS.2009.19.4.479
  4. Feldmann M, Brennan FM, Maini RN(1996) Role of cytokines in Rheumatoid arthritis. Annu Rev Immunol 14:397-440 https://doi.org/10.1146/annurev.immunol.14.1.397
  5. Hilliquin P, Borderie D, Hernvann A, Menkes CJ, Ekindjian OG(1997) Nitric oxide as S-nitrosoproteins in Rheumatoidarthritis. Arthritis Rheum 40:1512-1517 https://doi.org/10.1002/art.1780400820
  6. Hwang IG, Kim HY, Shin SL, Lee CH, Lee JS, Jang KI, Jeong HS(2010) Biological activities of Coreopsistinctoria Nutt. Flower extracts. Kor J Hort Sci 28:857-863
  7. Iontcheva I, Amar S, Zawawi KH, Kantarci A, VanDyke TE(2004) Role for moesin in lipopolysaccharide stimulated signal transduction. Infect Immun 72:2312-2320 https://doi.org/10.1128/IAI.72.4.2312-2320.2004
  8. Karin M, Ben-Neriah Y(2000) Phosphorylation meetsubiquitination: The control of NF-B activity. Annu Rev Immunol 18:621-663 https://doi.org/10.1146/annurev.immunol.18.1.621
  9. Kim KA, Han JS, Cheon KS, Park YH, Kang JS, Yoo KO(2014) Floristic study of Mt. Dosol and its adjacent areas. Korean J Pl Taxon 44:59-76 https://doi.org/10.11110/kjpt.2014.44.1.59
  10. Kim YS, Lee SJ, Hwang JW, Kim EH, Park PJ, Jeong JH(2012) Anti-inflammatory effects of extracts from Ligustrum ovalifolium H. leaves on RAW264.7 macrophages. J Korean Soc Food Sci Nutr 4:1205-1210 https://doi.org/10.3746/jkfn.2012.41.9.1205
  11. Lee BG, Kim JH, Ham SG, Lee CE(2014) Study on biological activities of extracts for cosmeceutical development from Lagerstroemia indica L. Branch. Korean J Plant Res 27:29-34 https://doi.org/10.7732/kjpr.2014.27.1.029
  12. Nava E, Palmer RM, Moncada S(1992) The role of metric oxide in endotoxic shock: effects of NG-monomethyl-L-arginine. J Cardiovasc Pharmacol 12:132-134
  13. Nishida T, Yabe Y, Fu HY, Hayashi Y, Asahi K, Eguchi H, Tsuji S, Tsujii M, Hayas-hi N, Kawano S(2007) Geranylgeranylacetone induces cyclooxygenase-2 expression in cultured rat gastric epithelial cells through NF-$\kappa{B}$. Dig Dis Sci 52:1890-1896 https://doi.org/10.1007/s10620-006-9661-8
  14. Park HW, Baek NI, Kim SH, Kwon BM, Chung IS, Park MH, Kim SH, Kim DK(2004) Phytochemical components from the whole plants of Arabis glabra(L.) Bernh. Kor J Pharmacogn 35:320-323
  15. Uttara B, Singh AV, Zamboni P, Mahajan RT(2009) Oxidative stress and neurodednergeat ive iseases : a review of upstream and downstream antioxidant therapeutic options. Curr Neuropharmacol 7:65-74 https://doi.org/10.2174/157015909787602823
  16. Yoo KO, Lee WT, Oh YJ(1999) External morphology and vegetation of Megaleranthis saniculifolia populations in four different habitats. Korean J Plant Res 12:312-323
  17. Yun CH, Shin JS, Park HJ, Park JH, Lee KT(2010) Inhibition of LPS-induced iNOS, COX-2 expression and cytokines production by fupenjic acid in macrophage Cells. Kor J Pharmacogn 41:14-20

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