Advances in materials Research

Techno-Press (테크노프레스)
권호 표지
DOI QR코드

DOI QR Code

Radiation induced synthesis of (gelatin-co-PVA)-g-poly (AAc) copolymer as wound dressing material

  • Kaur, Inderjeet (Department of Chemistry, Himachal Pradesh University Summer Hill) ;
  • Bhati, Pooja (Department of Chemistry, Himachal Pradesh University Summer Hill) ;
  • Sharma, Sushma (Department of Bio sciences, Himachal Pradesh University Summer Hill)
  • Received : 2014.02.28
  • Accepted : 2014.12.16
  • Published : 2014.12.25
⟨ Previous Next ⟩

Abstract

Copolymers of gelatin and poly (vinyl alcohol), (PVA) grafted by acrylic acid (AAc) with excellent water absorption and retention abilities under neutral conditions were successfully synthesized using $^{60}Co$ gamma radiations in presence of ammonium persulphate (APS), as water soluble initiator and sodium bicarbonate ($NaHCO_3$) as foaming agent. The optimum synthesis conditions pertaining to maximum swelling percentage were evaluated as a function of gelatin/PVA ratio, amount of water, concentration of APS, $NaHCO_3$, monomer concentration and total irradiation dose. Maximum percent swelling (1694.59%) of the copolymer, gelatin-co-PVA, was obtained at optimum $[APS]=2.92{\times}10^{-1}mol/L$, $[NaHCO_3]=7.94{\times}10^{-2}mol/L$ and 1.5 mL of water at total dose of 31.104 kGy while in case of grafted copolymer, (gelatin-co-PVA)-g-poly(AAc), maximum percent swelling (560.86%) was obtained using $8.014{\times}10^{-1}mol/L$ of AAc in 9 mL water with 31.104 kGy preirradiation dose. The pristine and grafted copolymers were characterized by Fourier Transform Infrared Spectroscopy (FTIR), Scanning electron Microscopy (SEM), Thermal gravimetric analysis (TGA) and X-Ray Diffraction (XRD) methods. The copolymers loaded with an antiseptic, Povidone, were used as wound dressing materials for wounded gastrocnemius muscle of mice and the results exhibit that (gelatin-co-PVA)-g-poly (AAc) copolymer is a potent wound dressing material as compared to the copolymer.

Keywords

References

  1. Abed, M.A. and Bohidar, H.B. (2005), "Surfactant induced softening in gelatin hydrogels", Eur. Polym. J., 41, 2395-2405. https://doi.org/10.1016/j.eurpolymj.2005.04.027
  2. Abou Taleb, M.F., Ismail, S.A. and El- Kelesh, N.A. (2009), "Radiation synthesis and characterization of polyvinyl alcohol/methacrylic acid-gelatin hydrogel for vitro drug delivery", J. Macromol. Sci. Part A, 46, 170-178.
  3. Balakrishnan, B. and Jayakrishnan, A. (2005), "Self-cross-linking biopolymers as injectable in situ forming biodegradable scaffolds", Biomaterials, 26, 3941-3951. https://doi.org/10.1016/j.biomaterials.2004.10.005
  4. Bardajee, G.R., Pourjavadi, A. and Sheikh, N. (2008), "Grafting of acrylamide onto kappa-carrageenan via ${\gamma}$-irradiation: optimization and swelling behavior", Radiat. Phys. Chem., 77, 131-137. https://doi.org/10.1016/j.radphyschem.2007.04.004
  5. Kaur, I., Bhati, P., Bala, K. and Kanwar, S.S., (2014), "Effective immobilization of lipase onto a porous gelatin-co-poly(vinyl alcohol) copolymer and evaluation of its hydrolytic properties", J. Appl. Polym. Sci., 131, 39622.
  6. Kim, S.J., Park, S.J. and Kim, S.I. (2003), "Swelling behaviour of interpenetrating polymer network hydrogels composed of poly (vinyl alcohol) and chitosan", Reac. Funct. Polym., 55, 53-59. https://doi.org/10.1016/S1381-5148(02)00214-6
  7. Koob, T.J. and Hernandez, D.J. (2003), "Mechanical and thermal properties of novel polymerized NDGA-gelatin hydrogels", Biomaterials, 24, 1285-1292. https://doi.org/10.1016/S0142-9612(02)00465-9
  8. Kwon, O.H., Nho, Y.C. and Lee, Y.M. (2003),"Radiation induced copolymerization of 2-Hydroxyethylmethacrylate and polyethylene glycol methacrylate, and its protein adsorption and bacterial attachment", J. Ind. Eng. Chem., 9, 138-145.
  9. Nho, Y.C., Moon, S.W., Lee, K.H., Park, C.W., Suh, T.S., Jung, Y.T., Ahn, W.S., and Chun, H.J. (2005), "Evaluation of poly (vinylalcohol) hydrogels cross-linked under ${\gamma}$-ray irradiation", J. Ind. Eng. Chem., 11, 159-164.
  10. Pal, K., Banthia, A.K. and Majumdar, D.K. (2006), "Polyvinyl alcohol-gelatin patches of salicylic acid: preparation, characterization and drug release studies", J. Biomater. Appl., 21, 75-91. https://doi.org/10.1177/0885328206056312
  11. Pal, K., Banthia, A.K. and Majumdar, D.K. (2007a), "Biomedical evaluation of polyvinyl alcohol-gelatin esterified hydrogel for wound dressing", J. Mater. Sci.: Mater. Med., 18, 1889-1894. https://doi.org/10.1007/s10856-007-3061-2
  12. Pal, K., Banthia, A.K. and Majumdar, D.K. (2007), "Preparation and characterization of polyvinyl alcohol-gelatin hydrogel membranes for biomedical applications", AAPS Pharmsci. Tech. 8(1) article 21. https://doi.org/10.1208/pt0801003
  13. Park, K.R. and Nho, Y.C. (2003), "Preparation and Characterization by Radiation of poly (vinyl alcohol) and poly (n-vinylpyrrolidone) hydrogels containing aloe vera", J. Appl. Polym. Sci., 90, 1477-1485. https://doi.org/10.1002/app.12656
  14. Ruiz, J., Mantecon, A. and Cadiz, V. (2002), "Investigation of loading and release in PVA-based hydrogels", J. Appl. Poly. Sci., 85, 1644-1651. https://doi.org/10.1002/app.10696
  15. Seo, K.H., You, S.J. and Chun, H.J. (2009), "In vitro and in vivo biocompatibility of ${\gamma}$-ray crosslinked gelatin-poly (vinyl alcohol) hydrogels", Tiss. Engg. Regen. Med., 6, 414-418.
  16. Tan, H.,Wu, J. and Lao, L. (2009), "Gelatin/chitosan/hyaluronan scaffold integrated with PLGA microspheres for cartilage tissue engineering", Acta Biomater., 5, 328-337. https://doi.org/10.1016/j.actbio.2008.07.030
  17. Teramoto, Y. and Nishio, Y. (2003), "Cellulose diacetate-graft-poly (lactic acid)s: Synthesis of wide-ranging compositions and their thermal and mechanical properties", Polymer, 44, 2701-2709. https://doi.org/10.1016/S0032-3861(03)00190-3
  18. Wang, M., Li, Y. and Wu, J. (2008), "In vitro and in vivo study to the biocompatibility and biodegradation of hydroxyapatite/poly (vinyl alcohol)/gelatin composite", J. Biomed. Mater. Res., 85, 418-426.
  19. Yang, S., Fu, Y. and Jeong, S.H. (2004), "Application of poly(acrylic acid) superporous hydrogel microparticles as a super-disintegrant in fast-disintegrating tablets", J. Pharm. Pharmacol., 56, 429-436. https://doi.org/10.1211/0022357023015
  20. You, S.J., Ahn, W.S. and Jang, H.S. (2007), "Preparation and characterization of gelatin-poly(vinyl alcohol) hydrogels for three dimensional culture", J. Ind. Eng. Chem., 13, 116-120.
  21. Young, S., Wong, M. and Tabata, Y. (2005), "Gelatin as a delivery vehicle for the controlled release of bioactive molecules", J. Control. Rel., 109, 256-274. https://doi.org/10.1016/j.jconrel.2005.09.023
  22. Yung, C.W., Wu, L.Q. and Tullman, J.A. (2007), "Transglutaminase crosslinked gelatin as a tissue engineering scaffold", J. Biomed. Mater. Res., 83, 1039-1046.

Cited by

  1. A Review on Gelatin Based Hydrogels for Medical Textile Applications vol.2020, pp.None, 2020, https://doi.org/10.1155/2020/8866582
  2. Engineered Bioactive Polymeric Surfaces by Radiation Induced Graft Copolymerization: Strategies and Applications vol.13, pp.18, 2014, https://doi.org/10.3390/polym13183102