Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Apoptotic Effects of Eugenol-loaded Nanoemulsions in Human Colon and Liver Cancer Cell Lines

  • Majeed, Hamid (Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Food Science and Technology, Jiangnan University) ;
  • Antoniou, John (Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Food Science and Technology, Jiangnan University) ;
  • Fang, Zhong (Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Food Science and Technology, Jiangnan University)
  • Published : 2014.11.28
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Abstract

Background: In this study eugenol (EU) loaded nanoemulsions (NEs) emulsified with modified starch were prepared and their apoptotic potential against liver and colon cancer cells was examined in comparison with bulk EU. Materials and Methods: We prepared stable EU loaded NEs whcih were characterized by dynamic light scattering, centrifugation and gas chromatography. Furthermore, cell viability was determined using MTT assay, and apoptosis and cell cycle analysess by flow cytometry. Results: HB8065 (liver) and HTB37 (colon) cells when treated with EU:CA NEs demonstrated greater apoptotic cells percentages as evidenced by microscopic images and flow cytometric evaluations. It was observed that EU and EU:CA NE induced apoptosis in both cell lines via reactive oxygen species (ROS) generation. Conclusions: The present study demonstrated that ROS plays a critical role in EU and EU:CA NE induced apoptosis in HB8065 and HTB37 cells. This is the first report on the antiproliferative mechanisms of EU loaded NE.

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References

  1. Acosta E (2009). Bioavailability of nanoparticles in nutrient and nutraceutical delivery. Curr Opin Colloid Interface Sci, 14, 3-15. https://doi.org/10.1016/j.cocis.2008.01.002
  2. Ahmed K, Li Y, McClements DJ (2012). Nanoemulsion and emulsion based delivery systems for curcumin: Encapsulation and release properties. Food Chem, 132, 799-807. https://doi.org/10.1016/j.foodchem.2011.11.039
  3. Asha MK, Prashanth D, Murali B (2001). Anthelmintic activity of essential oil of Ocimum sanctum and eugenol. Fitoterapia, 72, 669-70. https://doi.org/10.1016/S0367-326X(01)00270-2
  4. Babich H, Stern A, Borefreund E (1993). Eugenol cytotoxicity evaluated with continuous cell lines. Toxicol Vitro, 7, 105-9. https://doi.org/10.1016/0887-2333(93)90119-P
  5. Benencia F, Courreges MC (2000). In vitro and in vivo activity of eugenol on human herpesvirus. Phytother Res, 14, 495-500. https://doi.org/10.1002/1099-1573(200011)14:7<495::AID-PTR650>3.0.CO;2-8
  6. Chen M, Chu Y, Lai P (2011). Experimental results of colorectal cancer chemoprevention by curcuminoids loaded nanocarrier drug delivery system increased in vitro biocompatibility. Dig J Nanomater Biostruct, 6, 1187-97.
  7. Chogo JB, Crank G (1981). Chemical composition and biological activity of the tanzanian plant Ocimum suave. J Nat Prod, 42, 308-11.
  8. Das RK, Kasoju N, Bora U (2010). Encapsulation of curcumin in alginate-chitosan-pluronic c composite nanoparticles for delivery to cancer cells. Nanomedicine, 6, 153-60. https://doi.org/10.1016/j.nano.2009.05.009
  9. Donsi F, Annunziata M, Vincensi M (2011). Design of nanoemulsion based delivery systems of natural antimicrobials: Effect of the emulsifier. J Biotechnol, 159, 324-50.
  10. Dwivedi V, Shrivastava R, Hussain S (2011). Comparative anticancer potential of clove (Syzgium aromaticum) an Indian spice against cancer lines of various anatomical origin. Asian Pac J Cancer Prev, 12, 1989-93.
  11. Ghosh R, Nadiminty N, Fitzpatrick JE (2005). Eugenol causes melanoma growth suppression through inhibition of E2F1 transcriptional activity. J Biol Chem, 280, 5812-9. https://doi.org/10.1074/jbc.M411429200
  12. Haggar FA, Boushey RP (2009). Colorectal cancer epidemiology: incidence, mortality, survival and risk factors. Clin Colon Rectal Surg, 22, 191-97. https://doi.org/10.1055/s-0029-1242458
  13. Jaganathan Sk, Mazumdar A, Mondhe D (2011). Apoptotic effect of eugenol in human colon cancer cell lines. Cell Biol Int, 35, 607-15. https://doi.org/10.1042/CBI20100118
  14. Jemal A, Siegel R, Ward E (2009). Global cancer statistics. CA: A Cancer J Clin, 59, 225-49. https://doi.org/10.3322/caac.20006
  15. Kabalnov A (2001). Ostwald ripening and related phenomena. J Dispersion Sci Technol, 22, 1-12. https://doi.org/10.1081/DIS-100102675
  16. Kim SS, Oh OJ, Min HY (2003). Eugenol suppresses cyclooxygenase-2 expression in lipopolysaccharide-stimulated mouse macrophage RAW264.7 cells. Life Sci, 73, 337-48. https://doi.org/10.1016/S0024-3205(03)00288-1
  17. Liang R, Xu S, Shoemaker CF (2012). Physical and antimicrobial properties of peppermint oil nanoemulsions. J Agric Food Chem, 60, 7548-55. https://doi.org/10.1021/jf301129k
  18. Manikandan P, Murugan RS, Priyadarsini RV (2010). Eugenol induces apoptosis and inhibits invasion and angiogenesis in a rat model of gastric carcinogenesis induced by MNNG. Life Sci, 86, 936-41. https://doi.org/10.1016/j.lfs.2010.04.010
  19. Moon S, Kim H, Cha J (2011). Synergistic effect between clove oil and its major compounds and antibiotics against oral bacteria. Arch Oral Biol, 56, 907-16. https://doi.org/10.1016/j.archoralbio.2011.02.005
  20. Pisano M, Pagnan G, Loi M (2007). Antiproliferative and proapoptotic activity of eugenol related biphenyls on malignant melanoma cells. Mol Cancer, 18, 6-8.
  21. Qian C, McClements DJ (2011). Formation of nanoemulsions stabilized by model food-grade emulsifiers using high-pressure homogenization: Factors affecting particle size. Food Hydrocolloids, 25, 1000-8. https://doi.org/10.1016/j.foodhyd.2010.09.017
  22. Srivastava Rk, Chen Q, Siddiqui I (2007). Linkage of curcumin-induced cell cycle arrest and apoptosis by cyclin-dependent kinase inhibitor p21/WAF1/CIP1. Cell Cycle, 6, 2953-61. https://doi.org/10.4161/cc.6.23.4951
  23. Tan T, Tsal H, Lu H (2006). Curcumin-induced cell cycle arrest and apoptosis in human acute promyelocytic leukemia HL-60 Cells via MMP changes and caspase-3 activation. Anticancer Res, 26, 4361-72.
  24. Terjung N, Loeffler M, Gibis M (2012). Influence of droplet size on the efficacy of oil-in-water emulsions loaded with phenolic antimicrobials. Food Funct, 3, 290-301. https://doi.org/10.1039/C2FO10198J
  25. Thangapazham RL, Puri A, Tele S (2008). Evaluation of a nanotechnology-based carrier for delivery of curcumin in prostate cancer cells. Int J Oncol, 32, 1119-23.
  26. Wang XY, Wang YW, Jiang Y (2008). Enhancing bioavailability of curcumin through O/W nanoemulsions. Food Chem, 108, 419-24. https://doi.org/10.1016/j.foodchem.2007.10.086
  27. William GT (1991). Programmed cell death. Apoptosis and oncogenesis. Cell, 65, 1097-98. https://doi.org/10.1016/0092-8674(91)90002-G
  28. Yang C, Shen H, Ong C (2000). Ebselen induces apoptosis in HepG2 cells through rapid depletion of intracellular thiols. Arch Biochem Biophys, 374, 142-52. https://doi.org/10.1006/abbi.1999.1574
  29. Yoo CB, Han KT, Cho KS (2005). Eugenol isolated from the essential oil of Eugenia caryophyllata induces a reactive oxygen species-mediated apoptosis in HL-60 human promyelocytic leukemia cells. Cancer Lett, 225, 41-52. https://doi.org/10.1016/j.canlet.2004.11.018
  30. Zheng GQ, Kenney PM, Lam LKT (1992). Sesquiterpenes from clove (Eugenia caryophyllata). J Nat Prod, 55, 999-1003. https://doi.org/10.1021/np50085a029

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