Asian Pacific Journal of Cancer Prevention

  • 제15권22호
  • /
  • Pages.9997-10001
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  • 2014
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  • 1513-7368(pISSN)
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  • 2476-762X(eISSN)
아시아태평양암예방학회 (Asian Pacific Journal of Cancer Prevention)
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Curdione Inhibits Proliferation of MCF-7 Cells by Inducing Apoptosis

  • Li, Juan (Department of Oncology, Second Affiliated Hospital of Nanjing Medical University) ;
  • Bian, Wei-He (Department of Oncology, First Clinical Medicine School, Nanjing University of Chinese Medicine) ;
  • Wan, Juan (Department of Oncology, Second Affiliated Hospital of Nanjing Medical University) ;
  • Zhou, Jing (Taizhou People's Hospital) ;
  • Lin, Yan (Department of Oncology, Second Affiliated Hospital of Nanjing Medical University) ;
  • Wang, Ji-Rong (Department of Oncology, Second Affiliated Hospital of Nanjing Medical University) ;
  • Wang, Zhao-Xia (Department of Oncology, Second Affiliated Hospital of Nanjing Medical University) ;
  • Shen, Qun (Department of Hematology, First Clinical Medicine School, Nanjing University of Chinese Medicine) ;
  • Wang, Ke-Ming (Department of Oncology, Second Affiliated Hospital of Nanjing Medical University)
  • 발행 : 2014.12.18
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초록

Background: Curdione, one of the major components of Curcuma zedoaria, has been reported to possess various biological activities. It thus might be a candidate anti-flammatory and cancer chemopreventive agent. However, the precise molecular mechanisms of action of curdione on cancer cells are still unclear. In this study, we investigated the effect of curdione on breast cancer. Materials and Methods: Xenograft nude mice were used to detect the effect of curdione on breast cancer in vivo; we also tested the effect of curdione on breast cancer in vitro by MTT, Flow cytometry, JC-I assay, and western blot. Results: Firstly, we found that curdione significantly suppressed tumor growth in a xenograft nude mouse breast tumor model in a dose-dependent manner. In addition, curdione treatment inhibited cell proliferation and induced cell apoptosis. Moreover, after curdione treatment, increase of impaired mitochondrial membrane potential occurred in a concentration dependent manner. Furthermore, the expression of apoptosis-related proteins including cleaved caspase-3, caspase-9 and Bax was increased in curdione treatment groups, while the expression of the anti-apoptotic Bcl-2 was decreased. Inhibitors of caspase-3 were used to confirm that curdione induced apoptosis. Conclusions: Overall, our observations first suggested that curdione inhibited the proliferation of breast cancer cells by inducing apoptosis. These results might provide some molecular basis for the anti-cancer activity of curdione.

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참고문헌

  1. Desagher S, Martinou JC (2000). Mitochondria as the central control point of apoptosis. Trends Cell Biol, 10, 369-77. https://doi.org/10.1016/S0962-8924(00)01803-1
  2. Dohare P, Garg P, Sharma U, et al (2008). Neuroprotective efficacy and therapeutic window of curcuma oil: in rat embolic stroke model. BMC Complement. Altern Med, 8, 55-74.
  3. Ewings KE, Wiggins CM, Cook SJ (2007). Bim and the prosurvival Bcl-2 proteins:opposites attract, ERK repels. Cell Cycle, 6, 2236-40. https://doi.org/10.4161/cc.6.18.4728
  4. Green DR, Chipuk JE (2008). Apoptosi s: stabbed in the BAX. Nature, 455, 1047-9. https://doi.org/10.1038/4551047a
  5. Hikino H, Sakurai Y, Takahashi H, et al (1967). Structure of curdione. Chem Pharm Bull, 15, 1390. https://doi.org/10.1248/cpb.15.1390
  6. Hishikawa K, Nakaki T, Fujii T (2000). Connective tissue growth factor induces apoptosis via caspase-3 in cultured human aortic smooth muscle cells. Eur J Pharmacol, 392, 19-22. https://doi.org/10.1016/S0014-2999(00)00115-1
  7. Jemal A, Bray F, Center MM, et al (2011). Global cancer statistics. CA Cancer J Clin, 61, 69-90. https://doi.org/10.3322/caac.20107
  8. Jing GJ, Wang JJ, Zhang SX (2012). ER stress and apoptosis:a new mechanism for retinal cell death. Experimental Diabetes Research, Volume Article ID 589589, 11.
  9. Kaufmann SH, Lee SH, Meng XW, et al (2008). Apoptosis is associated caspase activation assays. Methods, 44, 262-72. https://doi.org/10.1016/j.ymeth.2007.11.005
  10. Kerr JF, Wyllie AH, Currie AR (1972). Apoptosis:a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer, 26, 239-57. https://doi.org/10.1038/bjc.1972.33
  11. Kuribayashi K, Mayes PA, El-Deiry WS (2006). What are caspases 3 and 7 doing upstream of the mitochondria? Cancer Biol Ther, 5, 763-5. https://doi.org/10.4161/cbt.5.7.3228
  12. Lai EY, Chyau CC, Mau JL, et al (2004). Antimicrobial activity and cytotoxicity of the essential oil of Curcuma zedoaria. Am J Chin Med, 32, 281-90. https://doi.org/10.1142/S0192415X0400193X
  13. Li Y, Wo J, Liu Q, et al (2009). Chemoprotective effects of Curcuma aromatica on esophageal carcinogenesis. Ann Surg Oncol, 16, 515-23. https://doi.org/10.1245/s10434-008-0228-0
  14. Makabe H, Maru N, Kuwabara A, et al (2006). Antiinflammatory sesquiterpenes from Curcuma zedoaria. Nat Prod Res, 20, 680-5. https://doi.org/10.1080/14786410500462900
  15. Oh OJ, Min HY, Lee SK (2007). Inhibition of inducible prostaglandin E2 production and cyclooxy-genase-2 expression by curdione from Curcuma zedoaria, Arch Pharmacal Res, 30, 1226-39.
  16. Reed JC (1998). Bcl-2 family proteins. Oncogene, 17, 3225-36.
  17. Shiobara Y, Asakawa Y, Kodama M, et al (1985). Curcumenone, curcumanolide A and curcumanolide B, three sesquiterpenoids from Curcuma zedoaria. Phytochemistry, 24, 2629-33. https://doi.org/10.1016/S0031-9422(00)80683-4
  18. Shu B, Duan W, Yao J, et al (2009). Caspase 3 is involved in the apoptosis induced by triptolide in HK-2 cells. Toxicol In Vitro, 23, 598-602. https://doi.org/10.1016/j.tiv.2009.01.021
  19. Thornberry NA, Lazebnik Y (1998). Caspases: enemi es within. Science, 28, 1312-6.
  20. Xu LY, Tian LQ, Li K, et al (2007). Study on the volatile oil by SFE-CO, from crude and processed rhizoma atractylodis by GC-MS. Zhong Yao Cai, 30, 16-20.
  21. Yan J, Chen G, Tong S, et al (2005). Preparative isolation and purification of germacrone and curdione from the essential oil of the rhizomes of Curcuma wenyujin by high-speed counter-current chromatography. J Chromatogr A, 1070, 207-10. https://doi.org/10.1016/j.chroma.2005.02.064
  22. Zhong ZF, Chen XP, Tan W, et al (2011). Germacrone inhibits the proliferation of breast cancer cell lines by inducing cell cycle arrest and promoting apoptosis. Eur J Pharmacol, 667, 50-5. https://doi.org/10.1016/j.ejphar.2011.03.041
  23. Zhou J, Luo YH, Wang JR, et al (2013). Gambogenic acid induction of apoptosis in a breast cancer cell line. Asian Pac J Cancer Prev, 14, 7601-5. https://doi.org/10.7314/APJCP.2013.14.12.7601
  24. Zwaving JH, Bos R (1991). Analysis of the essential oils of five Curcuma species. Flavour Fragrance J, 7, 19-22.

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