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The Korean Society of Phycology (한국조류학회(藻類))
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Fucoxanthin derivatives from Sargassum siliquastrum inhibit matrix metalloproteinases by suppressing NF-κB and MAPKs in human fibrosarcoma cells

  • Nguyen, Van-Tinh (Department of Biomedical Engineering, and Centre for Marine-Integrated Biomedical Technology (BK21 Plus) Pukyong National University) ;
  • Qian, Zhong-Ji (College of Food Science and Technology, Guangdong Ocean University) ;
  • Lee, Bonggi (College of Pharmacy, Pusan National University) ;
  • Heo, Soo-Jin (Global Bioresources Research Center, Korea Institute of Ocean Science and Technology) ;
  • Kim, Kil-Nam (Marine Bio Research Team, Korea Basic Science Institute (KBSI)) ;
  • Jeon, You-Jin (Department of Marine Life Sciences, Jeju National University) ;
  • Park, Won Sun (Department of Physiology, Kangwon National University School of Medicine) ;
  • Choi, Il-Whan (Department of Microbiology, Inje University College of Medicine) ;
  • Jang, Chul Ho (Department of Otolaryngology, Chonnam National University Medical School) ;
  • Ko, Seok-Chun (Institute of Marine Biotechnology, Pukyong National University) ;
  • Park, Sun-Joo (Department of Chemistry, Pukyong National University) ;
  • Kim, Yong-Tae (Department of Food Science and Biotechnology, Kunsan National University) ;
  • Kim, GeunHyung (Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University) ;
  • Lee, Dae-Sung (Marine Biodiversity Institute of Korea) ;
  • Yim, Mi-Jin (Marine Biodiversity Institute of Korea) ;
  • Je, Jae-Young (Department of Marine-bio Convergence Science, Pukyong National University) ;
  • Jung, Won-Kyo (Department of Biomedical Engineering, and Centre for Marine-Integrated Biomedical Technology (BK21 Plus) Pukyong National University)
  • Received : 2014.05.20
  • Accepted : 2014.11.14
  • Published : 2014.12.15
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Abstract

Fucoxanthin is known to be an effective cell proliferation inhibitor with anti-tumor and anti-angiogenic activities. However, there is a lack of data regarding the biological effects of cis isomers of fucoxanthin. To assess the potential therapeutic properties of 9'-cis-(6'R) fucoxanthin (FcA), and 13-cis and 13'-cis-(6'R) fucoxanthin complex (FcB) isolated from Sarggassum siliquastrum, we investigated their inhibitory effects on matrix metalloproteinases (MMPs) in phorbol 12-myristate 13-acetate (PMA)-induced human fibrosarcoma (HT1080) cells. FcA and FcB reduced MMP-2 and MMP-9 protein and mRNA levels, as well as the migration of these cells, in a dose-dependent manner. Additionally, FcA and FcB increased levels of MMPs inhibition factors such as tissue inhibitor of metalloproteinase-1. FcA and FcB significantly inhibited the transcriptional activity of nuclear factor ${\kappa}B$ (NF-${\kappa}B$) and by inhibiting c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinases. Our results demonstrate that suppression of the NF-${\kappa}B$, JNK, and p38 signaling pathways may inhibit PMA-induced MMP-2 and MMP-9 activity. Therefore, FcA and FcB may be useful in noninvasive therapeutic strategies against fibrosarcoma metastasis.

Keywords

References

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