ALGAE

The Korean Society of Phycology (한국조류학회(藻類))
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5β-Hydroxypalisadin B isolated from red alga Laurencia snackeyi attenuates inflammatory response in lipopolysaccharide-stimulated RAW 264.7 macrophages

  • Wijesinghe, W.A.J.P. (Department of Export Agriculture, Faculty of Animal Science and Export Agriculture, Uva Wellassa University) ;
  • Kang, Min-Cheol (School of Marine Biomedical Sciences, Jeju National University) ;
  • Lee, Won-Woo (School of Marine Biomedical Sciences, Jeju National University) ;
  • Lee, Hyi-Seung (Marine Natural Products Laboratory, Korea Ocean Research & Development Institute) ;
  • Kamada, Takashi (Laboratory of Natural Products Chemistry, Institute for Tropical Biology and Conservation, University Malaysia Sabah) ;
  • Vairappan, Charles S. (Laboratory of Natural Products Chemistry, Institute for Tropical Biology and Conservation, University Malaysia Sabah) ;
  • Jeon, You-Jin (School of Marine Biomedical Sciences, Jeju National University)
  • Received : 2014.03.06
  • Accepted : 2014.09.16
  • Published : 2014.12.15
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Abstract

In this study, four compounds isolated from the red alga Laurencia snackeyi were evaluated for their potential anti-inflammatory effect in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. These compounds were tested for their inhibitory effects on nitric oxide (NO) production in LPS-stimulated RAW 264.7 cells. Since $5{\beta}$-hydroxypalisadin B showed the best activity it was further tested for the production of prostaglandin-$E_2$ ($PGE_2$), expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), the release of pro-inflammatory cytokines tumor necrotic factor-alpha (TNF-${\alpha}$), interleukin-$1{\beta}$ (IL-$1{\beta}$), and interleukin-6 (IL-6). $5{\beta}$-Hydroxypalisadin B significantly reduced the $PGE_2$ release and suppressed the iNOS and COX-2 expression in LPS-stimulated RAW 264.7 cells. It also significantly reduced the release of pro-inflammatory cytokines TNF-${\alpha}$, IL-$1{\beta}$, and IL-6. These findings provide the first evidence of anti-inflammatory potential of $5{\beta}$-hydroxypalisadin B isolated from the red alga L. snackeyi and hence, it could be exploited as an active ingredient in pharmaceutical, nutraceutical and functional food applications.

Keywords

References

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