Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Inhibitory Effects of Water Extract from Rice Bran Due to cAMP-dependent Phosphorylation of VASP ($Ser^{157}$) on ADP-induced Platelet Aggregation

  • Kim, Hyun-Hong (Department of Biomedical Laboratory Science, Inje University) ;
  • Hong, Jeong Hwa (Department of Smart Foods and Drugs, Inje University) ;
  • Ingkasupart, Pajaree (Department of Smart Foods and Drugs, Inje University) ;
  • Lee, Dong-Ha (Department of Biomedical Laboratory Science, Inje University) ;
  • Park, Hwa-Jin (Department of Biomedical Laboratory Science, Inje University)
  • Received : 2014.06.02
  • Accepted : 2014.09.27
  • Published : 2014.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, we investigated the effect of water extract from rice bran (RB) on ADP ($20{\mu}M$)-stimulated platelet aggregation. RB dose-dependently inhibited ADP-induced platelet aggregation, and its $IC_{50}$ value was $224.0{\mu}g/mL$, which was increased by adenylate cyclase inhibitor SQ22536 and cAMP-dependent protein kinase (A-kinase) inhibitor Rp-8-Br-cAMPS. RB elevated the phosphorylation of VASP ($Ser^{157}$) which was also inhibited by SQ22536 and Rp-8-Br-cAMPS. It is thought that RB-elevated cAMP contributed to the phosphorylation of VASP ($Ser^{157}$) to inhibit ADP-induced platelet aggregation. Therefore, we demonstrate that RB has an antiplatelet effect via cAMP-dependent phosphorylation of VASP ($Ser^{157}$), and RB may have preventive or therapeutic potential for platelet aggregation-mediated diseases, such as thrombosis, myocardial infarction, atherosclerosis, and ischemic cerebrovascular disease.

Keywords

References

  1. Barragan P, Bouvier JL, Roquebert PO, Macaluso G, Commeau P, Comet B, Lafont A, Camoin L, Walter U, Eigenthaler M. Resistance to thienopyridines: clinical detection of coronary stent thrombosis by monitoring of vasodilator-stimulated phosphoprotein phosphorylation. Catheter Cardiovasc Interv. 2003. 59: 295-302. https://doi.org/10.1002/ccd.10497
  2. Berridge MJ, Irvine RF. Inositol trisphosphate, a novel second messenger in cellular signal transduction. Nature. 1984. 312: 315-321. https://doi.org/10.1038/312315a0
  3. Butt E, Abel K, Krieger M, Palm D, Hoppe V, Hoppe J, Walter U. cAMP- and cGMP-dependent protein kinase phosphorylation sites of the focal adhesion vasodilator stimulated phosphoprotein (VASP) in vitro and in intact human platelets. J Biol Chem. 1994. 269: 14509-14517.
  4. Cattaneo M. The P2 receptors and congenital platelet function defects. Semin Thromb Hemost. 2005. 31: 168-173. https://doi.org/10.1055/s-2005-869522
  5. Gasser JA, Betterridge DJ. Comparison of the effects of carvedilol, propranolol, and verapamil on in vitro platelet function in healthy volunteers. J Cardiovasc Pharmacol. 1991. 8: S29-S34.
  6. Goufo P, Trindade H. Rice antioxidants: phenolic acids, flavonoids, anthocyanins, proanthocyanidins, tocopherols, tocotrienols, $\gamma$-oryzanol, and phytic acid. Food Sci Nutr. 2014. 2: 75-104. https://doi.org/10.1002/fsn3.86
  7. Guidetti GF, Lova P, Bernardi B, Campus F, Baldanzi G, Graziani A, Balduini C, Torti M. The Gi-coupled P2Y12 receptor regulates diacylglycerol-mediated signaling in human platelets. J Biol Chem. 2008. 283: 18352-18363.
  8. Halbrugge M, Walter U. Purification of a vasodilator-regulated phosphoprotein from human platelets. Eur J Biochem. 1989. 185: 41-50. https://doi.org/10.1111/j.1432-1033.1989.tb15079.x
  9. Halbrugge M, Friedrich C, Eigenthaler M, Schanzenbacher P, Walter U. Stoichiometric and reversible phosphorylation of a 46-kDa protein in human platelets in response to cGMP- and cAMP-elevating vasodilators. J Biol Chem. 1990. 265: 3088-3093.
  10. Hou Z, Qin R, Ren G. Effect of anthocyanin-rich extract from black rice (Oryza sativa L. Japonica) on chronically alcoholinduced liver damage in rats. J Agric Food Chem. 2010. 58: 3191-3196. https://doi.org/10.1021/jf904407x
  11. Jennings LK. Role of platelets in atherothrombosis. Am J Cardiol. 2009. 103: 4A-10A. https://doi.org/10.1016/S0002-9149(09)00558-X
  12. Jun HI, Song GS, Yang EI, Youn Y, Kim YS. Antioxidant activities and phenolic compounds of pigmented rice bran extracts. J Food Sci. 2012. 77: C759-C764. https://doi.org/10.1111/j.1750-3841.2012.02763.x
  13. Laurent V, Loisel TP, Harbeck B, Wehman A, Grobe L, Jockusch BM, Wehland J, Gertler FB, Carlier MF. Role of proteins of the Ena/VASP family in actin-based motility of Listeria monocytogenes. J Cell Biol. 1999. 144: 1245-1258. https://doi.org/10.1083/jcb.144.6.1245
  14. Lee DH, Cho HJ, Kim HH, Rhee MH, Ryu JH, Park HJ. Inhibitory effects of total saponin from Korean red ginseng via vasodilator-stimulated phosphoprotein-Ser(157) phosphorylation on thrombin-induced platelet aggregation. J Ginseng Res. 2013. 37: 176-186. https://doi.org/10.5142/jgr.2013.37.176
  15. Lee DH, Kim HH, Cho HJ, Bae JS, Yu YB, Park HJ. Antiplatelet effects of caffeic acid due to $Ca^{2+}$ mobilization-inhibition via cAMP-dependent inositol-1, 4, 5-trisphosphate receptor phosphorylation. J Atheroscler Thromb. 2014. 21: 23-37.
  16. Menshikov Myu, Ivanova K, Schaefer M, Drummer C, Gerzer R. Influence of the cGMP analog 8-PCPT-cGMP on agonistinduced increases in cytosolic ionized $Ca^{2+}$ and on aggregation of human platelets. Eur J Pharmacol. 1993. 245: 281-284. https://doi.org/10.1016/0922-4106(93)90108-L
  17. Ok WJ, Cho HJ, Kim HH, Lee DH, Kang HY, Kwon HW, Rhee MH, Kim M, Park HJ. Epigallocatechin-3-gallate has an anti-platelet effect in a cyclic AMP-dependent manner. J Atheroscler Thromb. 2012. 19: 337-348. https://doi.org/10.5551/jat.10363
  18. Qureshi AA, Sami SA, Khan FA. Effects of stabilized rice bran, its soluble and fiber fractions on blood glucose levels and serum lipid parameters in humans with diabetes mellitus Types I and II. J Nutr Biochem. 2002. 13: 175-187. https://doi.org/10.1016/S0955-2863(01)00211-X
  19. Schwartz SM, Heinmark RL, Majesky MW. Developmental mechanisms underlying pathology of arteries. Physiol Rev. 1990. 70: 1177-1209. https://doi.org/10.1152/physrev.1990.70.4.1177
  20. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdicphosphotungstic acid reagents. Am J Enol Vitic. 1965. 16: 144-158.
  21. Smolenski A, Bachmann C, Reinhard K, Honig-Liedl P, Jarchau T, Hoschuetzky H, Walter U. Analysis and regulation of vasodilator-stimulated phosphoprotein serine 239 phosphorylation in vitro and in intact cells using a phosphospecific monoclonal antibody. J Biol Chem. 1998. 273: 20029-20035. https://doi.org/10.1074/jbc.273.32.20029
  22. Sudo T, Ito H, Kimura Y. Phosphorylation of the vasodilatorstimulated phosphoprotein (VASP) by the anti-platelet drug, cilostazol, in platelets. Platelets. 2003. 14: 381-390. https://doi.org/10.1080/09537100310001598819
  23. Wang BH, Ou-Yang JP. Pharmacological actions of sodium ferulate in cardiovascular system. Cardiovasc Drug Rev. 2005. 23: 161-172.
  24. Yasuda T, Takasawa A, Nakazawa T, Ueda J, Ohsawa K. Inhibitory effects of urinary metabolites on platelet aggregation after orally administering Shimotsu-To, a traditional Chinese medicine, to rats. J Pharm Pharmacol. 2003. 55: 239-244. https://doi.org/10.1211/002235702531