미생물학회지 (Korean Journal of Microbiology)

  • 제50권4호
  • /
  • Pages.384-386
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  • 2014
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  • 0440-2413(pISSN)
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  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
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국제표준화기구 ISO/NP 16266 방법을 이용한 환경 중 Pseudomonas aeruginosa의 분리 및 동정

Isolation and Identification of Pseudomonas aeruginosa in Natural Environments by International Organization for Standardization ISO/NP 16266

  • 이시원 (국립환경과학원 상하수도연구과) ;
  • 김지혜 (국립환경과학원 상하수도연구과) ;
  • 이보람 (국립환경과학원 상하수도연구과) ;
  • 주윤리 (국립환경과학원 상하수도연구과) ;
  • 최별 (국립환경과학원 상하수도연구과) ;
  • 박수정 (국립환경과학원 상하수도연구과) ;
  • 정현미 (국립환경과학원 상하수도연구과) ;
  • 정원화 (국립환경과학원 상하수도연구과)
  • Lee, Siwon (Water Supply and Sewerage Research Division, National Institute of Environmental Research) ;
  • Kim, Ji Hye (Water Supply and Sewerage Research Division, National Institute of Environmental Research) ;
  • Lee, Bo-Ram (Water Supply and Sewerage Research Division, National Institute of Environmental Research) ;
  • Joo, Youn-Lee (Water Supply and Sewerage Research Division, National Institute of Environmental Research) ;
  • Choe, Byeol (Water Supply and Sewerage Research Division, National Institute of Environmental Research) ;
  • Park, Su Jeong (Water Supply and Sewerage Research Division, National Institute of Environmental Research) ;
  • Chung, Hyen-Mi (Water Supply and Sewerage Research Division, National Institute of Environmental Research) ;
  • Jheong, Weon Hwa (Water Supply and Sewerage Research Division, National Institute of Environmental Research)
  • 투고 : 2014.10.22
  • 심사 : 2014.12.24
  • 발행 : 2014.12.31
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초록

녹농균은 병원, 물, 토양 및 대기 등의 많은 자연과 인공환경에서 서식하는 기회주의적 병원체로, 병원 내 감염 등의 심각한 문제점을 야기할 수 있다. 본 연구에서는 신뢰높은 표준화 방법인 International Organization for Standardization (ISO)을 적용하여, 물과 토양에서 220개의 녹농균의 추정 집락을 분리하였으며, 생리생화학적 특성으로 녹농균을 동정하였다. ISO/NP 16266 방법을 적용하였을 때, 총 220개의 추정 집락 중 방류수에서 drain swab으로 채취한 오직 1개의 집락만이 최종적으로 녹농균 양성으로 확정되었다. 한편, ISO에서 2014년 새롭게 제안한 1,10 phenanthroline 저항성 실험은, 환경 중 P. aeruginosa를 검출 및 동정하기 위해 적합한 방법으로 사료된다.

Pseudomonas aeruginosa is an opportunistic pathogen that inhabits various natural and artificial environments, such as pathogenesis, water, soil and air. They can cause serious problems, such as pathogenic infection. In this study, 220 colonies were isolated from water and soil environment that assumed to be P. aeruginosa using a membrane filter method based on International Organization for Standardization (ISO/NP 16266). Identification of the isolates was determined by physiobiochemical characteristics using newly modified ISO method which includes the resistance to 1,10 phenanthroline test. Only one of 220 presumed P. aeruginosa strains isolated from effluence water using a drain swab was determined as P. aeruginosa-positive by the ISO/NP 16266 method. Subsequently, the resistance to 1,10 phenanthroline test, which was newly proposed by ISO in 2014 and applied in this study, was considered as more precise and improvable method for identification of P. aeruginosa.

키워드

참고문헌

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피인용 문헌

  1. Analysis of Bacterial Diversity in Water from the Han River Water Source Protection Area via a Pyrosequencing Assay vol.42, pp.4, 2016, https://doi.org/10.5668/JEHS.2016.42.4.274