Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Analysis of Epidemiological Characteristics, PFGE Typing and Antibiotic Resistance of Pathogenic Escherichia coli Strains Isolated from Gyeonggi-do

경기도에서 분리한 병원성대장균의 역학적 특성 및 PFGE, 항생제 내성 연구

  • Kim, Kyung-A (Team of Microbiology, Gyeonggi Institute of Health and Environment) ;
  • Yong, Kum-Chan (Team of Microbiology, Gyeonggi Institute of Health and Environment) ;
  • Jeong, Jin-A (Team of Microbiology, Gyeonggi Institute of Health and Environment) ;
  • Huh, Jeong-Weon (Team of Microbiology, Gyeonggi Institute of Health and Environment) ;
  • Hur, Eun-Seon (Team of Microbiology, Gyeonggi Institute of Health and Environment) ;
  • Park, Sung-Hee (Team of Microbiology, Gyeonggi Institute of Health and Environment) ;
  • Choi, Yun-Sook (Team of Microbiology, Gyeonggi Institute of Health and Environment) ;
  • Yoon, Mi-Hye (Team of Microbiology, Gyeonggi Institute of Health and Environment) ;
  • Lee, Jong-Bok (Team of Microbiology, Gyeonggi Institute of Health and Environment)
  • 김경아 (경기도보건환경연구원 미생물팀) ;
  • 용금찬 (경기도보건환경연구원 미생물팀) ;
  • 정진아 (경기도보건환경연구원 미생물팀) ;
  • 허정원 (경기도보건환경연구원 미생물팀) ;
  • 허은선 (경기도보건환경연구원 미생물팀) ;
  • 박성희 (경기도보건환경연구원 미생물팀) ;
  • 최연숙 (경기도보건환경연구원 미생물팀) ;
  • 윤미혜 (경기도보건환경연구원 미생물팀) ;
  • 이정복 (경기도보건환경연구원 미생물팀)
  • Received : 2014.08.29
  • Accepted : 2014.11.26
  • Published : 2014.12.31
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Abstract

This study was conducted to survey the epidemiological characteristics and the isolated strains for pathogenic E. coli which was the major causative organisms for food poisoning occurred at school food services in the Gyeonggi-do area during the past three years. We investigated 19 accidents of food-borne disease outbreaks by pathogenic E. coli at school food services from 2010 to 2012. Food-borne disease outbreaks by pathogenic E. coli were usually occurred at direct management type (18 accidents, 95%) and high schools. For the seasonal factors, 13 accidents (65%) were occurred in June to September, especially the end of August and September after the summer holidays. The first patients were occurred on Wednesday (7 accidents, 37%) and Thursday (7 accidents, 37%), and they were mainly reported on Thursday (7 accidents, 37%) and Friday (5 accidents, 26%). The exposure of risk was estimated in Monday (4 accidents, 21%), Tuesday (7 accidents, 37%) and Wednesday (4 accidents, 21%), and kimchi (5 accidents, 50%) was estimated as the food of the high risk responsible for the outbreaks. 98 isolates of pathogenic E. coli consisted of PEC (50%), ETEC (34%), EAEC (15%), and EHEC (1%). The antibiotic resistance of pathogenic E. coli showed in the descending order of ampicilline (40%), nalidixic acid (37%), trimethoprim/sulfamethoxazole (24%), and tetracycline (19%). The antibiotics of second and third generation cephalosporins, cabarpenem, aminoglycosides, and second generation quinolones had antimicrobial susceptibilities and cefalotin, ampicillin/sulbactam and chloramphenicol showed medium resistance at 29%, 25%, and 6% respectively, and 70% of isolates were resistant to more than one antibiotic. By the PFGE analysis, they were classified into nine major groups and 31 profiles with 57% pattern similarity. It was very difficult to find the correlation of antimicrobial susceptibilities and genotype in the small scale-food poisoning, but the similarity of antimicrobial resistance and PFGE patterns in the large scale-food poisoning enabled the outbreaks to estimate the same pathotype of E. coli derived from identical origins.

병원성 대장균은 최근 3년간 경기도에서 발생한 학교급식식 중독의 주된 원인균으로서 그 역학적 특성을 정리하고 PFGE typing과 항생제 감수성 시험으로 분리균주의 특성을 알아보고자 하였다. 2010년부터 2012년까지 19건의 학교급식 식중독에 대한 역학조사보고서를 분석한 결과, 급식의 주된 운영방식은 직영(18건, 95%)이며, 고등학교에서 주로 발생하였다. 계절적 요인으로는 13건(65%)이 6월부터 9월에, 특히 여름방학 후 학기 초인 8월말부터 9월에 더 많이 발생하였다. 수요일(7건, 37%)과 목요일(7건, 37%)에 최초환자가 발생되어, 목요일 7건(37%) 금요일 5건(26%)에 주로 신고가 이루어 졌다. 추정위험에는 월요일(4건, 21%), 화요일(7건, 37%), 수요일(4건, 21%)에 노출되었고, 추정원인체로서 가능성이 높은 식품은 김치류가 50%(5건)를 차지하였다. 분리된 98균주는 EPEC, ETEC, EAEC, EHEC가 각각 50%, 34%, 15%, 1%이며, 항생제 감수성 시험 결과 ampicillin, nalidixic acid, sulfamethoxazole/trimethoprim, tetracycline에 대하여 각각 40%, 37%, 24%, 19%의 내성을 나타내었고, 2, 3세대 cephalosporins계, cabarpenem계, aminoglycosides계, 2세대 quinolones계 항생제에는 감수성이 있었으며, cefalotin, ampicillin/sulbactam, chloramphenicol에는 각각 29%, 25%, 6%가 중간내성을, 그리고 70%에서 한 가지 이상의 항생제에 내성이 관찰되었다. PFGE 분석결과, 57.6%의 similarity로 8개의 group과 32개의 profiles로 분류되었다. 개별적인 소규모 식중독에서 항생제 감수성과 유전자형의 연관성을 찾기가 어려웠으며, 동시에 발생한 대규모 식중독의 경우, 항생제 내성과 PFGE 유형이 매우 유사하여 동일 기원으로부터 유래한 병원성대장균에 의해 식중독 사고가 발생한 것으로 추정이 가능하였다.

Keywords

References

  1. Bentley, R. and Meganathan, R. 1982. Biosynthesis of vitamin K (menaquinone) in Bacteria. Bacteriol. Rev. 46, 241-280.
  2. Dixon B. 2000. Antimicrobials as growth promoters : Risks and alternatives. ASM News 66, 264-265.
  3. Dupont, H.L., Formal, S.B., Hornick, R.B., Snyder, M.J., Libonati, J.P., and Sheahan, D.G. 1971. Pathogenesis of Escherichia coli diarrhea. N. Engl. J. Med. 285, 1-9. https://doi.org/10.1056/NEJM197107012850101
  4. e-Country Indicators. 2012. http://www.index.go.kr/search/search.jsp.
  5. Finlay, B.B., Rosenshine, I., Donnenberg, M.S., and Kaper, J.B. 1992. Cytoskeletal composition of attaching and effacing lesions associated with enteropathogenic Escherichia coli adherence to HeLa cells. Infect. Immun. 60, 2541-2543.
  6. Hudault, S., Guignot, J., and Servin, A.L. 2001. Escherichia coli strains colonizing the gastrointestinal tract protect germfree mice against Salmonella typhimurium infection. Gut 49, 47-55. https://doi.org/10.1136/gut.49.1.47
  7. Huh, M.J., Oh, S.S., and Jang, J.S. 2013. Antimicrobial resistance and implicated genes of E. coli isolated from commercial and cooked foods in Seoul. Korean J. Food Nutr. 26, 132-136. https://doi.org/10.9799/ksfan.2013.26.1.132
  8. Jin, Y.H., Seung, H.J., Oh, Y.H., Jung, J.H., Jeon, S.J., Lee, J.K., Kim, C.K., Choi, S.M., and Chae, Y.Z. 2013. Epidemiological relationship of enterotoxigenic Escherichia coli and enteroaggregative E. coli isolated from patients with diarrhea in Seoul. J. Bacteriol. Virol. 43, 37-44. https://doi.org/10.4167/jbv.2013.43.1.37
  9. Jo, S.H., Kim, C.l., and Ha, S.D. 2009. Outbreak pattern forecasting of food-borne disease in group food services in Korea. J. Fd. Hyg. Safety 24, 19-26.
  10. Jung, S.C. 2005. Screening of antimicrobial resistance. The 3rd national antimicrobial resistance safety management and vision. Korea Food and Drug Administration, pp. 113-126.
  11. Kaper, J.B., Nataro, J.P., and Mobley, H.L.T. 2004. Pathogenic Escherichia coli. Nat. Rev. Microbiol. 2, 123-140. https://doi.org/10.1038/nrmicro818
  12. Kim, J.H., Oh, B.Y., Gong, Y.W., Kim, H.Y., Lee, M.Y., Koh, Y.J., Hwang, K.W., Jegal, S., and Lee, J.M. 2010. The trend of antimicrobial resistance of Escherichia coli isolated from healthy volunteers of community and hospital patients in Incheon. Korean J. Microbiol. 42, 252-256.
  13. Kim, Y.J., Chun, H.J., Lee, J.W., Hong, K.W., Kim, S.I., Wie, S.H., Kim, Y.R., and Kang, M.W. 2012. The changing patterns of antibiotics usage in Korea during 1981-2008. Infect. Chemother. 44, 411-418. https://doi.org/10.3947/ic.2012.44.6.411
  14. Korea Food Safety Research Institute. 2013. Media Workshop 2013 12th school meals and Safety. http://www.kfsri.or.kr/.
  15. Korea Ministry of Agriculture, Food and Rural Affairs. 2013. http://www.mafra.go.kr https://www.mafra.go.kr/list.jsp?&newsid= 155444846§ion_id=b_sec_1&pageNo=88&year=2014&list cnt=10&board_kind=C&board_skin_id=C3&depth=1&division =B&group_id=3&menu_id=1125&reference=2&parent_code=3 &popup_yn=N&tab_yn=N.
  16. Korea Ministry of Food and Drug Safety. 2012a. http://www.mfds.go.kr/ e-stat/index.do.
  17. Korea Ministry of Food and Drug Safety. 2012b. http://www.mfds.go.kr/ index.do?mid= 664&pageNo=92&seq=17557&cmd=v.
  18. Korea Ministry of Health and Welfare. 2009. http://www.mw.go.kr/ front_new/al/sal0301vw.jsp?PAR_MENU_ID=04&MENU_ID= 0403&BOARD_ID=140&BOARD_FLAG=00&CONT_SEQ=2 17331&page=1
  19. Knutton, S. 1994. Attaching and effacing E. coli. In Gyles, C.L. (ed.), Escherichia coli in domestic animals and humans, pp. 567-591. CAB International, Wallingford.
  20. Lee, K.M. and Ryu, K. 2007. Field assessment of sanitation management for school foodservice suppliers in the Seoul area. Kor. J. Food Cookery Sci. 23, 650-663.
  21. McDaniel, T.K., Jarvis, K.G., Donnenberg, M.S., and Kaper, J.B. 1995. A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens. Proc. Natl. Acad. Sci. USA 92, 1664-1668. https://doi.org/10.1073/pnas.92.5.1664
  22. Nataro, J.P. and Kaper, J.B. 1998. A comprehensive review of the pathogenesis, epidemiology, diagnosis and clinical aspects of diarrhoeagenic E. coli. Clin. Microbiol. Rev. 11, 142-201.
  23. Ochiai, K., Yamanaka, T., Kimura, K., and Sawada, O. 1959. Inheritance of drug resistance (and its transfer) between Shigella strains and between Shigella and E. coli strains. Hihon Iji Shimpor 1861, 34.
  24. People's solidarity for participatory democracy. Antibiotics Usage monitoring at livestock industry and aquaculture II. 2006. http://www.peoplepower21.org/StableLife/629493.
  25. Pupo, G.M., Lan, R., and Reeves, P.R. 2000. Multiple independent origins of Shigella clones of Escherichia coli and convergent evolution of many of their characteristics. Proc. Natl. Acad. Sci. USA 97, 10567-10572. https://doi.org/10.1073/pnas.180094797
  26. Reid, G., Howard, J., and Gan, B.S. 2001. Can bacterial interference prevent infection? Trends Microbiol. 9, 424-428. https://doi.org/10.1016/S0966-842X(01)02132-1
  27. Sansonetti, P. 2002. Host-pathogen interactions: the seduction of molecular cross talk. Gut 50, 2-8.
  28. Scaletsky, I.C.A., Fabbricotti, S.H., Carvalho, R.L.B., Nunes, C.R., Maranha, H.S., Morais, M.B., and Fagundes-Neto, U. 2002. Diffusely adherent Escherichia coli as a cause of acute diarrhea in young children in northeast Brazil: a case-control study. J. Clin. Microbiol. 40, 645-648. https://doi.org/10.1128/JCM.40.2.645-648.2002
  29. Sung, M.S., Kim, J.H., and Kim, K.S. 2010. Molecular epidemiologic analysis of pathogenic Escherichia coli isolated from poultry in Korea. Kor. J. Vet. Res. 50, 239-246.
  30. Wei, J., Goldberg, M.B., Burland V., Venkatesan, M.M., Deng, W., Fournier, G., Mayhew, G.F., Plunkett III, G., Rose, D.J., Darling, A., and et al. 2003. Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T. Infect. Immun. 71, 2775-2786. https://doi.org/10.1128/IAI.71.5.2775-2786.2003
  31. Wolfgang, W. 1998. Medical consequences of antibiotic use in agriculture. Science 279, 996-997. https://doi.org/10.1126/science.279.5353.996
  32. Yoo, Y.A., Kim, M.S., Kim, K.S., Park, S.H., and Jung, S.K. 2010. Antimicrobial resistance and implicated genes of E. coli isolated from commercial and cooked foods in Seoul. J. Fd. Hyg. Safety 25, 220-225.

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