Journal of Biomedical Engineering Research (대한의용생체공학회:의공학회지)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
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Fabrication and Characterization of 3-D Porous Collagen Scaffold

3차원 다공성 콜라겐지지체의 제조 및 특성 분석

  • Kim, Jin-Tae (Department of Advanced Material Engineering, Chungbuk National University) ;
  • Lim, Sumin (R&D Center, Neobiotech Co. Ltd.) ;
  • Kim, Byoung Soo (R&D Center, Neobiotech Co. Ltd.) ;
  • Lee, Deuk Yong (Deaprtment of Biomedical Engineering, Daelim University) ;
  • Choi, Jae Ha (Department of Advanced Material Engineering, Chungbuk National University)
  • 김진태 (충북대학교 신소재공학과) ;
  • 임수민 ((주)네오바이오텍 R&D센터) ;
  • 김병수 ((주)네오바이오텍 R&D센터) ;
  • 이득용 (대림대학교 의공융합과) ;
  • 최재하 (충북대학교 신소재공학과)
  • Received : 2014.09.05
  • Accepted : 2014.10.21
  • Published : 2014.12.30
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Abstract

Collagen scaffolds were synthesized by cross linking into a solution mixture of 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochlorid(EDC) in ethanol, followed by pressing, cleaning and lyophilization process after the type I atelo-collagen solutions in D.I water(pH3). The experimental conditions are collagen concentration of 1.0 wt%, 3.0 wt%, 5.0 wt% and differential concentration of cross-linker. Then, parametric studies were performed by varying the parameters to investigate the morphology, the porosity, the swelling ratio and the thickness and genotoxicity of the scaffolds. The scaffolds thickness pattern was regular to concentration of the degree of cross-linker and collagen. It was observed that the swelling ratio, the degree of crosslink, and the pore size(thickness of scaffold) can be controlled by adjusting the collagen, crosslinker. Among the parameters investigated, the smallest thickness can be achieved by collagen, crosslinker concentrate condition. The collagen scaffold is induced no genotoxicity. The lowest swelling ratio, as an indication of the highest degree of crosslink, can be obtained by adding crosslink agent.

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References

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