DOI QR코드

DOI QR Code

Effects of repeated tuberculin skin testings on immune responses in experimental monkeys

  • Min, Fangui (Guangdong Laboratory Animals Monitoring Institute) ;
  • Wang, Jing (Guangdong Laboratory Animals Monitoring Institute) ;
  • Yuan, Wen (Guangdong Laboratory Animals Monitoring Institute) ;
  • Kuang, Huiwen (Guangdong Laboratory Animals Monitoring Institute) ;
  • Zhao, Weibo (Guangdong Laboratory Animals Monitoring Institute)
  • Received : 2014.06.13
  • Accepted : 2014.11.19
  • Published : 2014.12.31

Abstract

Though many alternative methods to tuberculin skin testing (TST) have been established and evaluated in recent years, sensitivities and specificities of most methods could not meet the requirements of golden standards. In this study, we sought to identify whether repeated TSTs could affect the immune responses in experimental monkeys. Nine natural tuberculosis (TB) monkeys receiving repeated TSTs biweekly were used to demonstrate the effect on TST responsiveness. Two healthy monkeys were administrated with repeated TSTs to analyze the immune response profiling. Intrapalpebral reactions in TB infections gradually weakened or presented intermittent positive reactions. The leukocyte counts, cytokine responses, and antibody responses to all antigens except Old tuberculin (OT) and MPT64L showed no specific changes for TB in healthy monkeys. Positive antibody responses to OT and MPT64L emerged during the first half experimental period, which may cause by their cross-reactivity with mycobacterial species. Results showed that repeated TSTs had no significant effects on immune responses in healthy monkeys but a progressive reduction in TST responsiveness in TB infections.

Keywords

References

  1. Centers for Disease Control and Prevention: Tuberculosis in imported nonhuman primates: United States, June 1990 May 1993. MMWR Morb Mortal Wkly Rep 1993, 42:572-576.
  2. McMurray DN: A nonhuman primate model for preclinical testing of new tuberculosis vaccines. Clin Infect Dis 2000, 30(Suppl 3):S210-S212. https://doi.org/10.1086/313885
  3. Lerche NW, Yee JL, Capuano SV, Flynn JL: New approaches to tuberculosis surveillance in nonhuman primates. ILAR J 2008, 49(2):170-178. https://doi.org/10.1093/ilar.49.2.170
  4. Ryan TJ, Buddle BM, de Lisle GW: An evaluation of the gamma interferon test for detecting Bovine tuberculosis in cattle 8 to 28 days after tuberculin skin testing. Res Vet Sei 2000, 69(1):57-61. https://doi.org/10.1053/rvsc.2000.0386
  5. National Research Council: Guide for the Care and Use of Laboratory Animals. 8th edition. Washington, DC: National Academy Press; 2010.
  6. Capuano SV 3rd, Croix DA, Pawar S, Zinovik A, Myers A, Lin PL, Bissel S, Fuhrman C, Klein E, Flynn JL: Experimental Mycobacterium tuberculosis infection of cynomolgus macaques closely resembles the various manifestations of human. M Tuberculosis Infection Infect Immun 2003, 71(10):5831-5844.
  7. Min FG, Zhang Y, Huang R, Li WD, Wu Y, Pan JC, Zhao WB, Liu XM: Serum antibody responses to 10 Mycobacterium tuberculosis proteins, purified protein derivative, and old tuberculin in natural and experimental tuberculosis in rhesus monkeys. Clin Vaccine Immunol 2011, 18(12):2154-2160. https://doi.org/10.1128/CVI.05329-11
  8. Min FG, Zhang Y, Pan JC, Wang J, Yuan W: Mycobacterium tuberculosis infection in rhesus monkeys (Macaca mulatta) and evaluation of ESAT-6 and CFP10 as immunodiagnostic antigens. Exp Anim 2013, 62(4):281-293. https://doi.org/10.1538/expanim.62.281
  9. Smithburn KC, Sabin FR, Hummel LE: 1937. Haematological studies in experimental tuberculosis: variations in the blood cells of rabbits inoculated with cultures differing in virulence. American Review of Tuberculosis XXXVI. 673-691. Article. 19 Images.
  10. Harboe M: Antigen of PPD, old tuberculin and autoclaved mycobacterium bovis BCG studied by Immunoelectrophoresis. Am Rev Respir Dis 1981, 124:80-87.
  11. Weldingh K, Rosenkrands I, Okkels LM, Doherty TM, Andersen P: Assessing the serodiagnostic potential of 35 Mycobacterium tuberculosis proteins and identification of four novel serological antigens. J Clin Microbiol 2005, 43(1):57-65 https://doi.org/10.1128/JCM.43.1.57-65.2005