International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Antibody derived from insect glycosaminoglycan

  • Ahn, Mi Young (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Hwang, Jae Sam (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Yoon, Hyung Joo (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Yun, Eun Young (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
  • Received : 2014.12.10
  • Accepted : 2014.12.29
  • Published : 2014.12.31
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Abstract

We prepared antibodies from insect glycosaminoglycans (GAGs) and assayed the titer. Nine polyclonal antibodies against insect GAGs were raised for development of an ELISA in biological fluids (mice serum). The 3th booster collection of antiserum of BALB/c mice as a primarily antibody was assayed for titer determination by ELISA method. In sandwich ELISA of GAGs derived from Isaria sinclairii or other insects, antiserum from insect GAGs gave satisfactory results for so potent antibody(100: 1~1000:1) raising (manufacturing) agent in range of 10 ng/ml.

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References

  1. Ahn MY, Han JW, Yoon HJ, Hwang JS, Young YE (2012) Antiinflammatory effect of bumblebee alcohol extracts in CFA-induced rat. Toxicological Research 28(4), 249-253 https://doi.org/10.5487/TR.2012.28.4.249
  2. Ahn MY, Jung YS, Jee SD, Han JW, Hwang JS, Yun EY, Lee BM (2013) Blood pressure lowering effect of the glycosaminoglycan derived from Isaria sinclairii in SHR rats. J Tox Environ Health, Part A 76(4), 391-399. https://doi.org/10.1080/15287394.2013.765370
  3. Choi YJ, Hahn BS, Ahn MY, Park PK, Sohn JH, Choi ES, Lee SK, Kim YS (1997) Quantitation of recombinant hirudin by enzyme-linked immunosorbant assay. Yakhaki Hoeji 41(1), 74-80.
  4. Kim HH, Lee KP, Kato K, Arata Y (1996) Paratope mapping of Anti-Ex-A IgG as studied by NMR. Yakhaki Hoeji 40(4), 422-417.